{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T10:51:33Z","timestamp":1793875893935,"version":"4.7.0"},"reference-count":47,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T00:00:00Z","timestamp":1753660800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Project of Pudong New Area Health Commission","award":["PDZY-2025-0725 and PW2022D-14"],"award-info":[{"award-number":["PDZY-2025-0725 and PW2022D-14"]}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["21Y11923200"],"award-info":[{"award-number":["21Y11923200"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["23Y11921800"],"award-info":[{"award-number":["23Y11921800"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012472","name":"Education and Scientific Research Project of Shanghai","doi-asserted-by":"publisher","award":["202340278"],"award-info":[{"award-number":["202340278"]}],"id":[{"id":"10.13039\/501100012472","id-type":"DOI","asserted-by":"publisher"}]},{"name":"14th Five-Year Plan for the development of specialties in Traditional Chinese Medicine and the enhancement of emergency capabilities in TCM","award":["ZYTSZK1-8"],"award-info":[{"award-number":["ZYTSZK1-8"]}]},{"name":"Pudong New District Famous Traditional Chinese Medicine Heir Training Project","award":["PDZY-2025-0942"],"award-info":[{"award-number":["PDZY-2025-0942"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:sec id=\"sec032\">\n<jats:title>Introduction<\/jats:title>\n<jats:p>Delayed wound healing (DWH) following anal fistula surgery is a common complication that prolongs recovery, increases patient morbidity, and imposes significant healthcare costs. Potential risk factors such as diabetes, smoking, fistula complexity, and surgical techniques have been suggested in individual studies, yet no comprehensive synthesis exists to guide clinical practice. This study aims to identify and evaluate risk factors associated with DWH after anal fistula surgery by combining existing evidence and grading the evidence.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec033\">\n<jats:title>Methods and analysis<\/jats:title>\n<jats:p>This study will follow the Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols (PRISMA-P) guidelines. We will search PubMed, Embase, Cochrane Library, Web of Science, and grey literature databases from inception to March 2025, with no language restrictions. Observational studies (cohort and case-control) reporting risk factors for DWH, defined as incomplete healing beyond 6\u201312 weeks post-surgery, will be included. Two independent reviewers will screen titles\/abstracts, perform full-text reviews, extract data regarding study design, sample size, risk factors and outcomes, and assess risk of bias using the Newcastle-Ottawa Scale (NOS). Primary outcomes will include odds ratios (OR) or relative risks (RR) for factors such as comorbidities, lifestyle factors, and operative approaches. A random-effects meta-analysis will pool effect estimates if heterogeneity (I\u00b2\u2009&lt;\u200950%) permits; otherwise, a narrative synthesis will be conducted. Subgroup analyses will explore differences by study design and patient characteristics, with publication bias assessed using funnel plots and Egger\u2019s test. The certainty of evidence will be evaluated with the Grading of Recommendations Assessment, Development and Evaluation (GRADE) approach.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec034\">\n<jats:title>Discussion<\/jats:title>\n<jats:p>This study\u2019s strengths include its comprehensive search strategy and rigorous methodology, providing a robust synthesis of risk factors. Clinically, identifying modifiable risk factors could enhance preoperative optimization and postoperative care, reducing delayed healing rates. Future studies should standardize definitions of delayed healing and explore under-investigated factors like wound care techniques or microbiome influences to refine risk prediction models.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec035\">\n<jats:title>Registration<\/jats:title>\n<jats:p>PROSPERO CRD420251013602<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0329030","type":"journal-article","created":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T17:32:28Z","timestamp":1753723948000},"page":"e0329030","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":6,"title":["Risk factors for delayed wound healing after anal fistula surgery: Protocol of a meta-analytic study"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5012-957X","authenticated-orcid":true,"given":"Zubing","family":"Mei","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peixin","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yin","family":"Qu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huili","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiyan","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingming","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"De","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,7,28]]},"reference":[{"issue":"8","key":"pone.0329030.ref001","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1097\/DCR.0000000000002473","article-title":"The American Society of Colon and Rectal Surgeons Clinical Practice Guidelines for the Management of Anorectal Abscess, Fistula-in-Ano, and Rectovaginal Fistula","volume":"65","author":"WB Gaertner","year":"2022","journal-title":"Dis Colon Rectum"},{"issue":"4","key":"pone.0329030.ref002","first-page":"219","article-title":"Fistula-in-ano in a defined population. 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