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Therefore, the objective of this study was to evaluate the association of different navel care (NC) practices on the occurrence of omphalitis in neonatal dairy calves. Between December 2016 and July 2019, 196 dairy farms in Eastern Germany were visited once within a large-scale cross-sectional study. 1,967 calves aged five to 21 days were clinically examined, including palpation of the external umbilicus for inflammation signs. Furthermore, information on animal health and farm management, including the implementation of NC, was obtained through interviews with the farm or herd manager. Causal diagrams were drawn, containing variables considering NC (practice of NC, method of application, preparation applied, frequency of NC, time of first NC, wearing gloves during NC) as influence variables, omphalitis as target variable, and all potential confounders to perform multivariable statistical analyses at animal level. Over one-fourth of all calves examined showed omphalitis signs (n\u2009=\u2009525 calves, 26.7%). The odds of omphalitis tended to increase (OR = 2.3) if no NC was performed compared to regular NC. Almost half of all other variables analysed seemed relevant for the occurrence of omphalitis. Administering the preparation into the umbilical cord reduced the odds of omphalitis by 62% compared to no NC. Repeated applications tended to decrease the odds of omphalitis by 44% compared to single applications. Furthermore, wearing gloves during NC tended to increase the odds of omphalitis by 30% compared to not wearing gloves. Neither the preparation applied, the method of application, nor the timing of NC had an impact on the omphalitis occurrence. Considering these results, different NC practices influence the odds of omphalitis in neonatal dairy calves. 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mortality-Causes, risk factors, incidences, sequelae and prevention","author":"JF Mee","year":"2023","journal-title":"Reprod Domest Anim"},{"key":"pone.0329326.ref012","doi-asserted-by":"crossref","first-page":"1480851","DOI":"10.3389\/fvets.2024.1480851","article-title":"Risk factors for omphalitis in neonatal dairy calves","volume":"11","author":"KK Meier","year":"2024","journal-title":"Front Vet Sci"},{"issue":"4","key":"pone.0329326.ref013","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1080\/00480169.1993.35763","article-title":"A pathological and microbiological evaluation of omphalophlebitis in very young calves slaughtered in New Zealand","volume":"41","author":"SC Hathaway","year":"1993","journal-title":"N Z Vet J"},{"key":"pone.0329326.ref014","unstructured":"Lemaire C, Otz P, Arcangioli MA. Risk factors of calf omphalitis: a clinical study. In: European Buiatrics Forum, Roma, 2021."},{"key":"pone.0329326.ref015","volume-title":"Investigating physiological parameters and management procedures in the periparturient period that affect subsequent performance in ungulate species","author":"AL Fordyce","year":"2018"},{"issue":"8","key":"pone.0329326.ref016","doi-asserted-by":"crossref","first-page":"5726","DOI":"10.3168\/jds.2014-9235","article-title":"Short communication: The effect of 4 antiseptic compounds on umbilical cord healing and infection rates in the first 24 hours in dairy calves from a commercial herd","volume":"98","author":"AL Robinson","year":"2015","journal-title":"J Dairy Sci"},{"issue":"1","key":"pone.0329326.ref017","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.cvfa.2007.10.002","article-title":"Newborn dairy calf management","volume":"24","author":"JF Mee","year":"2008","journal-title":"Vet Clin North Am Food Anim Pract"},{"issue":"6","key":"pone.0329326.ref018","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1590\/1678-4162-12344","article-title":"Risk factors related to the appearance of umbilical disorders in dairy calves","volume":"73","author":"JA Bombardelli","year":"2021","journal-title":"Arq Bras Med Vet Zootec"},{"issue":"02","key":"pone.0329326.ref019","doi-asserted-by":"crossref","first-page":"185","DOI":"10.4236\/ojas.2015.52021","article-title":"A study of dairy heifer rearing practices from birth to weaning and their associated costs on UK dairy farms","volume":"05","author":"AC Boulton","year":"2015","journal-title":"OJAS"},{"key":"pone.0329326.ref020","unstructured":"Gamage B. A guide to selection and use of disinfectants. Available from: http:\/\/www.mtpinnacle.com\/pdfs\/disinfectant-selectionguidelines.pdf. 2003."},{"issue":"1","key":"pone.0329326.ref021","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1128\/CMR.12.1.147","article-title":"Antiseptics and disinfectants: activity, action, and resistance","volume":"12","author":"G McDonnell","year":"1999","journal-title":"Clin Microbiol Rev"},{"issue":"10","key":"pone.0329326.ref022","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1016\/j.ajic.2010.05.019","article-title":"Povidone-iodine against sodium hypochlorite as skin antiseptics in volunteers","volume":"38","author":"JA Alvarez","year":"2010","journal-title":"Am J Infect Control"},{"key":"pone.0329326.ref023","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1093\/biolreprod\/52.monograph_series1.77","article-title":"Effects of disinfectant treatments on the bacterial flora of the umbilicus of neonatal foals1","volume":"52","author":"RP Lavan","year":"1995","journal-title":"Biology of Reproduction"},{"issue":"1","key":"pone.0329326.ref024","first-page":"25","article-title":"Iodine antiseptics are not harmless","volume":"53","author":"J Arena Ansotegui","year":"2000","journal-title":"An Esp Pediatr"},{"key":"pone.0329326.ref025","unstructured":"Anonymous. Vet-Sept L\u00f6sung 10%. Available from: https:\/\/medicines.health.europa.eu\/veterinary\/en\/600000072904."},{"key":"pone.0329326.ref026","unstructured":"Anonymous. Vet-Sept Spray. Available from: https:\/\/medicines.health.europa.eu\/veterinary\/en\/600000073271."},{"key":"pone.0329326.ref027","unstructured":"Serumwerk Bernburg AG. Alkoholische Jodl\u00f6sung WDT. Available from: https:\/\/www.serumwerk.com\/wp-content\/uploads\/2018\/03\/AlkoholischeJodloesung-PI-02-18.pdf."},{"issue":"126","key":"pone.0329326.ref028","first-page":"35920","article-title":"Changes in the regulation of iodine crystals and chemical mixtures containing over 2.2 percent iodine. Final rule","volume":"72","author":"Drug Enforcement Administration (DEA), Justice","year":"2007","journal-title":"Fed Regist"},{"issue":"7","key":"pone.0329326.ref029","first-page":"414","article-title":"Umbilical disinfection options in large animal neonates","volume":"11","author":"K Dunne","year":"2021","journal-title":"Vet Ireland J"},{"issue":"3","key":"pone.0329326.ref030","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1111\/j.1742-481X.2007.00405.x","article-title":"Povidone-iodine: use in hand disinfection, skin preparation and antiseptic irrigation","volume":"5","author":"P Durani","year":"2008","journal-title":"Int Wound J"},{"issue":"5","key":"pone.0329326.ref031","article-title":"Umbilical cord antiseptics for preventing sepsis and death among newborns","volume":"2013","author":"A Imdad","year":"2013","journal-title":"Cochrane Database Syst Rev"},{"issue":"2","key":"pone.0329326.ref032","doi-asserted-by":"crossref","first-page":"152","DOI":"10.3855\/jidc.7224","article-title":"Impact of different antiseptics on umbilical cord colonization and cord separation time","volume":"11","author":"H Ozdemir","year":"2017","journal-title":"J Infect Dev Ctries"},{"issue":"5","key":"pone.0329326.ref033","doi-asserted-by":"crossref","first-page":"1864","DOI":"10.1542\/peds.2006-1091","article-title":"Impact of umbilical cord cleansing with 4.0% chlorhexidine on time to cord separation among newborns in southern Nepal: a cluster-randomized, community-based trial","volume":"118","author":"LC Mullany","year":"2006","journal-title":"Pediatrics"},{"issue":"2","key":"pone.0329326.ref034","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/0167-5877(86)90019-X","article-title":"Dairy calf management, morbidity and mortality in Ontario Holstein herds. III. Association of management with morbidity","volume":"4","author":"D Waltner-Toews","year":"1986","journal-title":"Preventive Vet Med"},{"key":"pone.0329326.ref035","article-title":"The effect of umbilical cord cleansing with chlorhexidine on omphalitis and neonatal mortality in community settings in developing countries: a meta-analysis","author":"A Imdad","year":"2013","journal-title":"BMC Public Health"},{"key":"pone.0329326.ref036","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.puhe.2016.05.006","article-title":"Neonatal mortality and topical application of chlorhexidine on umbilical cord stump: a meta-analysis of randomized control trials","volume":"139","author":"JA Shariff","year":"2016","journal-title":"Public Health"},{"issue":"1","key":"pone.0329326.ref037","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1097\/INF.0b013e3182783dc3","article-title":"Topical umbilical cord care for prevention of infection and neonatal mortality","volume":"32","author":"J Karumbi","year":"2013","journal-title":"Pediatr Infect Dis J"},{"key":"pone.0329326.ref038","unstructured":"PraeRi. Animal health, hygiene and biosecurity in German dairy cow operations \u2013 a prevalence study (PraeRi), final report. 2020. https:\/\/ibei.tiho-hannover.de\/praeri\/pages\/69#_AB"},{"issue":"9","key":"pone.0329326.ref039","doi-asserted-by":"crossref","first-page":"1385","DOI":"10.3390\/ani14091385","article-title":"Application of Epidemiological Methods in a Large-Scale Cross-Sectional Study in 765 German Dairy Herds-Lessons Learned","volume":"14","author":"R Merle","year":"2024","journal-title":"Animals (Basel)"},{"issue":"8","key":"pone.0329326.ref040","doi-asserted-by":"crossref","first-page":"9037","DOI":"10.3168\/jds.2021-20283","article-title":"Prevalence of disorders in preweaned dairy calves from 731 dairies in Germany: A cross-sectional study","volume":"104","author":"L Dachrodt","year":"2021","journal-title":"J Dairy Sci"},{"issue":"6","key":"pone.0329326.ref041","first-page":"1887","article-title":"Robust causal inference using directed acyclic graphs: the R package \u201cdagitty\u201d","volume":"45","author":"J Textor","year":"2016","journal-title":"Int J Epidemiol"},{"issue":"9","key":"pone.0329326.ref042","doi-asserted-by":"crossref","first-page":"7654","DOI":"10.3168\/jds.2021-21666","article-title":"Navel healing in male and female Holstein calves over the first 14 days of life: A longitudinal cohort study","volume":"105","author":"TE von Konigslow","year":"2022","journal-title":"J Dairy Sci"},{"issue":"3","key":"pone.0329326.ref043","doi-asserted-by":"crossref","first-page":"2659","DOI":"10.3168\/jds.2017-13581","article-title":"Risk factors associated with mortality at a milk-fed veal calf facility: A prospective cohort study","volume":"101","author":"DL Renaud","year":"2018","journal-title":"J Dairy Sci"},{"issue":"10","key":"pone.0329326.ref044","doi-asserted-by":"crossref","first-page":"9224","DOI":"10.3168\/jds.2019-16829","article-title":"Risk factors identified on arrival associated with morbidity and mortality at a grain-fed veal facility: A prospective, single-cohort study","volume":"102","author":"K Scott","year":"2019","journal-title":"J Dairy Sci"},{"key":"pone.0329326.ref045","doi-asserted-by":"crossref","unstructured":"Mee JF. 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In: Proceedings of the Forty-First Annual Conference, American Association of Bovine Practitioners, 2008. 46\u201353.","DOI":"10.21423\/aabppro20084365"},{"issue":"12","key":"pone.0329326.ref046","first-page":"1265","article-title":"Risk factors for poor health outcomes for male dairy calves undergoing transportation in western Canada","volume":"61","author":"DJ Wilson","year":"2020","journal-title":"Can Vet J"},{"issue":"7","key":"pone.0329326.ref047","doi-asserted-by":"crossref","first-page":"1413","DOI":"10.1017\/S1751731117003160","article-title":"Analysis of pre-weaning feeding policies and other risk factors influencing growth rates in calves on 11 commercial dairy farms","volume":"12","author":"KF Johnson","year":"2018","journal-title":"Animal"},{"issue":"5","key":"pone.0329326.ref048","doi-asserted-by":"crossref","first-page":"2669","DOI":"10.2903\/j.efsa.2012.2669","article-title":"Scientific Opinion on the welfare of cattle kept for beef production and the welfare in intensive calf farming systems","volume":"10","author":"EFSA Panel on Animal Health and Welfare (AHAW)","year":"2012","journal-title":"EFSA J"},{"issue":"10","key":"pone.0329326.ref049","doi-asserted-by":"crossref","first-page":"9418","DOI":"10.3168\/jds.2020-18177","article-title":"A survey of management practices that influence calf welfare and an estimation of the annual calf mortality risk in pastured dairy herds in Uruguay","volume":"103","author":"CO Schild","year":"2020","journal-title":"J Dairy Sci"},{"key":"pone.0329326.ref050","first-page":"70","article-title":"Efficacy of a new navel dip to prevent umbilical infection in dairy calves","volume":"45","author":"WM Grover","year":"2011","journal-title":"Am Assoc Bovine Practitioners"},{"key":"pone.0329326.ref051","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/0301-6226(90)90043-6","article-title":"Management factors related to calf morbidity and mortality rates","volume":"25","author":"EN Perez","year":"1990","journal-title":"Livestock Production Science"},{"issue":"8","key":"pone.0329326.ref052","doi-asserted-by":"crossref","first-page":"5110","DOI":"10.3168\/jds.2013-7695","article-title":"Farm characteristics and calf management practices on dairy farms with and without diarrhea: a case-control study to investigate risk factors for calf diarrhea","volume":"97","author":"D Klein-J\u00f6bstl","year":"2014","journal-title":"J Dairy Sci"},{"issue":"11","key":"pone.0329326.ref053","doi-asserted-by":"crossref","first-page":"9186","DOI":"10.3168\/jds.2017-13198","article-title":"Mortality and health treatment rates of dairy calves in automated milk feeding systems in the Upper Midwest of the United States","volume":"100","author":"MW Jorgensen","year":"2017","journal-title":"J Dairy Sci"},{"issue":"4","key":"pone.0329326.ref054","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1080\/00480169.2022.2042413","article-title":"A survey of calf rearing practices in the south-west region of Western Australia","volume":"70","author":"JW Aleri","year":"2022","journal-title":"N Z Vet J"},{"issue":"7","key":"pone.0329326.ref055","doi-asserted-by":"crossref","first-page":"6083","DOI":"10.3168\/jds.2021-21418","article-title":"Evaluating the effectiveness of a single application of 7% iodine tincture umbilical dip as a prevention of infection of the external umbilical structures in dairy calves","volume":"105","author":"MB Van Camp","year":"2022","journal-title":"J Dairy Sci"},{"issue":"2","key":"pone.0329326.ref056","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.prevetmed.2013.10.019","article-title":"Factors associated with morbidity, mortality, and growth of dairy heifer calves up to 3 months of age","volume":"113","author":"MC Windeyer","year":"2014","journal-title":"Prev Vet Med"},{"key":"pone.0329326.ref057","first-page":"505","article-title":"Umbilical masses in calves: Diagnosis, treatment and complications","volume":"11","author":"GM Baxter","year":"1989","journal-title":"Compend Contin Educ Pract Vet"},{"issue":"1","key":"pone.0329326.ref058","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1186\/s13028-015-0134-y","article-title":"Results of an online questionnaire to survey calf management practices on dairy cattle breeding farms in Austria and to estimate differences in disease incidences depending on farm structure and management practices","volume":"57","author":"D Klein-J\u00f6bstl","year":"2015","journal-title":"Acta Vet Scand"},{"issue":"3","key":"pone.0329326.ref059","first-page":"157","article-title":"Impact of umbilical disinfection on the calf\u2019s umbilical health - A critical review of the literature","volume":"50","author":"D Lang","year":"2022","journal-title":"Tierarztl Prax Ausg G Grosstiere Nutztiere"},{"key":"pone.0329326.ref060","volume-title":"Auswirkungen verschiedener Nabeldesinfektionsmittel auf die klinische und sonographische Entwicklung des Nabels beim neonaten Fohlen","author":"J. Biermann","year":"2008"},{"key":"pone.0329326.ref061","article-title":"Comparison of a novel umbilical dip, Super7 (TM) Navel Dip, to 7% tincture of iodine to desiccate the umbilical remnant in neonatal Holstein dairy calves","author":"JR Gard","year":"2014"},{"issue":"22","key":"pone.0329326.ref062","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1136\/vr.103999","article-title":"Umbilical infections in foals: microbiological investigation and management","volume":"180","author":"E Rampacci","year":"2017","journal-title":"Vet Rec"},{"issue":"8","key":"pone.0329326.ref063","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pmed.0020232","article-title":"Agricultural antibiotics and human health","volume":"2","author":"DL Smith","year":"2005","journal-title":"PLoS Med"},{"issue":"1","key":"pone.0329326.ref064","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1111\/zph.13186","article-title":"Characterisation of antimicrobial resistance in special-fed veal production environments","volume":"72","author":"A Dunmyre","year":"2025","journal-title":"Zoonoses Public Health"},{"key":"pone.0329326.ref065","volume-title":"WHO recommendations on maternal and newborn care for a positive postnatal experience","author":"World Health Organization","year":"2022"},{"issue":"1","key":"pone.0329326.ref066","doi-asserted-by":"crossref","first-page":"513","DOI":"10.3168\/jds.2016-11654","article-title":"The influence of 3 different navel dips on calf health, growth performance, and umbilical infection assessed by clinical and ultrasonographic examination","volume":"100","author":"M Wieland","year":"2017","journal-title":"J Dairy Sci"},{"issue":"10","key":"pone.0329326.ref067","doi-asserted-by":"crossref","DOI":"10.1002\/vetr.2730","article-title":"Influence of birth weight, sex and disinfection on the involution of umbilical structures in calves","volume":"192","author":"D Lang","year":"2023","journal-title":"Vet Rec"},{"key":"pone.0329326.ref068","first-page":"175","author":"SM McGuirk","year":"2011","journal-title":"Management of Dairy Calves from Birth to Weaning. Dairy Production Medicine"},{"issue":"6","key":"pone.0329326.ref069","doi-asserted-by":"crossref","first-page":"3973","DOI":"10.3168\/jds.2013-7325","article-title":"Survey of preweaning dairy calf-rearing practices in Czech dairy herds","volume":"97","author":"S Stan\u011bk","year":"2014","journal-title":"J Dairy Sci"},{"issue":"6","key":"pone.0329326.ref070","doi-asserted-by":"crossref","first-page":"7039","DOI":"10.3168\/jds.2020-19829","article-title":"Implementation of management recommendations in unweaned dairy calves in western Germany and associated challenges","volume":"104","author":"JJ Hayer","year":"2021","journal-title":"J Dairy Sci"},{"issue":"14","key":"pone.0329326.ref071","doi-asserted-by":"crossref","first-page":"1655","DOI":"10.1080\/14767058.2016.1220530","article-title":"Umbilical cord care: national survey, literature review and recommendations","volume":"30","author":"G Nosan","year":"2017","journal-title":"J Matern Fetal Neonatal Med"},{"issue":"3","key":"pone.0329326.ref072","doi-asserted-by":"crossref","first-page":"268","DOI":"10.3109\/01480545.2013.846364","article-title":"Povidone-iodine-induced cell death in cultured human epithelial HeLa cells and rat oral mucosal tissue","volume":"37","author":"S Sato","year":"2014","journal-title":"Drug Chem Toxicol"},{"key":"pone.0329326.ref073","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1159\/000090102","article-title":"Comparative study of in vitro cytotoxicity of povidone-iodine in solution, in ointment or in a liposomal formulation (Repithel) and selected antiseptics","author":"G M\u00fcller","year":"2006","journal-title":"Dermatology"},{"key":"pone.0329326.ref074","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.ijsu.2017.06.073","article-title":"Povidone iodine in wound healing: A review of current concepts and practices","volume":"44","author":"PL Bigliardi","year":"2017","journal-title":"Int J Surg"},{"issue":"3","key":"pone.0329326.ref075","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1001\/archsurg.1980.01380030009003","article-title":"Disinfection of surgical wounds without inhibition of normal wound healing","volume":"115","author":"J Viljanto","year":"1980","journal-title":"Arch Surg"},{"issue":"4","key":"pone.0329326.ref076","doi-asserted-by":"crossref","first-page":"869","DOI":"10.14202\/vetworld.2023.869-881","article-title":"Staphylococcus aureus, Escherichia coli, and Klebsiella spp. prevalence in bulk tank milk of Colombian herds and associated milking practices","volume":"16","author":"\u00c1S \u00c1gredo-Campos","year":"2023","journal-title":"Vet World"},{"issue":"5","key":"pone.0329326.ref077","doi-asserted-by":"crossref","first-page":"3839","DOI":"10.3168\/jds.2016-12187","article-title":"The Canadian National Dairy Study 2015-Adoption of milking practices in Canadian dairy herds","volume":"100","author":"E Belage","year":"2017","journal-title":"J Dairy Sci"},{"issue":"3","key":"pone.0329326.ref078","doi-asserted-by":"crossref","first-page":"1283","DOI":"10.3168\/jds.2011-4711","article-title":"Manageable risk factors associated with the lactational incidence, elimination, and prevalence of Staphylococcus aureus intramammary infections in dairy cows","volume":"95","author":"S Dufour","year":"2012","journal-title":"J Dairy Sci"},{"key":"pone.0329326.ref079","first-page":"77","volume-title":"Comparing bacterial counts on bare hands with gloved hands during milking Mastitis control From science to practice","author":"Olde Riekerink R.G.M. OCS, V.J. Eerland, M.J. Swarts, TJGM. 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