{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T00:21:52Z","timestamp":1758846112928,"version":"3.44.0"},"reference-count":38,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T00:00:00Z","timestamp":1758758400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Subsidies for Current Expenditures to Private Institutions of Higher Education from the Promotion and Mutual Aid Corporation for Private Schools of Japan"},{"name":"Dysautonomia International","award":["90097658"],"award-info":[{"award-number":["90097658"]}]},{"name":"Dysautonomia International","award":["90097658"],"award-info":[{"award-number":["90097658"]}]},{"name":"NIH","award":["R01NS126398"],"award-info":[{"award-number":["R01NS126398"]}]},{"name":"NIH","award":["R01NS126398"],"award-info":[{"award-number":["R01NS126398"]}]},{"DOI":"10.13039\/100029725","name":"Banks Family Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100029725","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Skalka-Kronsberg family"},{"name":"NIH","award":["T32CA126607"],"award-info":[{"award-number":["T32CA126607"]}]},{"name":"NIH","award":["T32GM136577"],"award-info":[{"award-number":["T32GM136577"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>Vascular access in mice is a cornerstone of biomedical research, with peripheral venous approaches like the lateral tail vein, retrobulbar venous sinus, facial vein, and saphenous vein being common. However, central venous approaches are challenging due to animal size and required expertise. To address this, we developed the Sternoclavicular joint-Targeted External jugular venipuncture Method (STEM). This technique provides reliable, longitudinal vascular access for frequent blood sampling using palpable surface anatomy landmarks. Moreover, STEM eliminates the need for fur shaving, specialized restraints, or deep sedation, allowing a single operator to perform the procedure safely and efficiently. Our protocol, based on a comprehensive anatomical analysis, revealed that the external jugular vein in mice traverses anteriorly to the clavicle before draining into the subclavian vein \u2013 a key anatomical difference from humans. This finding enabled a refined technique using the sternoclavicular joint as a landmark, improving the success and reproducibility of central venous access. Finally, STEM facilitates efficient blood collection and accurate intravenous administration with minimal setup time. It is straightforward and easily replicable, allowing researchers of all expertise levels to achieve high precision and reproducibility. The simplified learning process and consistent results make STEM valuable for various mouse-based experiments in biomedical research.<\/jats:p>","DOI":"10.1371\/journal.pone.0329811","type":"journal-article","created":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T17:30:29Z","timestamp":1758821429000},"page":"e0329811","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["A new external jugular venipuncture technique for efficient vascular access that exploits a murine anatomical 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Med"},{"issue":"3","key":"pone.0329811.ref007","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1258\/la.2008.007073","article-title":"Blood collection from the sublingual vein in mice and hamsters: a suitable alternative to retrobulbar technique that provides large volumes and minimizes tissue damage","volume":"43","author":"M Heimann","year":"2009","journal-title":"Lab Anim"},{"issue":"4","key":"pone.0329811.ref008","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1258\/002367798780599910","article-title":"Refinement of blood sampling from the sublingual vein of rats","volume":"32","author":"W Zeller","year":"1998","journal-title":"Lab Anim"},{"issue":"3","key":"pone.0329811.ref009","first-page":"8","article-title":"Evaluation of saphenous venipuncture and modified tail-clip blood collection in mice","volume":"47","author":"OI Abatan","year":"2008","journal-title":"J Am Assoc Lab Anim Sci"},{"issue":"4","key":"pone.0329811.ref010","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1258\/002367798780599866","article-title":"Saphenous vein puncture for blood sampling of the mouse, rat, hamster, gerbil, guinea pig, ferret and mink","volume":"32","author":"A Hem","year":"1998","journal-title":"Lab Anim"},{"issue":"1","key":"pone.0329811.ref011","first-page":"35","article-title":"Method to quantify tail vein injection technique in small animals","volume":"43","author":"EV Groman","year":"2004","journal-title":"Contemp Top Lab Anim Sci"},{"key":"pone.0329811.ref012","article-title":"Parenteral injections","author":"J Donovan","year":"2006","journal-title":"Curr Protoc Immunol"},{"issue":"1","key":"pone.0329811.ref013","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1002\/jat.727","article-title":"A good practice guide to the administration of substances and removal of blood, including routes and volumes","volume":"21","author":"KH Diehl","year":"2001","journal-title":"J Appl Toxicol"},{"issue":"3","key":"pone.0329811.ref014","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1258\/002367706777611433","article-title":"The impact of different blood sampling methods on laboratory rats under different types of anaesthesia","volume":"40","author":"M Fitzner Toft","year":"2006","journal-title":"Lab Anim"},{"issue":"2","key":"pone.0329811.ref015","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1258\/la.2010.010129","article-title":"Impact of blood sampling technique on blood quality and animal welfare in haemophilic mice","volume":"45","author":"H Holmberg","year":"2011","journal-title":"Lab Anim"},{"issue":"3","key":"pone.0329811.ref016","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1046\/j.1463-5224.2002.00251.x","article-title":"Ocular disease in rats: a review","volume":"5","author":"DL Williams","year":"2002","journal-title":"Vet Ophthalmol"},{"issue":"4","key":"pone.0329811.ref017","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1177\/0023677214541438","article-title":"Manual versus automated blood sampling: impact of repeated blood sampling on stress parameters and behavior in male NMRI mice","volume":"48","author":"AC Teilmann","year":"2014","journal-title":"Lab Anim"},{"issue":"1","key":"pone.0329811.ref018","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1177\/1535676019892068","article-title":"Development of improved devices for handling and restraining experimental laboratory mice","volume":"25","author":"M Aftalion","year":"2020","journal-title":"Appl Biosaf"},{"issue":"6","key":"pone.0329811.ref019","doi-asserted-by":"crossref","first-page":"445","DOI":"10.3109\/08941930903396412","article-title":"Guidelines for rodent survival surgery","volume":"22","author":"J Bernal","year":"2009","journal-title":"J Invest Surg"},{"issue":"47","key":"pone.0329811.ref020","first-page":"2586","article-title":"Principles of rodent surgery for the new surgeon","author":"KR Pritchett-Corning","year":"2011","journal-title":"J Vis Exp"},{"issue":"3071","key":"pone.0329811.ref021","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1126\/science.118.3071.563","article-title":"A jugular technique for the repeated bleeding of small animals","volume":"118","author":"R Kassel","year":"1953","journal-title":"Science"},{"issue":"3","key":"pone.0329811.ref022","first-page":"345","article-title":"Validation studies on blood collection from the jugular vein of conscious mice","volume":"51","author":"Y Shirasaki","year":"2012","journal-title":"J Am Assoc Lab Anim Sci"},{"key":"pone.0329811.ref023","unstructured":"M\u00fcller B. Blutentnahme aus dem retroorbitalen Venenplexus, den Schwanzvenen und dem jugularen Venenwinkel bei Ratte und Maus: vergleichende h\u00e4matologisch\/biochemische und klinische Untersuchungen. na. 1999."},{"issue":"8","key":"pone.0329811.ref024","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1038\/laban.738","article-title":"Effects of different blood collection methods on indicators of welfare in mice","volume":"44","author":"P-P Tsai","year":"2015","journal-title":"Lab Anim (NY)"},{"issue":"9","key":"pone.0329811.ref025","doi-asserted-by":"crossref","first-page":"1244","DOI":"10.1002\/jat.3635","article-title":"Establishment and validation of microsampling techniques in wild rodents for ecotoxicological research","volume":"38","author":"C Imholt","year":"2018","journal-title":"J Appl Toxicol"},{"key":"pone.0329811.ref026","article-title":"Methods of blood collection in the mouse","author":"J Hoff","year":"2000","journal-title":"Lab Animal"},{"issue":"147","key":"pone.0329811.ref027","doi-asserted-by":"crossref","DOI":"10.3791\/59556","article-title":"Blood collection through subclavian vein puncture in mice","author":"H Yang","year":"2019","journal-title":"J Vis Exp"},{"issue":"2","key":"pone.0329811.ref028","doi-asserted-by":"crossref","first-page":"278","DOI":"10.2144\/96202rr05","article-title":"Method for multiple portal vein infusions in mice: quantitation of adenovirus-mediated hepatic gene transfer","volume":"20","author":"MJ Vrancken Peeters","year":"1996","journal-title":"Biotechniques"},{"issue":"3","key":"pone.0329811.ref029","first-page":"328","article-title":"Percutaneous intravenous injection in neonatal mice","volume":"49","author":"MS Sands","year":"1999","journal-title":"Lab Anim Sci"},{"issue":"5","key":"pone.0329811.ref030","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1038\/laban0511-155","article-title":"Retro-orbital injections in mice","volume":"40","author":"T Yardeni","year":"2011","journal-title":"Lab Anim (NY)"},{"issue":"93","key":"pone.0329811.ref031","doi-asserted-by":"crossref","DOI":"10.3791\/52037-v","article-title":"Intravenous injections in neonatal mice","author":"SE Gombash Lampe","year":"2014","journal-title":"J Vis Exp"},{"issue":"2","key":"pone.0329811.ref032","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1016\/j.jneumeth.2007.05.007","article-title":"Method to perform IV injections on mice using the facial vein","volume":"164","author":"HB Levene","year":"2007","journal-title":"J Neurosci Methods"},{"issue":"3","key":"pone.0329811.ref033","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1258\/002367703766453010","article-title":"Cannulation of the jugular vein in mice: a method for serial withdrawal of blood samples","volume":"37","author":"HA Bardelmeijer","year":"2003","journal-title":"Lab Anim"},{"issue":"1","key":"pone.0329811.ref034","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/0091-3057(79)90307-1","article-title":"Techniques for the chronic cannulation of the jugular vein in mice","volume":"11","author":"JE Barr","year":"1979","journal-title":"Pharmacol Biochem Behav"},{"issue":"5","key":"pone.0329811.ref035","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1016\/0031-9384(93)90298-T","article-title":"Chronic vascular catheterization in the mouse","volume":"54","author":"A Mokhtarian","year":"1993","journal-title":"Physiol Behav"},{"issue":"1","key":"pone.0329811.ref036","article-title":"The ARRIVE guidelines 2.0: updated guidelines for reporting animal research","volume":"4","author":"N Percie du Sert","year":"2020","journal-title":"BMJ Open Sci"},{"key":"pone.0329811.ref037","unstructured":"Council NR. Guide for the care and use of laboratory animals. 2010."},{"issue":"26","key":"pone.0329811.ref038","doi-asserted-by":"crossref","first-page":"1735","DOI":"10.1056\/NEJM199412293312602","article-title":"Complications and failures of subclavian-vein catheterization","volume":"331","author":"PF Mansfield","year":"1994","journal-title":"N Engl J Med"}],"container-title":["PLOS One"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0329811","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T17:30:44Z","timestamp":1758821444000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0329811"}},"subtitle":[],"editor":[{"given":"Erik","family":"Su","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2025,9,25]]},"references-count":38,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2025,9,25]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0329811","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,25]]}}}