{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T20:20:44Z","timestamp":1772223644465,"version":"3.50.1"},"reference-count":56,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T00:00:00Z","timestamp":1757894400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["AG080092-01"],"award-info":[{"award-number":["AG080092-01"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Alzheimer\u2019s disease is the most common form of dementia, and multiple lines of evidence support the relevance of A\u03b2 deposition and amyloid plaque accumulation in the neurotoxicity and cognitive decline in AD. Rare mutations in angiotensin-converting-enzyme-1 have been highly associated with late onset AD patients; however, the mechanism for ACE1 mutation in AD pathogenesis is unknown. While numerous studies have shown that ACE1 indeed catabolizes A\u03b2, majority of these studies were performed\n                    <jats:italic>in vitro,<\/jats:italic>\n                    and conflicting results have been reported in clinical and\n                    <jats:italic>in vivo<\/jats:italic>\n                    systems. Therefore, we further investigated this\n                    <jats:italic>in vivo<\/jats:italic>\n                    by generating and examining a novel mouse model. Specifically, we analyzed 6-month-old 5XFAD mice with ACE1 knockdown restricted to excitatory neurons, achieved by driving Cre recombinase expression under the CamKII\u03b1 promoter. These mice were generated by crossing 5XFAD mice to ACE1 conditional knockout mice expressing Cre specifically in excitatory neurons. Our analyses revealed that neuronal ACE1 knockdown does not significantly affect amyloid plaque load and neuroinflammation in the hippocampus and cortex of 5XFAD mice at 6-months of age.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0330193","type":"journal-article","created":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T17:25:11Z","timestamp":1757957111000},"page":"e0330193","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["ACE1 does not influence cerebral A\u03b2 degradation or amyloid plaque accumulation in 5XFAD mice"],"prefix":"10.1371","volume":"20","author":[{"given":"Sohee","family":"Jeon","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-1346-7437","authenticated-orcid":true,"given":"Alia 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