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Despite the availability of treatment options, remission rates for SAD remain low, highlighting the need for more effective interventions. To support the development of more effective therapies, a better understanding of the psychophysiological mechanisms underlying SAD is needed. This pilot study investigates whether anticipatory anxiety before a social interaction can be detected by multiple biosignals, with the aim of identifying potential biomarkers for SAD. Using a modified version of the Internet-based Stress Test for Social Anxiety Disorder, we measured physiological responses of 17 healthy volunteers, including heart rate, respiratory rate, electrodermal activity, head movements, and electroencephalography power across various frequencies. We found that anticipatory anxiety was associated with increased heart rate, respiratory rate, tonic EDA, and variance in head roll, alongside elevated theta, beta, and gamma power in EEG. Our results suggest that a combination of these biosignals may provide valuable insight into the psychophysiology of social anxiety, which could be useful for both mechanistic research and clinical applications. Future research should explore the role of these signals in clinical populations and during acute threat conditions to refine diagnostic and prognostic tools for SAD.<\/jats:p>","DOI":"10.1371\/journal.pone.0330603","type":"journal-article","created":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T17:53:56Z","timestamp":1757008436000},"page":"e0330603","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Detection of social anxiety using multiple simultaneous biosignals: A pilot study"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0324-6626","authenticated-orcid":true,"given":"Christoph","family":"Tremmel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathan T. 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severity","volume":"8","author":"GA Alvares","year":"2013","journal-title":"PLoS One."},{"issue":"1","key":"pone.0330603.ref016","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.ijpsycho.2012.10.012","article-title":"Heart rate and heart rate variability in panic, social anxiety, obsessive-compulsive, and generalized anxiety disorders at baseline and in response to relaxation and hyperventilation","volume":"87","author":"A Pittig","year":"2013","journal-title":"Int J Psychophysiol."},{"key":"pone.0330603.ref017","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.janxdis.2018.07.001","article-title":"Children with social anxiety disorder show blunted pupillary reactivity and altered eye contact processing in response to emotional faces: Insights from pupillometry and eye movements","volume":"58","author":"V Keil","year":"2018","journal-title":"J Anxiety Disord."},{"issue":"7","key":"pone.0330603.ref018","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1007\/s11920-019-1036-x","article-title":"Psychophysiological markers of fear and anxiety","volume":"21","author":"J Hyde","year":"2019","journal-title":"Curr Psychiatry Rep."},{"key":"pone.0330603.ref019","doi-asserted-by":"crossref","first-page":"80","DOI":"10.3389\/fpsyt.2014.00080","article-title":"Anxiety disorders are associated with reduced heart rate variability: a meta-analysis","volume":"5","author":"JA Chalmers","year":"2014","journal-title":"Front Psychiatry."},{"key":"pone.0330603.ref020","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.bbr.2017.12.021","article-title":"Psychological stress level detection based on electrodermal activity","volume":"341","author":"Y Liu","year":"2018","journal-title":"Behav Brain 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Pain."},{"issue":"2","key":"pone.0330603.ref024","doi-asserted-by":"crossref","first-page":"155","DOI":"10.3390\/pr8020155","article-title":"Detection of drivers\u2019 anxiety invoked by driving situations using multimodal biosignals","volume":"8","author":"S Lee","year":"2020","journal-title":"Processes."},{"issue":"10","key":"pone.0330603.ref025","doi-asserted-by":"crossref","first-page":"3499","DOI":"10.3390\/s21103499","article-title":"The concept of advanced multi-sensor monitoring of human stress","volume":"21","author":"E Vavrinsky","year":"2021","journal-title":"Sensors (Basel)."},{"key":"pone.0330603.ref026","unstructured":"LabStreamingLayer. https:\/\/github.com\/sccn\/labstreaminglayer."},{"key":"pone.0330603.ref027","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1097\/00004691-199104000-00007","article-title":"American electroencephalographic society guidelines for standard electrode position nomenclature","volume":"8","author":"F Sharbrough","year":"1991","journal-title":"Clin Neurophysiol."},{"key":"pone.0330603.ref028","doi-asserted-by":"crossref","first-page":"114770","DOI":"10.1016\/j.psychres.2022.114770","article-title":"A novel procedure to investigate social anxiety using videoconferencing software: a proof-of-concept study","volume":"316","author":"NTM Huneke","year":"2022","journal-title":"Psychiatry Res."},{"issue":"10","key":"pone.0330603.ref029","doi-asserted-by":"crossref","first-page":"1092","DOI":"10.1001\/archinte.166.10.1092","article-title":"A brief measure for assessing generalized anxiety disorder: the GAD-7","volume":"166","author":"RL Spitzer","year":"2006","journal-title":"Arch Intern Med."},{"issue":"4","key":"pone.0330603.ref030","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1093\/ijnp\/pyaa019","article-title":"Brain functional connectivity correlates of response in the 7.5% CO2 inhalational model of generalized anxiety disorder: a pilot study","volume":"23","author":"NTM Huneke","year":"2020","journal-title":"Int J Neuropsychopharmacol."},{"issue":"1","key":"pone.0330603.ref031","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/S0987-7053(00)00055-1","article-title":"Removal of ocular artifact from the EEG: a review","volume":"30","author":"RJ Croft","year":"2000","journal-title":"Neurophysiol Clin."},{"issue":"3","key":"pone.0330603.ref032","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1016\/S0013-4694(97)00022-2","article-title":"Spatial filter selection for EEG-based communication","volume":"103","author":"DJ McFarland","year":"1997","journal-title":"Electroencephalogr Clin Neurophysiol."},{"issue":"1","key":"pone.0330603.ref033","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1469-8986.2008.00702.x","article-title":"Natural selective attention: orienting and emotion","volume":"46","author":"MM Bradley","year":"2009","journal-title":"Psychophysiology."},{"issue":"3","key":"pone.0330603.ref034","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/S0301-0511(01)00113-2","article-title":"Social anxiety and response to touch: incongruence between self-evaluative and physiological reactions","volume":"58","author":"FH Wilhelm","year":"2001","journal-title":"Biol Psychol."},{"issue":"2","key":"pone.0330603.ref035","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1177\/09567976211032231","article-title":"Physiological responses to a Haunted-House threat experience: distinct tonic and phasic effects","volume":"33","author":"SM Tashjian","year":"2022","journal-title":"Psychol Sci."},{"issue":"8","key":"pone.0330603.ref036","doi-asserted-by":"crossref","first-page":"1877","DOI":"10.1016\/j.clinph.2007.04.027","article-title":"Scalp electrical recording during paralysis: quantitative evidence that EEG frequencies above 20 Hz are contaminated by EMG","volume":"118","author":"EM Whitham","year":"2007","journal-title":"Clin Neurophysiol."},{"key":"pone.0330603.ref037","doi-asserted-by":"crossref","first-page":"101","DOI":"10.3389\/fncom.2016.00101","article-title":"Stress assessment by prefrontal relative gamma","volume":"10","author":"J Minguillon","year":"2016","journal-title":"Front Comput Neurosci."},{"issue":"6","key":"pone.0330603.ref038","doi-asserted-by":"crossref","first-page":"066022","DOI":"10.1088\/1741-2552\/ad8ef8","article-title":"Estimating cognitive workload using a commercial in-ear EEG headset","volume":"21","author":"C Tremmel","year":"2024","journal-title":"J Neural Eng."},{"key":"pone.0330603.ref039","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/j.nicl.2017.11.010","article-title":"Behavioral and EEG responses to social evaluation: a two-generation family study on social anxiety","volume":"17","author":"A Harrewijn","year":"2017","journal-title":"Neuroimage Clin."},{"key":"pone.0330603.ref040","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.3389\/fneur.2018.01029","article-title":"Arousal effects on pupil size, heart rate, and skin conductance in an emotional face task","volume":"9","author":"C-A Wang","year":"2018","journal-title":"Front Neurol."},{"key":"pone.0330603.ref041","unstructured":"Ciresi S, Hewitt J, Raterink C. 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