{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T03:56:27Z","timestamp":1793246187663,"version":"4.5.0"},"reference-count":70,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T00:00:00Z","timestamp":1757894400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"ERA-NET EMIDA"},{"DOI":"10.13039\/501100000268","name":"UK Biotechnology and Biological Sciences Research Council","doi-asserted-by":"crossref","award":["BB\/1017240\/1"],"award-info":[{"award-number":["BB\/1017240\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>The bovine endometrium undergoes cellular, molecular, and functional changes to support embryo survival and development to term. These changes involve a finely coordinated series of events at both local and systemic levels. We postulated that circulating white blood cells (WBCs) could provide valuable biomarkers for predicting pregnancy success in heifers undergoing embryo transfer, when, sampled during both a preconception cycle and a conception cycle, before embryo transfer takes place. WBCs were isolated using PAXgene Blood RNA tubes collected from Holstein-Friesian heifers on Days 7 and 14 of a preconception estrous cycle (PCD7 and PCD14) and, after a rest cycle, on Day 7 of the subsequent estrous cycle (1ETD7) just before embryo transfer. Circulating progesterone and estrogens were assayed and pregnancy was confirmed by either uterine flushing and conceptus collection on Day 18 post-estrus, or the delivery of a healthy calf. Using a custom bovine gene expression microarray representing 19,479 unique transcript, comparison of transcriptomes between heifers classified as non-pregnant or pregnant revealed 1,240, 896 and 1,023 differentially expressed genes (DEGs) at PCD7, PCD14 and 1ETD7 respectively. Our bioinformatics analyses revealed that pregnancy failure after embryo transfer was associated with upstream regulators, biological functions, canonical pathways and gene networks related to inflammation, immunity, apoptosis and cell death regulation, cell proliferation, membrane compounds, lipid metabolism, oxygen transport and ions transport. The heifers classified as non-pregnant showed significant increased transcripts levels of <jats:italic>PTGR1<\/jats:italic> at the three time points (PCD7, PCD14 and 1ETD7), <jats:italic>AIF1<\/jats:italic> at PCD14, <jats:italic>FNDC3B<\/jats:italic>, <jats:italic>IL15<\/jats:italic> and <jats:italic>SERPINE1<\/jats:italic> at 1ETD7. Our findings highlight the potential of peripheral WBCs as a non-invasive source of biomarkers for predicting pregnancy outcomes, offering promising insights for improving pregnancy success when reproductive biotechnologies are used in mammalian females.<\/jats:p>","DOI":"10.1371\/journal.pone.0330701","type":"journal-article","created":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T17:37:06Z","timestamp":1757957826000},"page":"e0330701","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Gene profiles of peripheral white blood cells as potential predictors of pregnancy in embryo-recipient heifers"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3828-1421","authenticated-orcid":true,"given":"Mariam","family":"Raliou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie 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