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The so-called \u201cplastisphere\u201d might facilitate the spread of pathogens and antimicrobial resistance across environments, posing risks to human and animal health. This study aimed to analyze the diversity and abundance of ARGs found in plastispheres from various aquatic environments, identify clinically relevant pathogenic species, and ascertain bacterial hosts carrying ARGs. We present data from 36 metagenomes collected from plastispheres in different environments (freshwater, raw wastewater, and treated wastewater). The diversity and abundance of ARGs in the resistome of the plastispheres were analyzed through metagenomic methods. A total of 537 high-quality metagenomic-assembled genomes (MAGs) were constructed to identify clinically relevant pathogens and to link the detected ARGs to their bacterial hosts. The results show that the environment has the greatest influence on the abundance and diversity of ARGs in the plastispheres resistome, with the wastewater plastisphere containing a resistome with the highest diversity of ARGs. Resistance to beta-lactams, aminoglycosides, and tetracyclines were the most abundant resistance mechanisms detected in the different plastispheres. The construction of MAGs identified potential pathogens and environmental bacteria that confer resistance to one or several drug classes, with beta-lactams being the most pervasive form of AMR detected. This work enhances our understanding of the plastisphere\u2019s role in antimicrobial resistance dissemination and its ecological and public health risks.<\/jats:p>","DOI":"10.1371\/journal.pone.0330754","type":"journal-article","created":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T17:54:46Z","timestamp":1756922086000},"page":"e0330754","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":13,"title":["Plastispheres as reservoirs of antimicrobial resistance: Insights from metagenomic analyses across aquatic environments"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6446-1768","authenticated-orcid":true,"given":"Ingun Lund","family":"Wits\u00f8","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amulya","family":"Baral","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann-Katrin","family":"Llarena","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Aspholm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mette","family":"Myrmel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yngvild","family":"Wasteson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,9,3]]},"reference":[{"key":"pone.0330754.ref001","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/B978-0-12-817880-5.00002-5","article-title":"Production, use, and fate of synthetic polymers","volume-title":"Plastic waste and recycling","author":"R Geyer","year":"2020"},{"key":"pone.0330754.ref002","first-page":"40","article-title":"Plastics\u2014The facts: An analysis of European latest plastics production, demand and waste data","volume":"1","author":"PlasticEurope","year":"2021","journal-title":"Plastics (Association Plast Manuf)"},{"issue":"6550","key":"pone.0330754.ref003","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1126\/science.abg5433","article-title":"The global threat from plastic pollution","volume":"373","author":"M MacLeod","year":"2021","journal-title":"Science"},{"key":"pone.0330754.ref004","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/j.watres.2017.07.005","article-title":"Solutions to microplastic pollution - 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