{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,23]],"date-time":"2025-08-23T00:08:33Z","timestamp":1755907713094,"version":"3.44.0"},"reference-count":55,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2025,8,22]],"date-time":"2025-08-22T00:00:00Z","timestamp":1755820800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004156","name":"Mahidol University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004156","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"\nPurpose<\/jats:title>\nTo investigate salivary microbiota composition in end-stage kidney disease (ESKD) patients with sarcopenia (SESKD), ESKD patients without sarcopenia (NSESKD), and individuals without chronic kidney disease (control group).<\/jats:p>\n<\/jats:sec>\n\nMaterials and Methods<\/jats:title>\nThirty-three participants were enrolled: 10 SESKD patients, 12 NSESKD patients, and 11 controls. Demographic data, oral examinations, and unstimulated saliva samples were collected. Salivary bacterial microbiomes were analyzed using high-throughput sequencing targeting the V3-V4 region of the bacterial 16S rRNA gene.<\/jats:p>\n<\/jats:sec>\n\nResults<\/jats:title>\nThe overall bacterial abundance and distribution were significantly higher in the SESKD and NSESKD groups compared to the control group (p<\/jats:italic><\/jats:italic>\u2009<\u20090.05), with no significant differences between the SESKD and NSESKD groups. ESKD, educational level, and muscle strength were significantly associated with variations in the salivary microbiome composition. Analysis of bacterial abundance revealed a shift in trends as the disease combined from control to NSESKD and SESKD group, respectively, across 7 genera: Actinobacillus<\/jats:italic><\/jats:italic>, TM7x<\/jats:italic>, Capnocytophaga<\/jats:italic><\/jats:italic>, Neisseria<\/jats:italic><\/jats:italic>, and Leptotrichia<\/jats:italic><\/jats:italic> increased in abundance, while Actinomyces<\/jats:italic><\/jats:italic> and Atopobium<\/jats:italic><\/jats:italic> decreased. Linear discriminant analysis effect size (LEfSe) identified Leptotrichia<\/jats:italic><\/jats:italic> as a potential biomarker for ESKD (both with and without sarcopenia).<\/jats:p>\n<\/jats:sec>\n\nConclusion<\/jats:title>\nESKD condition impacted microbial composition, with minimal influence from sarcopenia. Specifically, Leptotrichia<\/jats:italic><\/jats:italic> was notably higher in the ESKD group.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0330767","type":"journal-article","created":{"date-parts":[[2025,8,22]],"date-time":"2025-08-22T17:39:26Z","timestamp":1755884366000},"page":"e0330767","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Salivary microbiome in peritoneal dialysis patients with and without sarcopenia: A pilot 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