{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T07:26:49Z","timestamp":1792222009615,"version":"4.4.0"},"reference-count":47,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T00:00:00Z","timestamp":1757462400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>Luminal instruments are characterized by their slender internal lumens, which make them particularly challenging to clean and dry. A common drying method used by Sterile Processing Department (SPD) technicians involves blowing high-pressure air into one end of the lumen to expel moisture. However, this process generates a significant amount of aerosols that may contain bacteria, viruses, and other microorganisms. These aerosols can increase the microbial load in the SPD environment, leading to secondary contamination of other medical devices and posing health risks to SPD staff. Current methods used in SPD to reduce aerosols, such as gauze pockets and perforated boxes, have shown limited effectiveness.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods<\/jats:title>\n<jats:p>This study designed and fabricated a self-activating air filtration device, which primarily consists of four components: a centrifugal fan, a high-efficiency air filter, an acrylic box, and a diffuse reflective photoelectric switch. The total cost of the device is approximately $25. To verify the functionality of the device, we selected 20 sets of endoscopic instrument packs, 40 suction tubes, and 20 plastic hoses. The device\u2019s efficacy in reducing airborne dust particles and biological aerosols was evaluated, and a simple smoke test was conducted to assess its reliability.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Results<\/jats:title>\n<jats:p>Compared to results obtained without a HEPA filter, the self-activating air filtration device achieved a substantial reduction in airborne dust particles across various sizes: 79.34% for 0.3\u03bcm, 78.66% for 0.5\u03bcm, 76.58% for 1.0\u03bcm, 76.15% for 2.5\u03bcm, 72.55% for 5.0\u03bcm, and 75.00% for 10\u03bcm particles, with an average reduction of 76.36% (calculated using the median). No bacterial growth was observed on the ten culture plates sampled using the device, whereas five out of the ten culture plates sampled without the HEPA filter exhibited bacterial growth, resulting in a positivity rate of 50%. The differences were statistically significant. Additionally, the smoke test confirmed that the device effectively prevented contaminated air from escaping through the top opening of the acrylic box.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Conclusion<\/jats:title>\n<jats:p>The self-activating air filtration device significantly reduces the aerosols generated during the drying of luminal instruments, thereby mitigating the risks that aerosols pose to both the environment and staff health. Its automatic on-off functionality and low cost of use and maintenance make it a valuable solution for aerosol control in SPD and other hospital settings.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0331485","type":"journal-article","created":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T17:35:54Z","timestamp":1757525754000},"page":"e0331485","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Self-activating air filtration device for aerosol control during luminal instruments drying in the sterile processing department"],"prefix":"10.1371","volume":"20","author":[{"given":"Wei","family":"Zheng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Gui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-2889-8059","authenticated-orcid":true,"given":"Xiaoxue","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,9,10]]},"reference":[{"issue":"1","key":"pone.0331485.ref001","first-page":"33","article-title":"Optimization of a Sterile Processing Department Using Lean Six Sigma Methodology, Staffing Enhancement, and Capital Investment","volume":"51","author":"ME Natarus","year":"2025","journal-title":"Jt Comm J Qual Patient Saf"},{"issue":"3","key":"pone.0331485.ref002","doi-asserted-by":"crossref","first-page":"325","DOI":"10.3855\/jidc.18646","article-title":"Overview of the central sterilization supply department, an integral part of the hospital","volume":"19","author":"B Bal","year":"2025","journal-title":"J Infect Dev Ctries"},{"issue":"1","key":"pone.0331485.ref003","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1186\/s12913-024-12190-7","article-title":"Situations and demands of central sterile supply department training on nursing interruptions","volume":"25","author":"J Huang","year":"2025","journal-title":"BMC Health Serv Res"},{"issue":"10","key":"pone.0331485.ref004","first-page":"724","article-title":"Comparative study on the cleaning effect of two kinds of ATP fluorescence detection reagents on medical devices","volume":"37","author":"J Wang","year":"2020","journal-title":"Chin J Disinfect"},{"key":"pone.0331485.ref005","unstructured":"US Food and Drug Administration. 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Part 2: Standard for operating procedure of cleaning, disinfection and sterilization","author":"Health Commission of the People\u2019s Republic of China","year":"2016"},{"key":"pone.0331485.ref011","volume-title":"Decontamination and reprocessing of medical devices for health-care facilities","author":"World Health Organization, Pan American Health Organization","year":"2016"},{"key":"pone.0331485.ref012","volume-title":"ANSI\/AAMI ST79:2017 Comprehensive guide to steam sterilization and sterility assurance in health care facilities","author":"Association for the Advancement of Medical Instrumentation","year":"2017"},{"key":"pone.0331485.ref013","doi-asserted-by":"crossref","first-page":"43","DOI":"10.2147\/RMHP.S497773","article-title":"A Summary of the Best Evidence for Wet Pack Management","volume":"18","author":"L Yi","year":"2025","journal-title":"Risk Manag Healthc Policy"},{"key":"pone.0331485.ref014","article-title":"Analysis of Wet Pack Incidence in Steam Sterilization: A Study in a Chinese Medical Center","volume":"30","author":"Y Chen","year":"2024","journal-title":"Med Sci Monit"},{"issue":"2","key":"pone.0331485.ref015","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1177\/00494755231217322","article-title":"Updated review of wet pack complications in pulse vacuum pressure steam sterilisation processes in central sterile supply departments","volume":"54","author":"Q Huang","year":"2024","journal-title":"Trop Doct"},{"issue":"11","key":"pone.0331485.ref016","first-page":"1384","article-title":"Causes and countermeasures for failures in biological monitoring of hydrogen peroxide gas sterilization","volume":"22","author":"TT Cai","year":"2023","journal-title":"Chin J Infect Control"},{"issue":"5","key":"pone.0331485.ref017","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/j.ajic.2023.02.008","article-title":"How effective are the alcohol flush and drying cycles of automated endoscope reprocessors? 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Idei","year":"2020","journal-title":"Crit Care Explor"},{"issue":"22","key":"pone.0331485.ref040","first-page":"2868","article-title":"Application of the occupational protective bag in the drying procedure of hard endoscopes","volume":"18","author":"XY Chai","year":"2020","journal-title":"Chin Gen Pract Nurs"},{"issue":"11","key":"pone.0331485.ref041","first-page":"879","article-title":"Application of a high-pressure air gun noise and aerosol protective device","volume":"36","author":"DF Zhang","year":"2019","journal-title":"Chin J Disinfect"},{"issue":"11","key":"pone.0331485.ref042","first-page":"859","article-title":"Improving the protective functions of cleaning equipment in the decontamination area of the Central Sterile Supply Department","volume":"37","author":"DX Cao","year":"2020","journal-title":"Chin J Disinfect"},{"key":"pone.0331485.ref043","volume-title":"Hygienic standard for disinfection in hospitals","author":"General Administration of Quality Supervision & Inspection 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