{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T03:07:39Z","timestamp":1784862459405,"version":"3.55.0"},"reference-count":65,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T00:00:00Z","timestamp":1757289600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN-2016-06536"],"award-info":[{"award-number":["RGPIN-2016-06536"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["MOP133593"],"award-info":[{"award-number":["MOP133593"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Loss of actin cytoskeleton control can hinder integral developmental and physiological processes and can be the basis for a subset of developmental defects. SHROOM3 is an actin binding protein, best characterized as being essential for neural tube closure in vertebrates.\n                    <jats:italic>Shroom3<\/jats:italic>\n                    expression has also been identified in the developing heart, with some associated congenital heart defects. Here we show that the expression pattern of\n                    <jats:italic>Shroom3<\/jats:italic>\n                    in the developing and adult mouse heart is specific to the myocardium. Using a gene trap line, we show that embryos with homozygous full-body\n                    <jats:italic>Shroom3<\/jats:italic>\n                    loss die at birth due to exencephaly but also show congenital heart defects. This includes ventricular septal defects, semilunar valve abnormalities, and ventricle wall thinning. Adult mice heterozygous for\n                    <jats:italic>Shroom3<\/jats:italic>\n                    loss also show ventricular thinning due to decreased cardiomyocyte size. To explore if SHROOM3 is operating in a cell autonomous manner in the cardiomyocytes, we utilized a floxed\n                    <jats:italic>Shroom3<\/jats:italic>\n                    mouse line, allowing for spatial and temporal control of\n                    <jats:italic>Shroom3<\/jats:italic>\n                    loss. Using an Nkx2\u20135-Cre recombinase, we targeted\n                    <jats:italic>Shroom3<\/jats:italic>\n                    loss to the myocardium of the developing heart. Neonate pups with myocardial specific\n                    <jats:italic>Shroom3<\/jats:italic>\n                    loss showed no significant impact on heart development, including no septal or valve defects, no ventricular thinning, and no change in viability into adulthood. Adult mice with myocardial specific\n                    <jats:italic>Shroom3<\/jats:italic>\n                    loss showed no ventricular thinning and no change in cardiomyocyte size. These results show that the heart defects seen in full-body\n                    <jats:italic>Shroom3<\/jats:italic>\n                    loss do not arise from myocardial specific loss. Rather, other cell types expressing\n                    <jats:italic>Shroom3<\/jats:italic>\n                    , such as the cardiac neural crest cells, may be directly contributing to cardiac development.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0331583","type":"journal-article","created":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T17:45:52Z","timestamp":1757353552000},"page":"e0331583","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Understanding the role of Shroom3 in the developing mouse myocardium"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-7526-181X","authenticated-orcid":true,"given":"Jennifer L.","family":"Carleton","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rami R.","family":"Halabi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica A.","family":"Willson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy F.","family":"Plageman Jr.","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darren","family":"Bridgewater","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9739-2765","authenticated-orcid":true,"given":"Qingping","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas A.","family":"Drysdale","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,9,8]]},"reference":[{"issue":"21","key":"pone.0331583.ref001","doi-asserted-by":"crossref","DOI":"10.1161\/CIR.0000000000000606","article-title":"Genetic Basis for Congenital Heart Disease: Revisited: A Scientific Statement From the American Heart Association","volume":"138","author":"ME Pierpont","year":"2018","journal-title":"Circulation"},{"issue":"11","key":"pone.0331583.ref002","doi-asserted-by":"crossref","first-page":"962","DOI":"10.1002\/pd.5515","article-title":"The association between severe fetal congenital heart defects and placental vascular malperfusion lesions","volume":"39","author":"H Miremberg","year":"2019","journal-title":"Prenat Diagn"},{"issue":"1","key":"pone.0331583.ref003","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1097\/HCO.0000000000000816","article-title":"The placenta as the window to congenital heart disease","volume":"36","author":"JA Cohen","year":"2021","journal-title":"Curr Opin Cardiol"},{"issue":"12","key":"pone.0331583.ref004","doi-asserted-by":"crossref","first-page":"930","DOI":"10.1161\/CIRCULATIONAHA.122.060834","article-title":"Adults With Congenital Heart Disease: Trends in Event-Free Survival Past Middle Age","volume":"147","author":"M Dellborg","year":"2023","journal-title":"Circulation"},{"issue":"22","key":"pone.0331583.ref005","doi-asserted-by":"crossref","DOI":"10.1161\/JAHA.120.017704","article-title":"Survival in Children With Congenital Heart Disease: Have We Reached a Peak at 97%?","volume":"9","author":"Z Mandalenakis","year":"2020","journal-title":"J Am Heart Assoc"},{"issue":"2","key":"pone.0331583.ref006","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.ydbio.2020.05.013","article-title":"SHROOM3 is downstream of the planar cell polarity pathway and loss-of-function results in congenital heart defects","volume":"464","author":"MD Durbin","year":"2020","journal-title":"Dev Biol"},{"issue":"9","key":"pone.0331583.ref007","doi-asserted-by":"crossref","DOI":"10.1186\/gb-2011-12-9-r91","article-title":"SHROOM3 is a novel candidate for heterotaxy identified by whole exome sequencing","volume":"12","author":"M Tariq","year":"2011","journal-title":"Genome Biol"},{"issue":"8","key":"pone.0331583.ref008","doi-asserted-by":"crossref","first-page":"1369","DOI":"10.3390\/genes13081369","article-title":"Investigation of Genetic Causes in Patients with Congenital Heart Disease in Qatar: Findings from the Sidra Cardiac Registry","volume":"13","author":"S Okashah","year":"2022","journal-title":"Genes (Basel)"},{"issue":"5","key":"pone.0331583.ref009","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1016\/S0092-8674(00)81537-8","article-title":"Shroom, a PDZ domain-containing actin-binding protein, is required for neural tube morphogenesis in mice","volume":"99","author":"JD Hildebrand","year":"1999","journal-title":"Cell"},{"issue":"9","key":"pone.0331583.ref010","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1242\/bio.20147450","article-title":"The interaction between Shroom3 and Rho-kinase is required for neural tube morphogenesis in mice","volume":"3","author":"D Das","year":"2014","journal-title":"Biol Open"},{"key":"pone.0331583.ref011","doi-asserted-by":"crossref","first-page":"5191","DOI":"10.1242\/jcs.02626","article-title":"Shroom regulates epithelial cell shape via the apical positioning of an actomyosin network","volume":"118","author":"JD Hildebrand","year":"2005","journal-title":"J Cell Sci"},{"issue":"29","key":"pone.0331583.ref012","doi-asserted-by":"crossref","first-page":"20542","DOI":"10.1074\/jbc.M512463200","article-title":"Differential actin-dependent localization modulates the evolutionarily conserved activity of Shroom family proteins","volume":"281","author":"ML Dietz","year":"2006","journal-title":"J Biol Chem"},{"issue":"8","key":"pone.0331583.ref013","doi-asserted-by":"crossref","first-page":"1493","DOI":"10.1242\/dev.019646","article-title":"Shroom3-mediated recruitment of Rho kinases to the apical cell junctions regulates epithelial and neuroepithelial planar remodeling","volume":"135","author":"T Nishimura","year":"2008","journal-title":"Development"},{"issue":"2","key":"pone.0331583.ref014","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1242\/bio.20149589","article-title":"Shroom3 functions downstream of planar cell polarity to regulate myosin II distribution and cellular organization during neural tube closure","volume":"4","author":"EM McGreevy","year":"2015","journal-title":"Biol Open"},{"issue":"5","key":"pone.0331583.ref015","doi-asserted-by":"crossref","first-page":"1525","DOI":"10.1681\/ASN.2017080856","article-title":"Characterization of Coding\/Noncoding Variants for SHROOM3 in Patients with CKD","volume":"29","author":"JW Prokop","year":"2018","journal-title":"J Am Soc Nephrol"},{"issue":"24","key":"pone.0331583.ref016","doi-asserted-by":"crossref","first-page":"2125","DOI":"10.1016\/j.cub.2003.11.054","article-title":"Shroom induces apical constriction and is required for hingepoint formation during neural tube closure","volume":"13","author":"SL Haigo","year":"2003","journal-title":"Curr Biol"},{"issue":"8","key":"pone.0331583.ref017","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1242\/dev.044610","article-title":"Direct activation of Shroom3 transcription by Pitx proteins drives epithelial morphogenesis in the developing gut","volume":"137","author":"M-I Chung","year":"2010","journal-title":"Development"},{"issue":"10","key":"pone.0331583.ref018","doi-asserted-by":"crossref","first-page":"2965","DOI":"10.1681\/ASN.2015060621","article-title":"Developmental Origins for Kidney Disease Due to Shroom3 Deficiency","volume":"27","author":"H Khalili","year":"2016","journal-title":"J Am Soc Nephrol"},{"issue":"16","key":"pone.0331583.ref019","doi-asserted-by":"crossref","first-page":"3177","DOI":"10.1242\/dev.107433","article-title":"p120-catenin-dependent junctional recruitment of Shroom3 is required for apical constriction during lens pit morphogenesis","volume":"141","author":"RA Lang","year":"2014","journal-title":"Development"},{"issue":"7","key":"pone.0331583.ref020","doi-asserted-by":"crossref","first-page":"1431","DOI":"10.1242\/dev.02828","article-title":"Shroom family proteins regulate gamma-tubulin distribution and microtubule architecture during epithelial cell shape change","volume":"134","author":"C Lee","year":"2007","journal-title":"Development"},{"key":"pone.0331583.ref021","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.ydbio.2016.02.016","article-title":"Lens placode planar cell polarity is dependent on Cdc42-mediated junctional contraction inhibition","volume":"412","author":"M Muccioli","year":"2016","journal-title":"Dev Biol"},{"issue":"3","key":"pone.0331583.ref022","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1242\/dev.045369","article-title":"Pax6-dependent Shroom3 expression regulates apical constriction during lens placode invagination","volume":"137","author":"Jr Plageman TF","year":"2010","journal-title":"Development"},{"issue":"23","key":"pone.0331583.ref023","doi-asserted-by":"crossref","first-page":"5177","DOI":"10.1242\/dev.067868","article-title":"A Trio-RhoA-Shroom3 pathway is required for apical constriction and epithelial invagination","volume":"138","author":"Jr Plageman TF","year":"2011","journal-title":"Development"},{"issue":"1","key":"pone.0331583.ref024","doi-asserted-by":"crossref","first-page":"5400","DOI":"10.1038\/s41467-022-33115-0","article-title":"Optogenetic control of apical constriction induces synthetic morphogenesis in mammalian tissues","volume":"13","author":"G Mart\u00ednez-Ara","year":"2022","journal-title":"Nat Commun"},{"issue":"6","key":"pone.0331583.ref025","doi-asserted-by":"crossref","first-page":"1480","DOI":"10.1002\/dvdy.21942","article-title":"The shroom family proteins play broad roles in the morphogenesis of thickened epithelial sheets","volume":"238","author":"C Lee","year":"2009","journal-title":"Dev Dyn"},{"issue":"1","key":"pone.0331583.ref026","doi-asserted-by":"crossref","first-page":"51","DOI":"10.34067\/KID.0003802021","article-title":"Shroom3, a Gene Associated with CKD, Modulates Epithelial Recovery after AKI","volume":"3","author":"A Li","year":"2021","journal-title":"Kidney360"},{"issue":"1","key":"pone.0331583.ref027","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.ydbio.2011.06.027","article-title":"Shroom3 and a Pitx2-N-cadherin pathway function cooperatively to generate asymmetric cell shape changes during gut morphogenesis","volume":"357","author":"Jr Plageman TF","year":"2011","journal-title":"Dev Biol"},{"key":"pone.0331583.ref028","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.ydbio.2025.03.008","article-title":"Shroom3 facilitates optic fissure closure via tissue alignment and reestablishment of apical-basal polarity during epithelial fusion","volume":"522","author":"JA Herstine","year":"2025","journal-title":"Dev Biol"},{"issue":"3","key":"pone.0331583.ref029","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1111\/cge.13804","article-title":"A homozygous pathogenic variant in SHROOM3 associated with anencephaly and cleft lip and palate","volume":"98","author":"AR Deshwar","year":"2020","journal-title":"Clin Genet"},{"issue":"7","key":"pone.0331583.ref030","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1136\/jmedgenet-2015-103027","article-title":"Loss-of-function de novo mutations play an important role in severe human neural tube defects","volume":"52","author":"P Lemay","year":"2015","journal-title":"J Med Genet"},{"issue":"9","key":"pone.0331583.ref031","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1101\/gad.5.9.1513","article-title":"Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice","volume":"5","author":"G Friedrich","year":"1991","journal-title":"Genes Dev"},{"issue":"1","key":"pone.0331583.ref032","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1242\/dev.01562","article-title":"The Hand1 and Hand2 transcription factors regulate expansion of the embryonic cardiac ventricles in a gene dosage-dependent manner","volume":"132","author":"DG McFadden","year":"2005","journal-title":"Development"},{"issue":"5","key":"pone.0331583.ref033","doi-asserted-by":"crossref","DOI":"10.2144\/000112034","article-title":"Clearing and photography of whole mount X-gal stained mouse embryos","volume":"39","author":"O Schatz","year":"2005","journal-title":"Biotechniques"},{"key":"pone.0331583.ref034","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1016\/j.mod.2013.01.001","article-title":"Endothelial deletion of ADAM17 in mice results in defective remodeling of the semilunar valves and cardiac dysfunction in adults","volume":"130","author":"CL Wilson","year":"2013","journal-title":"Mech Dev"},{"issue":"1","key":"pone.0331583.ref035","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1242\/dev.126.1.75","article-title":"Control of early cardiac-specific transcription of Nkx2-5 by a GATA-dependent enhancer","volume":"126","author":"CL Lien","year":"1999","journal-title":"Development"},{"issue":"7705","key":"pone.0331583.ref036","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1038\/s41586-018-0110-6","article-title":"Control of cardiac jelly dynamics by NOTCH1 and NRG1 defines the building plan for trabeculation","volume":"557","author":"G Del Monte-Nieto","year":"2018","journal-title":"Nature"},{"issue":"1","key":"pone.0331583.ref037","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1002\/dvdy.552","article-title":"Development of ventricular trabeculae affects electrical conduction in the early endothermic heart","volume":"253","author":"V Olejnickova","year":"2024","journal-title":"Dev Dyn"},{"issue":"11","key":"pone.0331583.ref038","doi-asserted-by":"crossref","first-page":"105393","DOI":"10.1016\/j.isci.2022.105393","article-title":"Equal force generation potential of trabecular and compact wall ventricular cardiomyocytes","volume":"25","author":"JW Faber","year":"2022","journal-title":"iScience"},{"issue":"11","key":"pone.0331583.ref039","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1038\/s44161-022-00160-3","article-title":"Single-cell and spatial transcriptomics of the infarcted heart define the dynamic onset of the border zone in response to mechanical destabilization","volume":"1","author":"DM Calcagno","year":"2022","journal-title":"Nat Cardiovasc Res"},{"issue":"2","key":"pone.0331583.ref040","doi-asserted-by":"crossref","DOI":"10.1152\/ajpheart.00221.2005","article-title":"Beta-myosin heavy chain myocytes are more resistant to changes in power output induced by ischemic conditions","volume":"290","author":"AC Hinken","year":"2006","journal-title":"Am J Physiol Heart Circ Physiol"},{"issue":"19","key":"pone.0331583.ref041","doi-asserted-by":"crossref","first-page":"17466","DOI":"10.1074\/jbc.M210804200","article-title":"Analysis of myosin heavy chain functionality in the heart","volume":"278","author":"M Krenz","year":"2003","journal-title":"J Biol Chem"},{"issue":"12","key":"pone.0331583.ref042","doi-asserted-by":"crossref","first-page":"4019","DOI":"10.1113\/JP273123","article-title":"PITX2-dependent gene regulation in atrial fibrillation and rhythm control","volume":"595","author":"F Syeda","year":"2017","journal-title":"J Physiol"},{"issue":"12","key":"pone.0331583.ref043","article-title":"A cellular atlas of Pitx2-dependent cardiac development","volume":"146","author":"MC Hill","year":"2019","journal-title":"Development"},{"issue":"7","key":"pone.0331583.ref044","doi-asserted-by":"crossref","first-page":"1359","DOI":"10.1007\/s00246-019-02164-6","article-title":"Phases and Mechanisms of Embryonic Cardiomyocyte Proliferation and Ventricular Wall Morphogenesis","volume":"40","author":"Y Barak","year":"2019","journal-title":"Pediatr Cardiol"},{"issue":"4","key":"pone.0331583.ref045","first-page":"54","article-title":"Hemodynamics in Cardiac Development","volume":"5","author":"RE Poelmann","year":"2018","journal-title":"J Cardiovasc Dev Dis"},{"issue":"3","key":"pone.0331583.ref046","doi-asserted-by":"crossref","DOI":"10.1016\/j.devcel.2017.09.023","article-title":"Hemodynamic Forces Sculpt Developing Heart Valves through a KLF2-WNT9B Paracrine Signaling Axis","volume":"43","author":"LM Goddard","year":"2017","journal-title":"Dev Cell"},{"issue":"13","key":"pone.0331583.ref047","article-title":"Mechanisms of heart valve development and disease","volume":"147","author":"A O\u2019Donnell","year":"2020","journal-title":"Development"},{"issue":"8","key":"pone.0331583.ref048","first-page":"89","article-title":"The Cardiac Neural Crest Cells in Heart Development and Congenital Heart Defects","volume":"8","author":"S Erhardt","year":"2021","journal-title":"J Cardiovasc Dev Dis"},{"issue":"3","key":"pone.0331583.ref049","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1093\/cvr\/cvt132","article-title":"Neural crest cells are required for correct positioning of the developing outflow cushions and pattern the arterial valve leaflets","volume":"99","author":"HM Phillips","year":"2013","journal-title":"Cardiovasc Res"},{"key":"pone.0331583.ref050","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.47929","article-title":"Cardiac neural crest contributes to cardiomyocytes in amniotes and heart regeneration in zebrafish","volume":"8","author":"W Tang","year":"2019","journal-title":"Elife"},{"issue":"185","key":"pone.0331583.ref051","doi-asserted-by":"crossref","DOI":"10.3791\/64207","article-title":"En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos","author":"T Gonzalez-Costa","year":"2022","journal-title":"J Vis Exp"},{"issue":"9","key":"pone.0331583.ref052","article-title":"\u03b2-Catenin regulates endocardial cushion growth by suppressing p21","volume":"6","author":"H Liu","year":"2023","journal-title":"Life Sci Alliance"},{"issue":"1","key":"pone.0331583.ref053","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1002\/ar.a.10099","article-title":"Spatiotemporally separated cardiac neural crest subpopulations that target the outflow tract septum and pharyngeal arch arteries","volume":"275","author":"MJ Boot","year":"2003","journal-title":"Anat Rec A Discov Mol Cell Evol Biol"},{"issue":"3","key":"pone.0331583.ref054","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1002\/(SICI)1097-0177(199807)212:3<373::AID-AJA5>3.0.CO;2-E","article-title":"Neural crest cells in outflow tract septation of the embryonic chicken heart: Differentiation and apoptosis","volume":"212","author":"RE Poelmann","year":"1998","journal-title":"Dev Dyn"},{"issue":"2","key":"pone.0331583.ref055","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/S0008-6363(00)00127-9","article-title":"Cardiac neural crest: the holy grail of cardiac abnormalities?","volume":"47","author":"MJ van den Hoff","year":"2000","journal-title":"Cardiovasc Res"},{"key":"pone.0331583.ref056","doi-asserted-by":"crossref","first-page":"678954","DOI":"10.3389\/fcell.2021.678954","article-title":"Epigenetic Regulation of Cardiac Neural Crest Cells","volume":"9","author":"S Yan","year":"2021","journal-title":"Front Cell Dev Biol"},{"issue":"2","key":"pone.0331583.ref057","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1161\/01.RES.77.2.211","article-title":"Neural crest and cardiovascular patterning","volume":"77","author":"ML Kirby","year":"1995","journal-title":"Circ Res"},{"issue":"4","key":"pone.0331583.ref058","doi-asserted-by":"crossref","first-page":"609","DOI":"10.4161\/cam.4.4.13489","article-title":"Factors controlling cardiac neural crest cell migration","volume":"4","author":"ML Kirby","year":"2010","journal-title":"Cell Adh Migr"},{"issue":"20","key":"pone.0331583.ref059","doi-asserted-by":"crossref","DOI":"10.1242\/dev.188706","article-title":"The heart of the neural crest: cardiac neural crest cells in development and regeneration","volume":"147","author":"RM George","year":"2020","journal-title":"Development"},{"key":"pone.0331583.ref060","first-page":"589414","article-title":"Planar cell polarity signaling pathway in congenital heart diseases","volume":"2011","author":"G Wu","year":"2011","journal-title":"J Biomed Biotechnol"},{"issue":"5","key":"pone.0331583.ref061","doi-asserted-by":"crossref","DOI":"10.1161\/JAHA.114.001271","article-title":"Rac1 signaling is critical to cardiomyocyte polarity and embryonic heart development","volume":"3","author":"C Leung","year":"2014","journal-title":"J Am Heart Assoc"},{"issue":"1","key":"pone.0331583.ref062","doi-asserted-by":"crossref","first-page":"2161","DOI":"10.1038\/s41467-018-04566-1","article-title":"Planar cell polarity signalling coordinates heart tube remodelling through tissue-scale polarisation of actomyosin activity","volume":"9","author":"AM Merks","year":"2018","journal-title":"Nat Commun"},{"issue":"2","key":"pone.0331583.ref063","doi-asserted-by":"crossref","DOI":"10.1242\/dev.195008","article-title":"Neural tube closure requires the endocytic receptor Lrp2 and its functional interaction with intracellular scaffolds","volume":"148","author":"I Kowalczyk","year":"2021","journal-title":"Development"},{"key":"pone.0331583.ref064","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1186\/s12868-015-0171-5","article-title":"Targeted inhibition of the Shroom3-Rho kinase protein-protein interaction circumvents Nogo66 to promote axon outgrowth","volume":"16","author":"HM Dickson","year":"2015","journal-title":"BMC Neurosci"},{"issue":"11","key":"pone.0331583.ref065","article-title":"Pressure Overload Greatly Promotes Neonatal Right Ventricular Cardiomyocyte Proliferation: A New Model for the Study of Heart Regeneration","volume":"9","author":"L Ye","year":"2020","journal-title":"J Am Heart Assoc"}],"updated-by":[{"DOI":"10.1371\/journal.pone.0341029","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T00:00:00Z","timestamp":1768262400000}}],"container-title":["PLOS One"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0331583","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T23:09:53Z","timestamp":1766444993000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0331583"}},"subtitle":[],"editor":[{"given":"Federica","family":"Limana","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,9,8]]},"references-count":65,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2025,9,8]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0331583","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,8]]}}}