{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T00:31:40Z","timestamp":1760661100800,"version":"build-2065373602"},"reference-count":32,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:00:00Z","timestamp":1760572800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Joint Construction Project of Henan Medical Science and Technology Research Plan","award":["No. LHGJ20240461"],"award-info":[{"award-number":["No. LHGJ20240461"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>N6-methyladenosine (m6A) is a prevalent RNA modification in eukaryotes that regulates RNA stability and translation. Dysregulated m6A modification is implicated in cancer progression. This study investigated the role of the m6A reader protein, insulin-like growth factor 2 mRNA-binding protein 2 (IGF2 BP2), in the progression of thyroid cancer (TC).<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods<\/jats:title>\n<jats:p>Cell proliferation was assessed using cell counting kit-8 (CCK8) and 5-ethynyl-2\u2019-deoxyuridine (EdU) assays. Cell migration and invasion were evaluated by Transwell assays. A xenograft tumor model was employed to examine the impact of IGF2 BP2 on tumor growth <jats:italic><jats:italic>in vivo<\/jats:italic><\/jats:italic>. Gene functional annotation was performed through GO analysis. Spearman correlation analysis was utilized to evaluate the relationship between the expression levels of cathepsin H (CTSH) and IGF2 BP2. RIP-qPCR and RNA pull-down assays were conducted to confirm the interaction between IGF2 BP2 and CTSH mRNA.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Results<\/jats:title>\n<jats:p>Elevated IGF2 BP2 expression correlated significantly with advanced N stage in TC. Knockdown of IGF2 BP2 inhibited TC cell proliferation, migration, and invasion <jats:italic><jats:italic>in vitro<\/jats:italic><\/jats:italic>, as well as tumor growth <jats:italic><jats:italic>in vivo<\/jats:italic><\/jats:italic>. CTSH expression mirrored IGF2 BP2 expression. IGF2 BP2 interacted with CTSH mRNA, enhancing its stability in an m6A-dependent manner. Overexpression of CTSH counteracted the effects of IGF2 BP2 knockdown on TC cell proliferation, migration, invasion, and epithelial-to-mesenchymal transition (EMT).<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Conclusion<\/jats:title>\n<jats:p>IGF2 BP2 accelerates TC progression by recognizing and stabilizing m6A-modified CTSH mRNA. IGF2 BP2 and CTSH represent potential diagnostic and therapeutic targets for TC.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0332061","type":"journal-article","created":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T17:26:46Z","timestamp":1760635606000},"page":"e0332061","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["IGF2BP2 contributes to thyroid cancer progression by enhancing the stability of m6A-modified CTSH mRNA"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-5731-3239","authenticated-orcid":true,"given":"Liangpeng","family":"Dong","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingyun","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaofen","family":"Qi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiaochu","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Youwei","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengqi","family":"Hou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2025,10,16]]},"reference":[{"key":"pone.0332061.ref001","doi-asserted-by":"crossref","first-page":"102","DOI":"10.3389\/fendo.2020.00102","article-title":"Update 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