{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T16:06:08Z","timestamp":1774973168267,"version":"3.50.1"},"reference-count":43,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T00:00:00Z","timestamp":1758153600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82060442"],"award-info":[{"award-number":["82060442"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the Science and Technology Foundation of Guizhou Province","award":["[2025] General 126"],"award-info":[{"award-number":["[2025] General 126"]}]},{"name":"Key Laboratory for Cancer Prevention and treatment of Guizhou Province","award":["QKHPT [2025]031"],"award-info":[{"award-number":["QKHPT [2025]031"]}]},{"name":"Grant from Key Lab for Chronic Disease Biomarkers of Guizhou Medical University","award":["No.2024fy004"],"award-info":[{"award-number":["No.2024fy004"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>Background: In clinical practice, gastric cancer (GC) is a common malignancy with high morbidity. Accumulating research has revealed that lncRNAs are involved in the development and metastasis of tumor tissues in multiple cancers. As reported, <jats:italic>LINC00963<\/jats:italic>, a typical lncRNA is aberrantly expressed in gastric cancer. However, the underlying mechanisms of super-enhancers mediating remain unclear. Materials and methods: The GC cell line enhancer-super-enhancer data were downloaded and analyzed from the NCBI database (GSE75595). Combined RT-qPCR and Sanger sequencing were employed to identify three variants of <jats:italic>LINC00963<\/jats:italic> in gastric cell lines and peripheral blood samples from gastric cancer patients. Western blot was used to detect the expression level of epi-thelial-mesenchymal transition (EMT)-related proteins. Transwell assays were applied to assess the cell invasion and migration. A xenograft model was applied to simulate the tumor development process, during which the effect of <jats:italic>LINC00963<\/jats:italic> on promoting tu-morigenesis were investigated. Results: Analysis of the GC cell line enhanc-er-super-enhancer data revealed a high expression of <jats:italic>LINC00963<\/jats:italic> driven by su-per-enhancer. The variant 1 and variant 2 of <jats:italic>LINC00963<\/jats:italic> exhibited high expression in GC cell line and the peripheral blood of gastric cancers. <jats:italic>LINC00963<\/jats:italic> expression in the GC cell line was reduced after exposure to a low dose of the bromodomain and extraterminal inhibitor JQ1. Down-regulation of <jats:italic>LINC00963<\/jats:italic> variant 1 resulted in decreased levels of <jats:italic>\u03b2<\/jats:italic>-<jats:italic>catenin<\/jats:italic> and <jats:italic>ZEB1<\/jats:italic> proteins, and the protein expression levels of several marker proteins related to EMT, such as <jats:italic>Vimentin<\/jats:italic>, <jats:italic>N-cadherin<\/jats:italic> were observed to decrease (Fig 1). Conclusion: This study demonstrated that the super-enhancer-associated <jats:italic>LINC00963<\/jats:italic> promoting tumor metastasis in gastric cancer through EMT.<\/jats:p>","DOI":"10.1371\/journal.pone.0332396","type":"journal-article","created":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T17:43:31Z","timestamp":1758217411000},"page":"e0332396","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Super-enhancer-associated LINC00963 promotes metastasis of gastric cancer through epithelial-mesenchymal 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