{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T15:53:13Z","timestamp":1788537193739,"version":"build-2803163510"},"reference-count":36,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T00:00:00Z","timestamp":1758758400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82060271"],"award-info":[{"award-number":["82060271"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the Science Foundation of Guizhou Medical University","award":["22NSFCP46"],"award-info":[{"award-number":["22NSFCP46"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>Osteoarthritis (OA) and osteoporosis (OP) are prevalent conditions with overlapping molecular mechanisms. Recent studies have brought attention to ferroptosis, a type of cell death that relies on iron, as a possible connection between these diseases.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods<\/jats:title>\n<jats:p>We performed a comprehensive analysis with datasets obtained from the Gene Expression Omnibus (GEO), focusing on differentially expressed genes (DEGs) in OA and OP. Functional enrichment and integrated analyses identified ferroptosis-related pathways. The role of ferroptosis in OP was further explored through in vivo studies using an ovariectomy-induced OP mouse model.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Results<\/jats:title>\n<jats:p>The analysis revealed significant overlaps in DEGs related to ferroptosis pathways in both OA and OP. Key genes like TXNIP and SLC2A3 were implicated in the regulation of ferroptosis and associated with disease mechanisms. In vivo results confirmed increased ferroptosis markers in bone marrow stromal cells (BMSCs) from OP mice, supporting the hypothesis that ferroptosis contributes to bone density reduction and structural deterioration.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Conclusion<\/jats:title>\n<jats:p>Our findings highlight ferroptosis as a critical pathway in the pathogenesis of both OA and OP. Targeting ferroptosis-related genes and pathways could provide new therapeutic opportunities for managing these musculoskeletal diseases.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0332769","type":"journal-article","created":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T17:43:12Z","timestamp":1758822192000},"page":"e0332769","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Multimodal analysis investigating the shared pathogenic mechanisms of osteoporosis and osteoarthritis with an initial exploration of the role of ferroptosis"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-6459-8944","authenticated-orcid":true,"given":"Chunqing","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongcheng","family":"Ran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenyi","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weimin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,9,25]]},"reference":[{"issue":"2","key":"pone.0332769.ref001","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.cger.2021.11.005","article-title":"The burden of osteoarthritis in older adults","volume":"38","author":"GA Hawker","year":"2022","journal-title":"Clin Geriatr Med"},{"issue":"7","key":"pone.0332769.ref002","doi-asserted-by":"crossref","first-page":"1172","DOI":"10.1002\/art.42089","article-title":"Prevalence trends of site-specific osteoarthritis from 1990 to 2019: findings from the global burden of disease study 2019","volume":"74","author":"H Long","year":"2022","journal-title":"Arthritis Rheumatol"},{"issue":"4","key":"pone.0332769.ref003","doi-asserted-by":"crossref","DOI":"10.3390\/biomedicines11041220","article-title":"Physiopathology of osteoporosis: nursing involvement and management","volume":"11","author":"S Valenzuela-Mart\u00ednez","year":"2023","journal-title":"Biomedicines"},{"issue":"39","key":"pone.0332769.ref004","doi-asserted-by":"crossref","DOI":"10.1503\/cmaj.221647","article-title":"Clinical practice guideline for management of osteoporosis and fracture prevention in Canada: 2023 update","volume":"195","author":"SN Morin","year":"2023","journal-title":"CMAJ"},{"issue":"3","key":"pone.0332769.ref005","doi-asserted-by":"crossref","DOI":"10.3390\/nu14030523","article-title":"The role of macronutrients, micronutrients and flavonoid polyphenols in the prevention and treatment of osteoporosis","volume":"14","author":"M Martiniakova","year":"2022","journal-title":"Nutrients"},{"issue":"2","key":"pone.0332769.ref006","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1007\/s00431-022-04743-x","article-title":"Osteoporosis in children and adolescents: how to treat and monitor?","volume":"182","author":"S Ciancia","year":"2023","journal-title":"Eur J Pediatr"},{"key":"pone.0332769.ref007","doi-asserted-by":"crossref","first-page":"116068","DOI":"10.1016\/j.bone.2021.116068","article-title":"Osteoporosis, fracture, osteoarthritis & sarcopenia: A systematic review of circulating microRNA association","volume":"152","author":"TL Jones","year":"2021","journal-title":"Bone"},{"key":"pone.0332769.ref008","doi-asserted-by":"crossref","first-page":"1012508","DOI":"10.3389\/fendo.2022.1012508","article-title":"Osteopontin, a bridge links osteoarthritis and osteoporosis","volume":"13","author":"R-J Bai","year":"2022","journal-title":"Front Endocrinol (Lausanne)"},{"key":"pone.0332769.ref009","doi-asserted-by":"crossref","first-page":"1051134","DOI":"10.3389\/fphar.2022.1051134","article-title":"Extracellular vesicles derived from host and gut microbiota as promising nanocarriers for targeted therapy in osteoporosis and osteoarthritis","volume":"13","author":"KCP Cheung","year":"2023","journal-title":"Front Pharmacol"},{"key":"pone.0332769.ref010","doi-asserted-by":"crossref","first-page":"147914","DOI":"10.1016\/j.gene.2023.147914","article-title":"What do osteoporosis and osteoarthritis have in common? 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