{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T18:11:40Z","timestamp":1759947100094,"version":"build-2065373602"},"reference-count":28,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T00:00:00Z","timestamp":1759881600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Sysmex (Thailand) Co., Ltd.","award":["\u0e4cNot applicable"],"award-info":[{"award-number":["\u0e4cNot applicable"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>Flow cytometry remains the gold-standard method for enumerating CD34\u2009+\u2009cells during peripheral blood stem cell (PBSC) transplantation, but it is resource- and time-intensive. This study aimed to assess the correlation between hematopoietic progenitor cell (HPC) counts measured by a Sysmex XN-series analyzer and CD34\u2009+\u2009cell counts obtained by flow cytometry in pre-apheresis peripheral blood and apheresis products, and to establish HPC cutoffs for predicting adequate PBSC yields.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods<\/jats:title>\n<jats:p>We analyzed 334 samples (167 pre-apheresis peripheral blood, 167 PBSC apheresis products) collected from patients and healthy donors. HPC and CD34\u2009+\u2009cell counts were measured in all samples. Receiver operating characteristic (ROC) curve analysis was performed to determine HPC cutoff values predictive of adequate PBSC harvest.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Results<\/jats:title>\n<jats:p>Intraclass correlation coefficients (ICCs) indicated strong HPC\u2012CD34\u2009+\u2009concordance in pre-apheresis peripheral blood (ICC\u2009=\u20090.896, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) and apheresis products (ICC\u2009=\u20090.958, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). Median HPC counts were significantly higher than CD34\u2009+\u2009cell counts overall (16 vs. 14.6\/\u00b5L, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001; 1,040 vs. 804.73\/\u00b5L, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). The same trend was observed in multiple myeloma (50 vs. 23.92\/\u00b5L, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001; 1,290 vs. 1,132.85\/\u00b5L, <jats:italic>p<\/jats:italic>\u2009=\u20090.002) and lymphoma (7 vs. 2.48\/\u00b5L, <jats:italic>p<\/jats:italic>\u2009=\u20090.011; 615 vs. 475.12\/\u00b5L, <jats:italic>p<\/jats:italic>\u2009=\u20090.004). In healthy donors, median HPC counts in apheresis products also exceeded CD34\u2009+\u2009cell counts (1,580 vs. 1,430.45\/\u00b5L, <jats:italic>p<\/jats:italic>\u2009=\u20090.005). The HPC cutoffs predicting peripheral blood CD34+\u2009&gt;\u200920\/\u00b5L and a CD34\u2009+\u2009cell yield of \u2265 2\u2009\u00d7\u200910<jats:sup>6<\/jats:sup> cells\/kg were 21\/\u00b5L overall (sensitivity 91.5%, specificity 90.6%), 27\/\u00b5L for multiple myeloma (87.2%, 81.3%), and 20\/\u00b5L for lymphoma (83.3%, 95.2%).<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Conclusions<\/jats:title>\n<jats:p>This study establishes the first disease-specific HPC cutoff values targeting the clinically critical 2\u2009\u00d7\u200910<jats:sup>6<\/jats:sup> cells\/kg threshold: 27 cells\/\u03bcL for multiple myeloma and 20 cells\/\u03bcL for lymphoma. This precision medicine approach advances beyond universal cutoffs, enabling optimized collection timing and potentially reducing healthcare costs.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0333196","type":"journal-article","created":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T17:32:56Z","timestamp":1759944776000},"page":"e0333196","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Establishing disease-specific thresholds for automated hematopoietic progenitor cell counting in hematological malignancies"],"prefix":"10.1371","volume":"20","author":[{"given":"Orathai","family":"Taka","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anchalee","family":"Thedsawad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1262-2005","authenticated-orcid":true,"given":"Weerapat","family":"Owattanapanich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2025,10,8]]},"reference":[{"issue":"8","key":"pone.0333196.ref001","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1038\/bmt.2015.6","article-title":"Indications for allo- and auto-SCT for haematological diseases, solid tumours and immune disorders: current practice in Europe, 2015","volume":"50","author":"A Sureda","year":"2015","journal-title":"Bone Marrow Transplant"},{"issue":"16","key":"pone.0333196.ref002","doi-asserted-by":"crossref","first-page":"1617","DOI":"10.1001\/jama.2010.491","article-title":"Hematopoietic stem cell transplantation: a global perspective","volume":"303","author":"A Gratwohl","year":"2010","journal-title":"JAMA"},{"issue":"9","key":"pone.0333196.ref003","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.1038\/s41409-018-0153-1","article-title":"Is the use of unrelated donor transplantation leveling off in Europe? The 2016 European Society for Blood and Marrow Transplant activity survey report","volume":"53","author":"JR Passweg","year":"2018","journal-title":"Bone Marrow Transplant"},{"key":"pone.0333196.ref004","doi-asserted-by":"crossref","DOI":"10.1038\/sj.bmt.1702343","article-title":"Faster engraftment of neutrophils and platelets with peripheral blood stem cells from unrelated donors: a comparison with marrow transplantation","volume":"25","author":"O Ringd\u00e9n","year":"2000","journal-title":"Bone Marrow Transplant"},{"issue":"1","key":"pone.0333196.ref005","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1080\/14653240310000083","article-title":"Validation of the single-platform ISHAGE method for CD34(+) hematopoietic stem and progenitor cell enumeration in an international multicenter study","volume":"5","author":"JW Gratama","year":"2003","journal-title":"Cytotherapy"},{"issue":"6","key":"pone.0333196.ref006","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1111\/ijlh.12701","article-title":"Evaluation of the Sysmex XN-550, a Novel Compact Haematology analyser from the XN-L\u00ae series, compared to the XN-20 system","volume":"39","author":"H Tailor","year":"2017","journal-title":"Int J Lab Hematol"},{"issue":"1","key":"pone.0333196.ref007","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1007\/s10238-024-01467-0","article-title":"Sysmex XN-HPC: study of reference intervals and clinical decision limits in healthy allogeneic donors mobilised with G-CSF","volume":"24","author":"L Huang","year":"2024","journal-title":"Clin Exp Med"},{"issue":"1","key":"pone.0333196.ref008","first-page":"67","article-title":"Assessment of haematopoietic progenitor cell counting with the Sysmex\u00ae XN-1000 to guide timing of apheresis of peripheral blood stem cells","volume":"18","author":"F 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Kumar","year":"2017","journal-title":"Blood"},{"issue":"1","key":"pone.0333196.ref017","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1111\/ijlh.13341","article-title":"A study to compare Hematopoietic Progenitor Cell count determined on a next-generation automated cell counter with flow cytometric CD34 count in peripheral blood and the harvested peripheral blood stem cell graft from autologous and allogenic donors","volume":"43","author":"S Mishra","year":"2021","journal-title":"Int J Lab Hematol"},{"issue":"8","key":"pone.0333196.ref018","doi-asserted-by":"crossref","first-page":"2001","DOI":"10.1111\/trf.13078","article-title":"Evaluation of new automated hematopoietic progenitor cell analysis in the clinical management of peripheral blood stem cell collections","volume":"55","author":"EI 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