{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T00:21:49Z","timestamp":1758846109936,"version":"3.44.0"},"reference-count":37,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T00:00:00Z","timestamp":1758758400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004493","name":"International Atomic Energy Agency","doi-asserted-by":"publisher","award":["IAEA\/CRP\/D44004\/23918"],"award-info":[{"award-number":["IAEA\/CRP\/D44004\/23918"]}],"id":[{"id":"10.13039\/501100004493","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004493","name":"International Atomic Energy Agency","doi-asserted-by":"publisher","award":["IAEA\/TC\/RAS\/5082"],"award-info":[{"award-number":["IAEA\/TC\/RAS\/5082"]}],"id":[{"id":"10.13039\/501100004493","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004156","name":"Mahidol University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004156","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>Sterile Insect Technique (SIT), Incompatible Insect Technique (IIT) or a combination of the two has become alternative promising vector control approaches. In order to apply these approaches, the targeted mosquitoes need to be sterilized and released. So far, the irradiation of mosquitoes has been conducted at the pupae or adult stages. In this study, we investigated the possibility of applying X-ray irradiation at the egg stage and also assessed the effect on the development and sterility of both wild-type and <jats:italic>Wolbachia<\/jats:italic> trans-infected <jats:italic>Aedes aegypti<\/jats:italic> mosquito vectors. The eggs of both wild-type and <jats:italic>Wolbachia<\/jats:italic> trans-infected lines were irradiated using X-ray at the doses of 1, 3, 5 and 7 Gy. Development of immature stages was observed. For wild-type <jats:italic>Ae. aegypti<\/jats:italic>, X-ray irradiation at the doses from 3 Gy decreased the development of the first-instar larvae and increased the development of the third-instar larvae but there was no effect on pupae. For <jats:italic>Wolbachia<\/jats:italic> trans-infected ones, a irradiation dose as low as 1 Gy could increase the development of the forth-instar larvae while an irradiation dose of 7 Gy induced significantly high mortality to the pupae (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). To assess sterility, males and females that emerged from irradiated eggs were mated with the non-irradiated ones. Our results showed that an irradiation dose of 7 Gy significantly caused more than 90% sterility in both wild-type males and females (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). However, this irradiation dose could be reduced to 5 Gy to sterilize both males and females infected with <jats:italic>Wolbachia<\/jats:italic>. Our findings revealed, for the first time, that applying a low-dose X-ray irradiation at the egg stage could sterilize both wild-type and <jats:italic>Wolbachia<\/jats:italic> trans-infected <jats:italic>Ae. aegypti<\/jats:italic> when they become adults. Egg irradiation could make the implementation of SIT, IIT or combined SIT\/IIT for vector control much more feasible as the sterile eggs are easier to distribute and operate when compared to other developmental stages of mosquitoes.<\/jats:p>","DOI":"10.1371\/journal.pone.0333297","type":"journal-article","created":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T17:41:45Z","timestamp":1758822105000},"page":"e0333297","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effect of egg irradiation on development and sterility of wild-type and Wolbachia trans-infected Aedes aegypti mosquito 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