{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T03:00:21Z","timestamp":1778814021217,"version":"3.51.4"},"reference-count":48,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T00:00:00Z","timestamp":1769644800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82202389"],"award-info":[{"award-number":["82202389"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Postdoctoral Research Fund of Jinling Hospital","award":["97103"],"award-info":[{"award-number":["97103"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      CXCR4\n                      <jats:sup>hi<\/jats:sup>\n                      neutrophil mobilization is a key cause of myocardial damage after acute myocardial infarction (AMI). 6-Phosphofructo-2-kinase\/fructose-2,6-biphosphatase 3 (PFKFB3), a key glycolytic enzyme, plays a crucial role in regulating neutrophil function. However, researchers have not clearly determined whether PFKFB3 is involved in AMI-induced CXCR4\n                      <jats:sup>hi<\/jats:sup>\n                      neutrophil mobilization.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      First, the circulating CXCR4\n                      <jats:sup>hi<\/jats:sup>\n                      neutrophil percentage and neutrophil\n                      <jats:italic>Pfkfb3<\/jats:italic>\n                      mRNA expression were measured in AMI patients and left anterior descending coronary artery (LADCA)-ligated mice. Next, we explored the relationship between PFKFB3 and CXCR4 expression in lipopolysaccharide (LPS)-stimulated cell models. Neu-PFKFB3\n                      <jats:sup>\u2013\/\u2013<\/jats:sup>\n                      mice were used to investigate the effect of conditional knockout of the\n                      <jats:italic>Pfkfb3<\/jats:italic>\n                      gene in neutrophils on AMI-induced myocardial inflammatory injury.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In AMI patients, the expression level of\n                      <jats:italic>Pfkfb<\/jats:italic>\n                      3 gene was markedly regulated in AMI-induced neutrophils and was positively related to the content of plasma inflammatory factors in AMI patients. Further study revealed that PFKFB3 promotes CXCR4\n                      <jats:sup>hi<\/jats:sup>\n                      neutrophil mobilization by reprogramming glycolytic metabolism and subsequently exacerbates inflammatory injury in the myocardial tissues of AMI model mice. However, specific knockout of\n                      <jats:italic>Pfkfb3<\/jats:italic>\n                      gene in neutrophils protects mice from AMI-induced myocardial inflammatory injury by inhibiting the mobilization of CXCR4\n                      <jats:sup>hi<\/jats:sup>\n                      neutrophils.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      PFKFB3 exacerbates AMI-induced myocardial inflammatory injury by accelerating CXCR4\n                      <jats:sup>hi<\/jats:sup>\n                      neutrophil mobilization. The mechanism involves PFKFB3-mediated reprogramming of glycolytic metabolism.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pone.0333657","type":"journal-article","created":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T18:22:56Z","timestamp":1769710976000},"page":"e0333657","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["PFKFB3 exacerbates myocardial injury by accelerating CXCR4hi neutrophil mobilization after acute myocardial infarction"],"prefix":"10.1371","volume":"21","author":[{"given":"Yingjia","family":"Xu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qin","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wutao","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danrui","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dadong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0150-5652","authenticated-orcid":true,"given":"Zongying","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2026,1,29]]},"reference":[{"issue":"10191","key":"pone.0333657.ref001","doi-asserted-by":"crossref","first-page":"2580","DOI":"10.1016\/S0140-6736(19)31419-9","article-title":"Acute myocardial infarction","volume":"393","author":"R Barnett","year":"2019","journal-title":"Lancet"},{"issue":"8","key":"pone.0333657.ref002","article-title":"In-Hospital Mortality in Patients With Acute Myocardial Infarction: A Literature Overview","volume":"16","author":"KA Alnemer","year":"2024","journal-title":"Cureus"},{"key":"pone.0333657.ref003","unstructured":"Hensey M, Cronin M, Keelan E, O\u2019Neill J, Galvin J. 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