{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T11:44:52Z","timestamp":1787917492861,"version":"build-2784847793"},"reference-count":54,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T00:00:00Z","timestamp":1759190400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"CSIRO Postdoctoral Research Fund"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p><jats:italic><jats:italic>Aedes aegypti<\/jats:italic><\/jats:italic> and <jats:italic><jats:italic>Aedes albopictus<\/jats:italic><\/jats:italic> are the most competent vectors of multiple human arboviral diseases, posing a global public health concern, particularly in Bangladesh. While there are numerous genomic resources available worldwide, the genomic resources from Southeast Asian countries are scarce. Current study reported the first characterization of the complete mitochondrial (mt) genomes of <jats:italic><jats:italic>Ae. aegypti<\/jats:italic><\/jats:italic> and <jats:italic><jats:italic>Ae. albopictus<\/jats:italic><\/jats:italic> from Bangladesh. The circular mitogenomes are 16,662\u2009bp and 16,585\u2009bp in size, with AT contents of 79.02% and 78.81%, respectively. Both mitogenomes contain all 37 functional subunits, including 13 protein-coding genes (PCGs), two ribosomal RNA genes, 22 transfer RNA genes (tRNAs), and a control region. We also reported the unique codon usage and Relative synonymous codon usage patterns, which are congruent with other members of the Culicine family. Our intraspecific analysis of 15 mitochondrial genes revealed higher genetic distance, but lower SNP density in <jats:italic><jats:italic>Ae. aegypti<\/jats:italic><\/jats:italic>, compared to <jats:italic><jats:italic>Ae. albopictus<\/jats:italic><\/jats:italic>, indicating <jats:italic><jats:italic>Ae. aegypti<\/jats:italic><\/jats:italic> might have a longer evolutionary history, while <jats:italic><jats:italic>Ae. albopictus<\/jats:italic><\/jats:italic> might have experienced recent population expansion with steady divergence. Similarity metrics and phylogeny of 13 PCGs from 37 taxa indicated both species of <jats:italic><jats:italic>Aedes<\/jats:italic><\/jats:italic> formed specific clusters, where Bangladeshi <jats:italic><jats:italic>Aedes<\/jats:italic><\/jats:italic> spp<jats:italic>.<\/jats:italic> showed close relationships with North American, Oceania and European populations. Our comprehensive tree incorporating <jats:italic><jats:italic>Aedes, Ochleroratus and Culex<\/jats:italic><\/jats:italic> spp<jats:italic>.<\/jats:italic> revealed distinct clades, aligning with previous studies. Current study provides a foundation for future research on <jats:italic><jats:italic>Aedes<\/jats:italic><\/jats:italic> spp<jats:italic>.<\/jats:italic> of Bangladesh, leveraging it to other Southeast Asian countries, aiding vector identification, control, and disease mitigation.<\/jats:p>","DOI":"10.1371\/journal.pone.0333693","type":"journal-article","created":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T17:37:32Z","timestamp":1759253852000},"page":"e0333693","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["Complete mitogenomes of two major dengue vectors Aedes aegypti and Aedes albopictus from Bangladesh: Insights from comparative genomics with global mitogenome diversity and phylogenetics"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-3838-0085","authenticated-orcid":true,"given":"Md. 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region","volume":"14","author":"AA Sousa","year":"2023","journal-title":"Insects"},{"issue":"12","key":"pone.0333693.ref038","doi-asserted-by":"crossref","first-page":"1983","DOI":"10.3390\/genes12121983","article-title":"First Description of the Mitogenome and Phylogeny of Culicinae Species from the Amazon Region","volume":"12","author":"BLS do Nascimento","year":"2021","journal-title":"Genes (Basel)"},{"key":"pone.0333693.ref039","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1146\/annurev-ento-011613-162007","article-title":"Insect mitochondrial genomics: implications for evolution and phylogeny","volume":"59","author":"SL Cameron","year":"2014","journal-title":"Annu Rev Entomol"},{"issue":"1","key":"pone.0333693.ref040","doi-asserted-by":"crossref","first-page":"3412","DOI":"10.1038\/s41598-019-39895-8","article-title":"The complete mitochondrial genomes of two sibling species of camellia weevils (Coleoptera: Curculionidae) and patterns of Curculionini speciation","volume":"9","author":"S-K Zhang","year":"2019","journal-title":"Sci Rep"},{"issue":"1","key":"pone.0333693.ref041","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1080\/23802359.2024.2447750","article-title":"Complete mitochondrial genomes of Culicoides brevitarsis and Culicoides imicola biting midge vectors of Bluetongue Virus","volume":"10","author":"KA Ahmed","year":"2025","journal-title":"Mitochondrial DNA B Resour"},{"issue":"7732","key":"pone.0333693.ref042","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1038\/s41586-018-0692-z","article-title":"Improved reference genome of Aedes aegypti informs arbovirus vector control","volume":"563","author":"BJ Matthews","year":"2018","journal-title":"Nature"},{"issue":"3","key":"pone.0333693.ref043","first-page":"2024","article-title":"The complete mitochondrial DNA genomes for two lineages of Aedes notoscriptus (Diptera: Culicidae)","volume":"27","author":"CM 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