{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T17:14:05Z","timestamp":1789578845341,"version":"build-2803163510"},"reference-count":131,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T00:00:00Z","timestamp":1765497600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100020628","name":"ICT Division, Bangladesh","doi-asserted-by":"crossref","award":["24IF27334"],"award-info":[{"award-number":["24IF27334"]}],"id":[{"id":"10.13039\/100020628","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    <jats:italic>Lysinibacillus boronitolerans<\/jats:italic>\n                    MSR1, a bacterium isolated from yogurt, was characterized through a detailed genomic and phylogenetic analysis. The strain demonstrated the ability to grow in MRS broth at pH 5\u20138 and exhibited positive activity in citrate utilization, catalase, oxidase, Methyl Red\u2013Voges Proskauer (MR-VP), and Triple Sugar Iron (TSI) tests while being negative for amylase degradation and sulfide production. Whole-genome sequencing and Average Nucleotide Identity (ANI) analysis revealed a close genetic relationship between MSR1 and previously reported\n                    <jats:italic>L. boronitolerans<\/jats:italic>\n                    strains, with ANI values ranging from 95.25% to 98.30%, particularly for\n                    <jats:italic>L. boronitolerans strain<\/jats:italic>\n                    NBRC103108. Phylogenetic analyses based on whole-genome and 16S rRNA sequences confirmed the taxonomic placement of MSR1 within the\n                    <jats:italic>L. boronitolerans<\/jats:italic>\n                    species. A circular genome comparison highlighted the presence of unique genomic regions in MSR1, notably around the 3500 kbp mark, indicating the acquisition of novel genes that may contribute to its distinct phenotypic traits. Antibiotic susceptibility testing revealed a high level of resistance in MSR1 to glycopeptides and aminoglycosides, while the strain remained susceptible to imipenem, with in silico analysis identifying key antimicrobial resistance (AMR) genes, including\n                    <jats:italic>qacJ<\/jats:italic>\n                    ,\n                    <jats:italic>vanW<\/jats:italic>\n                    ,\n                    <jats:italic>vanT<\/jats:italic>\n                    , and\n                    <jats:italic>FosBx1<\/jats:italic>\n                    , which confer resistance to disinfectants, vancomycin, and fosfomycin through efflux pumps and target modification mechanisms. Five distinct biosynthetic gene cluster (BGC) regions were identified in the MSR1 genome, encoding genes for lanthipeptide-class-iii, RiPP-like, T3PKS, beta-lactone, terpene, and NRPS-like clusters. Pan-genome analysis suggested that\n                    <jats:italic>L. boronitolerans<\/jats:italic>\n                    possesses an open pan-genome, with a substantial proportion of accessory and unique genes. Functional annotation of core, accessory, and unique genes revealed that core genes are predominantly associated with metabolic processes, while accessory and unique genes are involved in information processing, storage, and defence mechanisms. These findings enhance our understanding of the genomic diversity, evolutionary dynamics, and potential adaptive strategies of\n                    <jats:italic>L. boronitolerans<\/jats:italic>\n                    MSR1, providing new insights into its ecological and functional roles.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0333844","type":"journal-article","created":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T18:23:07Z","timestamp":1765563787000},"page":"e0333844","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Whole-genome analysis of Lysinibacillus boronitolerans MSR1: A dairy-isolated multidrug-resistant and non-pathogenic strain"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-2028-4885","authenticated-orcid":true,"given":"Md. Shahedur","family":"Rahman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Tofidul","family":"Islam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-1821-630X","authenticated-orcid":true,"given":"Mahadi","family":"Hasan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md.","family":"Tariquzzaman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nure Sharaf Nower","family":"Samia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Mahfuzur","family":"Rahman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mustafizur","family":"Rahman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,12,12]]},"reference":[{"key":"pone.0333844.ref001","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/B978-0-12-805134-4.00028-6","article-title":"Role of yogurt in the nutrition and health of children and adolescents","volume-title":"Yogurt Health Disease Prevent","author":"MA Fernandez","year":"2017"},{"issue":"20","key":"pone.0333844.ref002","doi-asserted-by":"crossref","DOI":"10.3390\/nu15204366","article-title":"Nutritional value of yogurt as a protein source: digestibility\/absorbability and effects on skeletal muscle","volume":"15","author":"K Sumi","year":"2023","journal-title":"Nutrients"},{"issue":"8","key":"pone.0333844.ref003","doi-asserted-by":"crossref","DOI":"10.1136\/bmjopen-2017-021387","article-title":"Evaluation of the nutrient content of yogurts: a comprehensive survey of yogurt products in the major UK supermarkets","volume":"8","author":"JB Moore","year":"2018","journal-title":"BMJ Open"},{"issue":"1","key":"pone.0333844.ref004","doi-asserted-by":"crossref","first-page":"7089","DOI":"10.1038\/s41598-025-90999-w","article-title":"Comprehensive genomics, probiotic, and antibiofilm potential analysis of Streptococcus thermophilus strains isolated from homemade and commercial dahi","volume":"15","author":"A Sudheer","year":"2025","journal-title":"Sci Rep"},{"issue":"1","key":"pone.0333844.ref005","doi-asserted-by":"crossref","first-page":"176","DOI":"10.3390\/foods3010176","article-title":"Conventional and innovative processing of milk for yogurt manufacture; development of texture and flavor: a 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Contaminants and Their Toxins in Fermented Dairy Products: a Comprehensive Review","volume":"15","author":"AS El-Sayed","year":"2022","journal-title":"Food Anal Methods"},{"issue":"1","key":"pone.0333844.ref009","doi-asserted-by":"crossref","DOI":"10.1111\/jfs.12741","article-title":"Microbial safety and probiotic potential of packaged yogurt products in Pakistan","volume":"40","author":"G Aziz","year":"2019","journal-title":"Journal of Food Safety"},{"issue":"17","key":"pone.0333844.ref010","doi-asserted-by":"crossref","DOI":"10.3390\/foods11172572","article-title":"Bacillus cereus in dairy products and production plants","volume":"11","author":"E Tirloni","year":"2022","journal-title":"Foods"},{"issue":"1","key":"pone.0333844.ref011","doi-asserted-by":"crossref","first-page":"82","DOI":"10.4103\/0974-777X.93768","article-title":"Recurrent sepsis due to bacillus licheniformis","volume":"4","author":"IA Haydushka","year":"2012","journal-title":"J Glob Infect Dis"},{"issue":"21","key":"pone.0333844.ref012","doi-asserted-by":"crossref","first-page":"5988","DOI":"10.1128\/JB.01485-12","article-title":"Genome sequence of Lysinibacillus boronitolerans F1182, isolated from a traditional Korean fermented soybean product","volume":"194","author":"Y-D Nam","year":"2012","journal-title":"J Bacteriol"},{"key":"pone.0333844.ref013","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1099\/ijs.0.63867-0","article-title":"Proposal of Lysinibacillus boronitolerans gen. nov. sp. nov., and transfer of Bacillus fusiformis to Lysinibacillus fusiformis comb. nov. and Bacillus sphaericus to Lysinibacillus sphaericus comb. nov","volume":"57","author":"I Ahmed","year":"2007","journal-title":"Int J Syst Evol Microbiol"},{"issue":"12","key":"pone.0333844.ref014","article-title":"Lysinibacilli: A biological factories intended for bio-insecticidal, bio-control, and bioremediation activities","volume":"8","author":"QMS Jamal","year":"2022","journal-title":"J 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Lysinibacillus sphaericus, Lysinibacillus fusiformis and Paenibacillus amylolyticus Bacteremia","volume":"35","author":"E Wenzler","year":"2015","journal-title":"Int J Infect Dis"},{"issue":"7","key":"pone.0333844.ref022","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1007\/s10482-023-01828-x","article-title":"Lysinibacillus spp.: an IAA-producing endospore forming-bacteria that promotes plant growth","volume":"116","author":"M Pantoja-Guerra","year":"2023","journal-title":"Antonie Van Leeuwenhoek"},{"issue":"11","key":"pone.0333844.ref023","doi-asserted-by":"crossref","DOI":"10.1099\/ijsem.0.006167","article-title":"Lysinibacillus irui sp. nov., isolated from Iru, fermented African locust beans","volume":"73","author":"SO Akintayo","year":"2023","journal-title":"Int J Syst Evol Microbiol"},{"issue":"5","key":"pone.0333844.ref024","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0299588","article-title":"Comprehensive analysis of genomic variation, pan-genome and biosynthetic potential of Corynebacterium glutamicum strains","volume":"19","author":"MS Rahman","year":"2024","journal-title":"PLoS One"},{"issue":"8","key":"pone.0333844.ref025","doi-asserted-by":"crossref","first-page":"3277","DOI":"10.1007\/s00253-019-09708-z","article-title":"Detecting and prioritizing biosynthetic gene clusters for bioactive compounds in bacteria and fungi","volume":"103","author":"PN Tran","year":"2019","journal-title":"Appl Microbiol Biotechnol"},{"key":"pone.0333844.ref026","doi-asserted-by":"crossref","first-page":"105046","DOI":"10.1016\/j.compbiomed.2021.105046","article-title":"In silico genome mining of potential novel biosynthetic gene clusters for drug discovery from Burkholderia bacteria","volume":"140","author":"K Alam","year":"2022","journal-title":"Comput Biol Med"},{"issue":"5","key":"pone.0333844.ref027","article-title":"Whole genome sequencing and comparative genomic analyses of Lysinibacillus pakistanensis LZH-9, a halotolerant strain with excellent COD removal capability","volume":"8","author":"X Wu","year":"2020","journal-title":"Microorganisms"},{"key":"pone.0333844.ref028","doi-asserted-by":"crossref","first-page":"1521632","DOI":"10.3389\/fmicb.2025.1521632","article-title":"Isolation and characterization of a novel highly efficient bacterium Lysinibacillus boronitolerans QD4 for quantum dot biosynthesis","volume":"16","author":"X Gu","year":"2025","journal-title":"Front Microbiol"},{"issue":"2","key":"pone.0333844.ref029","doi-asserted-by":"crossref","first-page":"100457","DOI":"10.1016\/j.afres.2024.100457","article-title":"Isolation and characterization of probiotic lactic acid bacteria from local yogurt and development of inulin-based synbiotic yogurt with the isolated bacteria","volume":"4","author":"MdS Rahman","year":"2024","journal-title":"Appl Food Res"},{"issue":"6","key":"pone.0333844.ref030","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1306\/042604740858","article-title":"Effects of SEM Preparation Techniques on the Appearance of Bacteria and Biofilms in the Carter Sandstone","volume":"74","author":"SE Fratesi","year":"2004","journal-title":"J Sedimentary Res"},{"key":"pone.0333844.ref031","article-title":"Collins and Lyne\u2019s microbiological methods","author":"PM Lyne","year":"1995"},{"issue":"2","key":"pone.0333844.ref032","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1080\/01490451.2019.1666938","article-title":"Arsenic remediation in Bangladeshi rice varieties with enhance plant growth by unique arsenic-resistant bacterial isolates","volume":"37","author":"MS Rahman","year":"2019","journal-title":"Geomicrobiol J"},{"issue":"8","key":"pone.0333844.ref033","doi-asserted-by":"crossref","first-page":"5075","DOI":"10.1007\/s00203-021-02469-2","article-title":"Diesel degradation efficiency of Enterobacter sp., Acinetobacter sp., and Cedecea sp. isolated from petroleum waste dumping site: a bioremediation view point","volume":"203","author":"I Jerin","year":"2021","journal-title":"Archiv Microbiol"},{"issue":"5","key":"pone.0333844.ref034","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1002\/jobm.201900570","article-title":"Efficacy of soil-borne Enterobacter sp. for carbofuran degradation: HPLC quantitation of degradation rate","volume":"60","author":"MA-E Ekram","year":"2020","journal-title":"J Basic Microbiol"},{"key":"pone.0333844.ref035","volume-title":"Preparation of Routine Media and Reagents Used in Antimicrobial Susceptibility Testing","author":"DR Lonsway","year":"2023","edition":"5"},{"key":"pone.0333844.ref036","volume-title":"Performance standards for antimicrobial susceptibility testing","author":"LS Institute C a","year":"2020","edition":"30"},{"issue":"8","key":"pone.0333844.ref037","doi-asserted-by":"crossref","first-page":"2461","DOI":"10.1128\/AEM.02272-07","article-title":"Critical evaluation of two primers commonly used for amplification of bacterial 16S rRNA genes","volume":"74","author":"JA Frank","year":"2008","journal-title":"Appl Environ Microbiol"},{"issue":"6","key":"pone.0333844.ref038","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0305650","article-title":"Comparison of DeNovix, NanoDrop and Qubit for DNA quantification and impurity detection of bacterial DNA extracts","volume":"19","author":"N Versmessen","year":"2024","journal-title":"PLoS One"},{"key":"pone.0333844.ref039","article-title":"FastQC: a quality control tool for high throughput sequence data","author":"S Andrews","year":"2010","journal-title":"Babraham Bioinformatics"},{"key":"pone.0333844.ref040","volume-title":"Sickle: A sliding-window, adaptive, quality-based trimming tool for FastQ files","author":"NA Joshi","year":"2011"},{"issue":"1","key":"pone.0333844.ref041","doi-asserted-by":"crossref","DOI":"10.1002\/cpbi.102","article-title":"Using SPAdes de novo assembler","volume":"70","author":"A Prjibelski","year":"2020","journal-title":"Curr Protoc Bioinformatics"},{"issue":"11","key":"pone.0333844.ref042","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0112963","article-title":"Pilon: an integrated tool for comprehensive microbial variant detection and genome assembly improvement","volume":"9","author":"BJ Walker","year":"2014","journal-title":"PLoS One"},{"key":"pone.0333844.ref043","doi-asserted-by":"crossref","first-page":"124","DOI":"10.12688\/wellcomeopenres.14826.1","article-title":"Open-access bacterial population genomics: BIGSdb software, the PubMLST.org website and their applications","volume":"3","author":"KA Jolley","year":"2018","journal-title":"Wellcome Open Res"},{"issue":"1","key":"pone.0333844.ref044","doi-asserted-by":"crossref","first-page":"2182","DOI":"10.1038\/s41467-019-10210-3","article-title":"TYGS is an automated high-throughput platform for state-of-the-art genome-based taxonomy","volume":"10","author":"JP Meier-Kolthoff","year":"2019","journal-title":"Nat Commun"},{"issue":"14","key":"pone.0333844.ref045","doi-asserted-by":"crossref","first-page":"2068","DOI":"10.1093\/bioinformatics\/btu153","article-title":"Prokka: rapid prokaryotic genome annotation","volume":"30","author":"T Seemann","year":"2014","journal-title":"Bioinformatics"},{"key":"pone.0333844.ref046","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkad326","article-title":"Proksee: in-depth characterization and visualization of bacterial genomes","volume":"51","author":"JR Grant","year":"2023","journal-title":"Nucleic Acids Res"},{"key":"pone.0333844.ref047","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1186\/1471-2164-12-402","article-title":"BLAST ring image generator (BRIG): simple prokaryote genome comparisons","volume":"12","author":"N-F Alikhan","year":"2011","journal-title":"BMC Genomics"},{"issue":"2","key":"pone.0333844.ref048","doi-asserted-by":"crossref","first-page":"1100","DOI":"10.1099\/ijsem.0.000760","article-title":"OrthoANI: An improved algorithm and software for calculating average nucleotide identity","volume":"66","author":"I Lee","year":"2016","journal-title":"Int J Syst Evol Microbiol"},{"issue":"11","key":"pone.0333844.ref049","doi-asserted-by":"crossref","first-page":"1733","DOI":"10.1016\/j.molp.2023.09.010","article-title":"TBtools-II: A \u201cone for all, all for one\u201d bioinformatics platform for biological big-data mining","volume":"16","author":"C Chen","year":"2023","journal-title":"Mol Plant"},{"key":"pone.0333844.ref050","unstructured":"TS. Snippy: fast bacterial variant calling from NGS reads. 2015."},{"key":"pone.0333844.ref051","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkad344","article-title":"antiSMASH 7.0: new and improved predictions for detection, regulation, chemical structures and visualisation","volume":"51","author":"K Blin","year":"2023","journal-title":"Nucleic Acids Res"},{"issue":"1","key":"pone.0333844.ref052","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1186\/1471-2164-15-717","article-title":"HGTector: an automated method facilitating genome-wide discovery of putative horizontal gene transfers","volume":"15","author":"Q Zhu","year":"2014","journal-title":"BMC Genomics"},{"issue":"3","key":"pone.0333844.ref053","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1093\/jac\/dkab450","article-title":"Antimicrobial-induced horizontal transfer of antimicrobial resistance genes in bacteria: a mini-review","volume":"77","author":"G Liu","year":"2022","journal-title":"J Antimicrob Chemother"},{"key":"pone.0333844.ref054","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkac920","article-title":"CARD 2023: expanded curation, support for machine learning, and resistome prediction at the Comprehensive Antibiotic Resistance Database","volume":"51","author":"BP Alcock","year":"2023","journal-title":"Nucleic Acids Res"},{"key":"pone.0333844.ref055","doi-asserted-by":"crossref","first-page":"104334","DOI":"10.1016\/j.fm.2023.104334","article-title":"Pangenome analysis reveals the genetic basis for taxonomic classification of the Lactobacillaceae family","volume":"115","author":"A Rajput","year":"2023","journal-title":"Food Microbiol"},{"key":"pone.0333844.ref056","doi-asserted-by":"crossref","first-page":"24373","DOI":"10.1038\/srep24373","article-title":"BPGA- an ultra-fast pan-genome analysis pipeline","volume":"6","author":"NM Chaudhari","year":"2016","journal-title":"Sci Rep"},{"issue":"22","key":"pone.0333844.ref057","doi-asserted-by":"crossref","first-page":"3691","DOI":"10.1093\/bioinformatics\/btv421","article-title":"Roary: rapid large-scale prokaryote pan genome analysis","volume":"31","author":"AJ Page","year":"2015","journal-title":"Bioinformatics"},{"issue":"19","key":"pone.0333844.ref058","doi-asserted-by":"crossref","first-page":"2460","DOI":"10.1093\/bioinformatics\/btq461","article-title":"Search and clustering orders of magnitude faster than BLAST","volume":"26","author":"RC Edgar","year":"2010","journal-title":"Bioinformatics"},{"issue":"7","key":"pone.0333844.ref059","doi-asserted-by":"crossref","first-page":"3895","DOI":"10.1128\/AAC.02412-14","article-title":"In silico detection and typing of plasmids using PlasmidFinder and plasmid multilocus sequence typing","volume":"58","author":"A Carattoli","year":"2014","journal-title":"Antimicrob Agents Chemother"},{"key":"pone.0333844.ref060","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1186\/1471-2105-10-421","article-title":"BLAST+: architecture and applications","volume":"10","author":"C Camacho","year":"2009","journal-title":"BMC Bioinformatics"},{"issue":"10","key":"pone.0333844.ref061","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.01269-20","article-title":"In silico genotyping of escherichia coli isolates for extraintestinal virulence genes by use of whole-genome sequencing data","volume":"58","author":"AM Malberg Tetzschner","year":"2020","journal-title":"J Clin Microbiol"},{"issue":"10","key":"pone.0333844.ref062","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0077302","article-title":"PathogenFinder--distinguishing friend from foe using bacterial whole genome sequence data","volume":"8","author":"S Cosentino","year":"2013","journal-title":"PLoS One"},{"issue":"5","key":"pone.0333844.ref063","doi-asserted-by":"crossref","first-page":"1501","DOI":"10.1128\/JCM.03617-13","article-title":"Real-time whole-genome sequencing for routine typing, surveillance, and outbreak detection of verotoxigenic Escherichia coli","volume":"52","author":"KG Joensen","year":"2014","journal-title":"J Clin Microbiol"},{"issue":"3","key":"pone.0333844.ref064","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.mimet.2013.09.020","article-title":"Improvements on colony morphology identification towards bacterial profiling","volume":"95","author":"AM Sousa","year":"2013","journal-title":"J Microbiol Methods"},{"key":"pone.0333844.ref065","doi-asserted-by":"crossref","first-page":"1427","DOI":"10.1099\/ijs.0.65455-0","article-title":"Lysinibacillus parviboronicapiens sp. nov., a low-boron-containing bacterium isolated from soil","volume":"59","author":"H Miwa","year":"2009","journal-title":"Int J Syst Evol 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Khachatryan","year":"2020","journal-title":"Forensic Sci Int Genet"},{"issue":"1","key":"pone.0333844.ref072","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1007\/s13205-015-0332-3","article-title":"Characterization of antibiotic resistant and enzyme producing bacterial strains isolated from the Arabian Sea","volume":"6","author":"PN Tallur","year":"2016","journal-title":"3 Biotech"},{"issue":"8","key":"pone.0333844.ref073","doi-asserted-by":"crossref","first-page":"1558","DOI":"10.1007\/s00284-020-01976-7","article-title":"Screening and Optimization of Newly Isolated Thermotolerant Lysinibacillus fusiformis Strain SK for Protease and Antifungal Activity","volume":"77","author":"S Khadka","year":"2020","journal-title":"Curr Microbiol"},{"issue":"8","key":"pone.0333844.ref074","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0183607","article-title":"Molecular characterization of vancomycin-resistant Staphylococcus aureus strains isolated from clinical samples: a 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inhibitors","volume":"169","author":"A Gaurav","year":"2023","journal-title":"Microbiology (Reading)"},{"issue":"1","key":"pone.0333844.ref081","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.1038\/s42003-021-02629-6","article-title":"The Acinetobacter baumannii disinfectant resistance protein, AmvA, is a spermidine and spermine efflux pump","volume":"4","author":"FL Short","year":"2021","journal-title":"Commun Biol"},{"issue":"7","key":"pone.0333844.ref082","article-title":"Efflux pump inhibitors in controlling antibiotic resistance: outlook under a heavy metal contamination context","volume":"28","author":"THT Nguyen","year":"2023","journal-title":"Molecules"},{"issue":"4","key":"pone.0333844.ref083","doi-asserted-by":"crossref","DOI":"10.3390\/microorganisms11041065","article-title":"Prevalence and antibiotic resistance of Bacillus sp. isolated from raw milk","volume":"11","author":"P Adamski","year":"2023","journal-title":"Microorganisms"},{"key":"pone.0333844.ref084","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.jgar.2021.12.016","article-title":"Glycopeptide resistance in Enterococcus spp. and coagulase-negative staphylococci from hospitalised patients in Germany: occurrence, characteristics and dalbavancin susceptibility","volume":"28","author":"M Kresken","year":"2022","journal-title":"J Glob Antimicrob Resist"},{"issue":"1","key":"pone.0333844.ref085","doi-asserted-by":"crossref","first-page":"24","DOI":"10.3390\/microorganisms11010024","article-title":"Vancomycin Resistance in Enterococcus and Staphylococcus aureus","volume":"11","author":"G Li","year":"2022","journal-title":"Microorganisms"},{"issue":"4","key":"pone.0333844.ref086","doi-asserted-by":"crossref","DOI":"10.3390\/cells8040309","article-title":"Multiple lines of evidences reveal mechanisms underpinning mercury resistance and volatilization by Stenotrophomonas sp. MA5 isolated from the Savannah River Site (SRS), USA","volume":"8","author":"M Agarwal","year":"2019","journal-title":"Cells"},{"key":"pone.0333844.ref087","doi-asserted-by":"crossref","first-page":"100882","DOI":"10.1016\/j.cofs.2022.100882","article-title":"Role of horizontal gene transfer in the dissemination of antimicrobial resistance in food animal production","volume":"47","author":"PG Vinayamohan","year":"2022","journal-title":"Current Opinion in Food Sci"},{"issue":"43","key":"pone.0333844.ref088","doi-asserted-by":"crossref","first-page":"26868","DOI":"10.1073\/pnas.2005331117","article-title":"Horizontal gene transfer potentiates adaptation by reducing selective constraints on the spread of genetic variation","volume":"117","author":"LC Woods","year":"2020","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"2","key":"pone.0333844.ref089","doi-asserted-by":"crossref","first-page":"328","DOI":"10.3390\/antibiotics12020328","article-title":"Horizontal gene transfer of antibiotic resistance genes in biofilms","volume":"12","author":"C Michaelis","year":"2023","journal-title":"Antibiotics (Basel)"},{"issue":"7","key":"pone.0333844.ref090","doi-asserted-by":"crossref","first-page":"2643","DOI":"10.3390\/ijerph10072643","article-title":"Antimicrobial resistance in the food chain: a review","volume":"10","author":"C Verraes","year":"2013","journal-title":"Int J Environ Res Public Health"},{"key":"pone.0333844.ref091","doi-asserted-by":"crossref","first-page":"822689","DOI":"10.3389\/fmicb.2022.822689","article-title":"A review of current bacterial resistance to antibiotics in food animals","volume":"13","author":"C Xu","year":"2022","journal-title":"Front Microbiol"},{"issue":"1","key":"pone.0333844.ref092","doi-asserted-by":"crossref","first-page":"100027","DOI":"10.1016\/j.agrcom.2024.100027","article-title":"Antibiotic resistant bacteria in food systems: Current status, resistance mechanisms, and mitigation strategies","volume":"2","author":"SA Okaiyeto","year":"2024","journal-title":"Agriculture Communications"},{"issue":"1","key":"pone.0333844.ref093","doi-asserted-by":"crossref","DOI":"10.3390\/mps3010021","article-title":"A rapid and high throughput MIC determination method to screen uranium resistant microorganisms","volume":"3","author":"M Agarwal","year":"2020","journal-title":"Methods Protoc"},{"issue":"7","key":"pone.0333844.ref094","doi-asserted-by":"crossref","DOI":"10.3390\/microorganisms10071274","article-title":"Microbial genetics and evolution","volume":"10","author":"S Del Duca","year":"2022","journal-title":"Microorganisms"},{"issue":"1","key":"pone.0333844.ref095","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1574-6976.2000.tb00529.x","article-title":"Genetic variation: molecular mechanisms and impact on microbial evolution","volume":"24","author":"W Arber","year":"2000","journal-title":"FEMS Microbiol Rev"},{"issue":"1","key":"pone.0333844.ref096","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1007\/s00253-024-13210-6","article-title":"Comparative genomics reveals insights into the potential of Lysinibacillus irui as a plant growth promoter","volume":"108","author":"S Hil\u00e1rio","year":"2024","journal-title":"Appl Microbiol Biotechnol"},{"key":"pone.0333844.ref097","doi-asserted-by":"crossref","first-page":"1271418","DOI":"10.3389\/fmicb.2023.1271418","article-title":"Species-specificity of the secondary biosynthetic potential in Bacillus","volume":"14","author":"Q-J Yin","year":"2023","journal-title":"Front Microbiol"},{"issue":"5","key":"pone.0333844.ref098","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1111\/j.1574-6976.2011.00292.x","article-title":"Origins of bacterial diversity through horizontal genetic transfer and adaptation to new ecological niches","volume":"35","author":"J Wiedenbeck","year":"2011","journal-title":"FEMS Microbiol 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Tang","year":"2023","journal-title":"Nucleic Acids Res"},{"issue":"3","key":"pone.0333844.ref105","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1093\/bib\/bbx162","article-title":"Assessing the heterogeneity of in silico plasmid predictions based on whole-genome-sequenced clinical isolates","volume":"20","author":"CC Laczny","year":"2019","journal-title":"Brief Bioinform"},{"key":"pone.0333844.ref106","doi-asserted-by":"crossref","first-page":"2889","DOI":"10.3762\/bjoc.15.283","article-title":"Bacterial terpene biosynthesis: challenges and opportunities for pathway engineering","volume":"15","author":"EJN Helfrich","year":"2019","journal-title":"Beilstein J Org Chem"},{"issue":"12","key":"pone.0333844.ref107","doi-asserted-by":"crossref","DOI":"10.3390\/foods10123153","article-title":"Profile of volatile compounds in dessert yogurts prepared from cow and goat milk, using different starter cultures and probiotics","volume":"10","author":"G Papaioannou","year":"2021","journal-title":"Foods"},{"key":"pone.0333844.ref108","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.mib.2018.02.010","article-title":"RiPP antibiotics: biosynthesis and engineering potential","volume":"45","author":"GA Hudson","year":"2018","journal-title":"Curr Opin Microbiol"},{"issue":"7","key":"pone.0333844.ref109","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1039\/D3NP00057E","article-title":"Opportunities and challenges of RiPP-based therapeutics","volume":"41","author":"IP-M Pfeiffer","year":"2024","journal-title":"Nat Prod Rep"},{"issue":"4","key":"pone.0333844.ref110","article-title":"Advancements in the application of ribosomally synthesized and post-translationally modified peptides (RiPPs)","volume":"14","author":"SW Han","year":"2024","journal-title":"Biomolecules"},{"issue":"8","key":"pone.0333844.ref111","doi-asserted-by":"crossref","first-page":"3921","DOI":"10.1007\/s00018-021-03759-0","article-title":"Antiviral activities and applications of ribosomally synthesized and post-translationally modified peptides (RiPPs)","volume":"78","author":"Y Fu","year":"2021","journal-title":"Cell Mol Life Sci"},{"issue":"14","key":"pone.0333844.ref112","article-title":"Therapeutic application of lantibiotics and other lanthipeptides: old and new findings","volume":"87","author":"ADP van Staden","year":"2021","journal-title":"Appl Environ Microbiol"},{"issue":"12","key":"pone.0333844.ref113","doi-asserted-by":"crossref","first-page":"10605","DOI":"10.1007\/s11033-023-08880-w","article-title":"The untapped potential of actinobacterial lanthipeptides as therapeutic agents","volume":"50","author":"D Ram\u00edrez-Rend\u00f3n","year":"2023","journal-title":"Mol Biol 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Bruna","year":"2024","journal-title":"Appl Microbiol Biotechnol"},{"issue":"1","key":"pone.0333844.ref117","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1038\/s41467-022-28668-z","article-title":"Biosynthetic gene cluster profiling predicts the positive association between antagonism and phylogeny in Bacillus","volume":"13","author":"L Xia","year":"2022","journal-title":"Nat Commun"},{"issue":"2","key":"pone.0333844.ref118","doi-asserted-by":"crossref","first-page":"1951","DOI":"10.1093\/bib\/bbaa013","article-title":"Toward a high-quality pan-genome landscape of Bacillus subtilis by removal of confounding strains","volume":"22","author":"H Wu","year":"2021","journal-title":"Brief Bioinform"},{"issue":"5","key":"pone.0333844.ref119","doi-asserted-by":"crossref","DOI":"10.3390\/cells14050369","article-title":"Isolation and functional characterization of endophytic bacteria from muscadine grape berries: a microbial treasure trove","volume":"14","author":"M Agarwal","year":"2025","journal-title":"Cells"},{"issue":"5","key":"pone.0333844.ref120","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1016\/j.mib.2008.09.006","article-title":"Comparative genomics: the bacterial pan-genome","volume":"11","author":"H Tettelin","year":"2008","journal-title":"Curr Opin Microbiol"},{"key":"pone.0333844.ref121","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-030-38281-0","article-title":"In The Pangenome: Diversity, Dynamics and Evolution of Genomes","author":"H Tettelin","year":"2020"},{"issue":"2","key":"pone.0333844.ref122","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.tig.2019.11.006","article-title":"Pangenomics Comes of Age: From Bacteria to Plant and Animal Applications","volume":"36","author":"AA Golicz","year":"2020","journal-title":"Trends Genet"},{"issue":"6","key":"pone.0333844.ref123","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1016\/j.gde.2005.09.006","article-title":"The microbial pan-genome","volume":"15","author":"D Medini","year":"2005","journal-title":"Curr Opin Genet Dev"},{"key":"pone.0333844.ref124","doi-asserted-by":"crossref","first-page":"1076797","DOI":"10.3389\/fmicb.2022.1076797","article-title":"Pan-genome association study of Mycobacterium tuberculosis lineage-4 revealed specific genes related to the high and low prevalence of the disease in patients from the North-Eastern area of Medell\u00edn, Colombia","volume":"13","author":"U Hurtado-P\u00e1ez","year":"2023","journal-title":"Front Microbiol"},{"issue":"5","key":"pone.0333844.ref125","doi-asserted-by":"crossref","DOI":"10.1128\/mSystems.00374-19","article-title":"Distinct drivers of core and accessory components of soil microbial community functional diversity under environmental changes","volume":"4","author":"X Zhang","year":"2019","journal-title":"mSystems"},{"issue":"1","key":"pone.0333844.ref126","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/s12864-021-08223-8","article-title":"Comparative pangenomics: analysis of 12 microbial pathogen pangenomes reveals conserved global structures of genetic and functional diversity","volume":"23","author":"JC Hyun","year":"2022","journal-title":"BMC Genomics"},{"key":"pone.0333844.ref127","first-page":"35","article-title":"Concept of microbial preservation: past, present and future","volume-title":"Soil Biology","author":"R Sharma","year":"2018"},{"key":"pone.0333844.ref128","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.nmni.2015.06.005","article-title":"The bacterial pangenome as a new tool for analysing pathogenic bacteria","volume":"7","author":"L Rouli","year":"2015","journal-title":"New Microbes New 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