{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T22:56:36Z","timestamp":1768258596158,"version":"3.49.0"},"reference-count":61,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:00:00Z","timestamp":1760054400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100019004","name":"Deanship of Research, Jordan University of Science and Technology","doi-asserted-by":"publisher","award":["15\/2020"],"award-info":[{"award-number":["15\/2020"]}],"id":[{"id":"10.13039\/501100019004","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>Multiple sclerosis (MS) is believed to result from a complex interplay of behavioral, genetic, and environmental risk factors. Furthermore, some studies indicated that vitamin D deficiency is linked to the emergence of different diseases, including MS. This study aims to determine the genetic associations between vitamin D metabolism gene polymorphisms and MS susceptibility in the Jordanian community. A total of 388 samples (192 MS patients and 196 controls). Genotypes for <jats:italic>CYP2R1<\/jats:italic> (rs10741657, rs12794714), <jats:italic>CYP27B1<\/jats:italic> (rs10877012), <jats:italic>CYP24A1<\/jats:italic> (rs2248359), and <jats:italic>DBP<\/jats:italic> (rs7041, rs4588) were determined by PCR\/RFLP assay method. The study revealed a significant association with increased MS risk in SNPs rs10877012 (C\/A, <jats:italic><jats:italic>P\u2009=\u20090.0002<\/jats:italic><\/jats:italic>) of the <jats:italic>CYP27B1<\/jats:italic> gene and rs4588 (A\/A, <jats:italic><jats:italic>P\u2009=\u20090.04<\/jats:italic><\/jats:italic>) of the <jats:italic>DBP<\/jats:italic> gene. Additionally, the haplotypes of the <jats:italic>CYP2R1<\/jats:italic> gene revealed a significant association with MS patients and controls (GG, p\u2009=\u20091e-04; AA, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001). Moreover, only a SNP rs4588 of the <jats:italic>DBP<\/jats:italic> gene has been significantly associated (<jats:italic><jats:italic>P<\/jats:italic><\/jats:italic>\u2009=\u20090.04) with a clinical phenotype of multiple sclerosis and vitamin D deficiency. Understanding these genetic variations in multiple sclerosis susceptibility genes can help healthcare professionals improve early diagnosis and develop personalized treatment options.<\/jats:p>","DOI":"10.1371\/journal.pone.0333924","type":"journal-article","created":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T17:31:56Z","timestamp":1760117516000},"page":"e0333924","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Investigating genetic links of vitamin D metabolism pathway genes (CYP2R1, CYP27B1, CYP24A1, and DBP) in Multiple Sclerosis 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