{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T01:08:11Z","timestamp":1780708091115,"version":"3.54.1"},"reference-count":55,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T00:00:00Z","timestamp":1761523200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004442","name":"Narodowym Centrum Nauki","doi-asserted-by":"publisher","award":["UMO-2018\/30\/E\/NZ5\/00458"],"award-info":[{"award-number":["UMO-2018\/30\/E\/NZ5\/00458"]}],"id":[{"id":"10.13039\/501100004442","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Minister of Science under \u201cthe Regional Initiative of Excellence Program\u201d","award":["the Minister of Science under \u201cthe Regional Initiative of Excellence Program\u201d"],"award-info":[{"award-number":["the Minister of Science under \u201cthe Regional Initiative of Excellence Program\u201d"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Multiple evidence suggests that type 1 diabetes triggers perturbations in the nervous system both in human patients as well as in animal models of the disease. These perturbations are likely controlled by the expression of long non-coding RNAs (lncRNAs) and are present both in peripheral and central nervous system. To dissect the role of lncRNAs in diabetes-affected nervous system malfunctions, we conducted a comparative analysis of spinal cord transcriptome profiles between long-term (six months of duration) diabetic versus non-diabetic mice. The analysis of RNA sequencing data revealed that of 277 unique differentially expressed transcripts, 201 were up-regulated and 76 were down-regulated in the diabetic lumbar spinal cord. We also observed elevated expression of\n                    <jats:italic>\n                      <jats:italic>Snhg15<\/jats:italic>\n                    <\/jats:italic>\n                    lncRNA in diabetic spinal cord. The in-depth data analysis revealed differential expression of lncRNAs involved in the PI3K-Akt signaling pathway (KEGG: mmu04151) as well as substantial differences in several biological processes such as developmental process, cell communication, anatomical structure development and multicellular organismal process. Our analysis verified the role of lncRNAs in mouse spinal cord during the progression of type 1 diabetes and confirmed molecular alternations in the spinal cord occurring in the course of diabetic neuropathy.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0334245","type":"journal-article","created":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T17:57:11Z","timestamp":1761587831000},"page":"e0334245","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Novel insights into neuropathy: The impact of prolonged hyperglycemia on long non-coding RNA expression"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6577-7545","authenticated-orcid":true,"given":"Kamila","family":"Zglejc-Waszak","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan Pawe\u0142","family":"Jastrzebski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Wojtkiewicz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zenon","family":"Pidsudko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judyta Karolina","family":"Juranek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,10,27]]},"reference":[{"issue":"6","key":"pone.0334245.ref001","doi-asserted-by":"crossref","first-page":"5982","DOI":"10.1111\/ejn.15433","article-title":"The cross-talk between RAGE and DIAPH1 in neurological complications of diabetes: A review","volume":"54","author":"K Zglejc-Waszak","year":"2021","journal-title":"Eur J Neurosci"},{"issue":"10","key":"pone.0334245.ref002","doi-asserted-by":"crossref","first-page":"1642","DOI":"10.1111\/ejn.15991","article-title":"New insights into RAGE\/Diaph1 interaction as a modulator of actin cytoskeleton dynamics in peripheral nervous system in long-term hyperglycaemia","volume":"57","author":"K Zglejc-Waszak","year":"2023","journal-title":"Eur J Neurosci"},{"issue":"8","key":"pone.0334245.ref003","doi-asserted-by":"crossref","first-page":"1015","DOI":"10.1007\/s00109-023-02347-y","article-title":"Novel insights into the nervous system affected by prolonged hyperglycemia","volume":"101","author":"K Zglejc-Waszak","year":"2023","journal-title":"J Mol Med"},{"key":"pone.0334245.ref004","doi-asserted-by":"crossref","DOI":"10.4137\/BCI.S5340","article-title":"Morphological Changes and Immunohistochemical Expression of RAGE and its Ligands in the Sciatic Nerve of Hyperglycemic Pig (Sus Scrofa)","volume":"3","author":"JK Juranek","year":"2010","journal-title":"Biochem Insights"},{"issue":"3","key":"pone.0334245.ref005","doi-asserted-by":"crossref","first-page":"931","DOI":"10.2337\/db12-0632","article-title":"RAGE deficiency improves postinjury sciatic nerve regeneration in type 1 diabetic mice","volume":"62","author":"JK Juranek","year":"2013","journal-title":"Diabetes"},{"issue":"10","key":"pone.0334245.ref006","doi-asserted-by":"crossref","first-page":"1248","DOI":"10.1007\/s12264-022-00878-x","article-title":"Role of RAGE in the Pathogenesis of Neurological Disorders","volume":"38","author":"J Juranek","year":"2022","journal-title":"Neurosci Bull"},{"key":"pone.0334245.ref007","doi-asserted-by":"crossref","first-page":"27","DOI":"10.21037\/jxym.2020.02.03","article-title":"Involvement of the central nervous system in diabetic distal symmetrical polyneuropathy","volume":"6","author":"S Tesfaye","year":"2021","journal-title":"J Xiangya Med"},{"issue":"3","key":"pone.0334245.ref008","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1007\/s00726-021-02957-1","article-title":"Taurine ameliorates axonal damage in sciatic nerve of diabetic rats and high glucose exposed DRG neuron by PI3K\/Akt\/mTOR-dependent pathway","volume":"53","author":"M Zhang","year":"2021","journal-title":"Amino Acids"},{"issue":"9275","key":"pone.0334245.ref009","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/S0140-6736(00)05268-5","article-title":"Spinal-cord involvement in diabetic peripheral neuropathy","volume":"358","author":"SE Eaton","year":"2001","journal-title":"Lancet"},{"issue":"1","key":"pone.0334245.ref010","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1007\/BF01241031","article-title":"Pathological changes in the central and peripheral nervous system of young long-term diabetics. 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