{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T07:47:11Z","timestamp":1776844031796,"version":"3.51.2"},"reference-count":81,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T00:00:00Z","timestamp":1760659200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"AGRITECH European Union Next-GenerationEU","award":["1032 17\/06\/2022, CN00000022"],"award-info":[{"award-number":["1032 17\/06\/2022, CN00000022"]}]},{"DOI":"10.13039\/501100009886","name":"Regione Puglia","doi-asserted-by":"publisher","award":["1\/FSE\/2020 POR PUGLIA 2012\/2020"],"award-info":[{"award-number":["1\/FSE\/2020 POR PUGLIA 2012\/2020"]}],"id":[{"id":"10.13039\/501100009886","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>Predation on Aphrophoridae and other olive tree pests makes <jats:italic><jats:italic>Zelus renardii<\/jats:italic><\/jats:italic> a candidate for biocontrol actions to limit <jats:italic><jats:italic>Xylella fastidiosa<\/jats:italic><\/jats:italic> infections while mitigating other olive tree pests. The opportunity drives the search for an effective mass rearing method of <jats:italic><jats:italic>Z. renardii<\/jats:italic><\/jats:italic>. Predator rearing on artificial diets greatly benefits from feed-effective formulation, preparation, storage, preservation, and delivery. Given the several oligidic, meridic, and holidic available formulations, we face the challenge of a proper diet processing for delivery. To understand how to obtain a large number of preservable and sterile diet portions while avoiding microbial contamination, we explore prilling\/vibration techniques to rear <jats:italic><jats:italic>Z. renardii<\/jats:italic><\/jats:italic>. Prilling or vibrating the diets yields multicore microbeads or monocore microcapsules; water domains exist, whose arrangements are well-documented by the cryo-SEM study and represented in corresponding false-color images. Issues include the density interplay between low- or high-density alginate and the liquid diet formulation during prilling\/vibration. Other options relate to alginate stickiness or consistency, which makes it difficult to disperse the diet domains in the microbeads or to obtain a single diet domain per microcapsule because of unpredictable wall thickness and core lateralization. We suggest options to make microbeads and microcapsule portions available for up to one year for predators, stored in cold, pure water.<\/jats:p>","DOI":"10.1371\/journal.pone.0334859","type":"journal-article","created":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T17:28:53Z","timestamp":1760722133000},"page":"e0334859","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Microbeads and microcapsules for diet delivery to Zelus renardii"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7009-6891","authenticated-orcid":true,"given":"Ugo","family":"Picciotti","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppe 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Zimmerman","year":"1948"},{"issue":"3","key":"pone.0334859.ref002","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1093\/aesa\/79.3.535","article-title":"Genus Zelus Fabricius in the United States, Canada, and Northern Mexico (Hemiptera: Reduviidae)","volume":"79","author":"ER Hart","year":"1986","journal-title":"Annals of the Entomological Society of America"},{"key":"pone.0334859.ref003","first-page":"232","article-title":"Zelus renardii (Kolenati, 1856) (Insecta Heteroptera Reduviidae)","volume":"38","author":"P Dioli","year":"2013","journal-title":"Quad Studi Nat Romagna"},{"key":"pone.0334859.ref004","doi-asserted-by":"crossref","first-page":"31","DOI":"10.4081\/fe.2018.281","article-title":"Zelus renardii (Kolenati, 1856), a newly established alien species in Italy (Hemiptera: Reduviidae, Harpactorinae)","author":"M Pinzari","year":"2018","journal-title":"Fragm Entomol"},{"key":"pone.0334859.ref005","first-page":"421","article-title":"La chinche asesina Zelus renardii (Kolenati, 1856) (Heteroptera: Reduviidae) en Chile: comentarios despu\u00e9s de 15 a\u00f1os de su llegada al pa\u00eds","volume":"57","author":"EI Fa\u00fandez","year":"2015","journal-title":"Bol Soc Entomol Arag"},{"issue":"1","key":"pone.0334859.ref006","doi-asserted-by":"crossref","first-page":"32","DOI":"10.25085\/rsea.770106","article-title":"Zelus renardii (Hemiptera: Reduviidae: Harpactorinae: Harpactorini): first record from Argentina","volume":"77","author":"FE D\u2019Herv\u00e9","year":"2018","journal-title":"Rev Soc Entomol Arg"},{"key":"pone.0334859.ref007","first-page":"63","article-title":"Reporte para la provincia de Buenos Aires de tres especies de Heteroptera (Hemiptera) introducidas en Argentina","volume":"9","author":"DL Carpintero","year":"2019","journal-title":"Hist Nat"},{"key":"pone.0334859.ref008","first-page":"31","article-title":"On the presence of Zelus renardii Kolenati, 1857 (Hemiptera: Heteroptera: Reduviidae) in Jamaica","volume":"27","author":"T van der Heyden","year":"2023","journal-title":"Bol Mus Nac Hist Nat Parag"},{"issue":"02","key":"pone.0334859.ref009","doi-asserted-by":"crossref","DOI":"10.25085\/rsea.820210","article-title":"An update of the distribution of the assassin bug Zelus renardii (Heteroptera: Reduviidae) in Chile, with first records from the Oceanic Easter Island","volume":"82","author":"E Fa\u00fandez","year":"2023","journal-title":"Rev Soc Entomol Arg"},{"key":"pone.0334859.ref010","doi-asserted-by":"crossref","first-page":"327","DOI":"10.30550\/j.azl\/2110","article-title":"Nuevos datos de distribuci\u00f3n de la chinche asesina Zelus renardii (Kolenati, 1857) (Heteroptera: Reduviidae) en Sudam\u00e9rica y primera teratolog\u00eda conocida para la especie","author":"S Araya-Lobos","year":"2025","journal-title":"AZL"},{"issue":"2","key":"pone.0334859.ref011","doi-asserted-by":"crossref","first-page":"158","DOI":"10.3390\/insects13020158","article-title":"Zelus renardii Roaming in Southern 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species, candidate Xylella fastidiosa vectors","volume":"75","author":"N Lahbib","year":"2022","journal-title":"Bull Insectology"},{"issue":"2","key":"pone.0334859.ref016","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/j.ympev.2006.02.023","article-title":"Molecular phylogeny of silkmoths reveals the origin of domesticated silkmoth, Bombyx mori from Chinese Bombyx mandarina and paternal inheritance of Antheraea proylei mitochondrial DNA","volume":"40","author":"KP Arunkumar","year":"2006","journal-title":"Mol Phylogenet Evol"},{"issue":"1","key":"pone.0334859.ref017","first-page":"68","article-title":"Phylogeographic Relationships among Bombyx mandarina (Lepidoptera: Bombycidae) Populations and Their Relationships to B. mori Inferred from Mitochondrial Genomes","volume":"11","author":"M-J Kim","year":"2022","journal-title":"Biology 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Eng"},{"key":"pone.0334859.ref021","first-page":"1","article-title":"The IPM paradigm: concepts, strategies and tactics.","volume-title":"Integrated Pest Management: Concepts, Tactics, Strategies and Case Studies","author":"ME Gray","year":"2009"},{"key":"pone.0334859.ref022","doi-asserted-by":"crossref","first-page":"104095","DOI":"10.1016\/j.biocontrol.2019.104095","article-title":"Biological control and integrated pest management in organic and conventional systems","volume":"140","author":"BP Baker","year":"2020","journal-title":"Biological Control"},{"issue":"1","key":"pone.0334859.ref023","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1007\/s10526-017-9801-4","article-title":"Biological control using invertebrates and microorganisms: plenty of new opportunities","volume":"63","author":"JC van Lenteren","year":"2018","journal-title":"BioControl"},{"issue":"4","key":"pone.0334859.ref024","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1023\/A:1014193329979","article-title":"Suggestions for unifying the terminology in biological control","volume":"46","author":"J Eilenberg","year":"2001","journal-title":"BioControl"},{"issue":"1","key":"pone.0334859.ref025","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1007\/s10526-017-9831-y","article-title":"The status of biological control and recommendations for improving uptake for the future","volume":"63","author":"BIP Barratt","year":"2018","journal-title":"BioControl"},{"issue":"1","key":"pone.0334859.ref026","doi-asserted-by":"crossref","first-page":"8664","DOI":"10.1038\/s41598-019-45041-1","article-title":"Effectiveness of augmentative biological control depends on landscape context","volume":"9","author":"R Perez-Alvarez","year":"2019","journal-title":"Sci Rep"},{"issue":"2","key":"pone.0334859.ref027","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1023\/A:1022645210394","article-title":"Augmentative biological control of arthropods in Latin America","volume":"48","author":"JC Van Lenteren","year":"2003","journal-title":"BioControl"},{"key":"pone.0334859.ref028","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.biocontrol.2018.05.010","article-title":"Improving the efficiency of augmentative biological control with arthropod natural enemies: A modeling approach","volume":"125","author":"WN Plouvier","year":"2018","journal-title":"Biological Control"},{"key":"pone.0334859.ref029","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/B978-0-12-391453-8.00003-0","article-title":"Production of Heteropteran Predators","volume-title":"Mass Production of Beneficial Organisms","author":"P De Clercq","year":"2014"},{"key":"pone.0334859.ref030","first-page":"328","article-title":"Culture of entomophagous insects and their hosts.","volume-title":"Biological Control of Insects Pests and Weeds","author":"GL Finney","year":"1964"},{"key":"pone.0334859.ref031","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1079\/9780851996882.0115","article-title":"Comparison of artificially vs. Naturally reared natural enemies and their potential for use in biological control","volume-title":"Quality control and production of biological control agents: theory and testing procedures","author":"S Grenier","year":"2003"},{"key":"pone.0334859.ref032","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1007\/0-306-47585-5_20","article-title":"Mass production, storage, shipment and quality control of natural enemies.","volume-title":"Integrated Pest and Disease Management in Greenhouse Crops","author":"JC van Lenteren","year":"1999"},{"key":"pone.0334859.ref033","first-page":"173","article-title":"About the dependence of the growth of larvae of bacteria and enzymes and about variability and inheritance in the flesh flies","author":"EA Bogdanow","year":"1908","journal-title":"Arch Anat Physiol"},{"issue":"1","key":"pone.0334859.ref034","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1146\/annurev.en.01.010156.000313","article-title":"Insect Nutrition","volume":"1","author":"H Lipke","year":"1956","journal-title":"Annu Rev Entomol"},{"key":"pone.0334859.ref035","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1146\/annurev.ento.44.1.561","article-title":"Nutrition and culture of entomophagous insects","volume":"44","author":"SN Thompson","year":"1999","journal-title":"Annu Rev Entomol"},{"key":"pone.0334859.ref036","first-page":"53","article-title":"General Principles of Insect Nutritional Ecology","volume":"7","author":"H Gen\u00e7","year":"2006","journal-title":"Trakya Univ J Sci"},{"key":"pone.0334859.ref037","first-page":"175","article-title":"Breeding and maintaining high-quality insects.","volume-title":"Insects as Food and Feed: From Production to Consumption","author":"K Jensen","year":"2017"},{"key":"pone.0334859.ref038","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.cois.2017.07.014","article-title":"Using artificial diets to understand the nutritional physiology of Drosophila melanogaster","volume":"23","author":"MD Piper","year":"2017","journal-title":"Curr Opin Insect Sci"},{"issue":"8","key":"pone.0334859.ref039","doi-asserted-by":"crossref","first-page":"607","DOI":"10.3390\/insects15080607","article-title":"Performance of Artificial Diets for Zelus renardii (Hemiptera: Reduviidae) Rearing","volume":"15","author":"U Picciotti","year":"2024","journal-title":"Insects"},{"issue":"7","key":"pone.0334859.ref040","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1016\/j.jinsphys.2008.04.020","article-title":"Insect diets as mixtures: optimization for a polyphagous weevil","volume":"54","author":"SL Lapointe","year":"2008","journal-title":"J Insect Physiol"},{"key":"pone.0334859.ref041","volume-title":"Silkworm rearing","author":"P Wu","year":"1988"},{"issue":"4","key":"pone.0334859.ref042","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1093\/jee\/75.4.668","article-title":"Disposable Diet Packet for Feeding and Oviposition of Lygus hesperus (Hemiptera: Miridae)","volume":"75","author":"R Patana","year":"1982","journal-title":"Journal of Economic Entomology"},{"key":"pone.0334859.ref043","volume-title":"Rearing Lygus hesperus in the laboratory","author":"R Patana","year":"1985"},{"key":"pone.0334859.ref044","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1111\/j.1439-0418.1990.tb00023.x","article-title":"Rearing of Triatoma infestans Klug (Het., Reduviidae) in the absence of a live host","volume":"109","author":"JA N\u00fa\u00f1ez","year":"1990","journal-title":"J Applied Entomology"},{"issue":"4","key":"pone.0334859.ref045","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1603\/0013-8746(2005)098[0536:PADFRA]2.0.CO;2","article-title":"Pourable Artificial Diet for Rearing <I>Anoplophora glabripennis<\/I> (Coleoptera: Cerambycidae) and Methods to Optimize Larval Survival and Synchronize Development","volume":"98","author":"MA Keena","year":"2005","journal-title":"an"},{"key":"pone.0334859.ref046","doi-asserted-by":"crossref","DOI":"10.1201\/b18562","volume-title":"Insect diets: science and technology","author":"AC Cohen","year":"2015","edition":"2"},{"issue":"4","key":"pone.0334859.ref047","article-title":"Optimization an optimal artificial diet for the predatory bug Orius sauteri (hemiptera: anthocoridae)","volume":"8","author":"X-L Tan","year":"2013","journal-title":"PLoS One"},{"key":"pone.0334859.ref048","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1146\/annurev.bi.31.070162.003253","article-title":"Insect nutrition","volume":"31","author":"HL House","year":"1962","journal-title":"Annu Rev Biochem"},{"issue":"1","key":"pone.0334859.ref049","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1146\/annurev.en.22.010177.001445","article-title":"Quality Control in Mass Rearing","volume":"22","author":"DL Chambers","year":"1977","journal-title":"Annu Rev Entomol"},{"issue":"1","key":"pone.0334859.ref050","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1111\/j.1570-7458.1976.tb02614.x","article-title":"Sodium alginate as a gelling agent in diets for the cabbage looper, Trichoplusia ni","volume":"20","author":"NR Spencer","year":"1976","journal-title":"Entomologia Exp Applicata"},{"key":"pone.0334859.ref051","first-page":"4646","article-title":"An agar-free insect rearing artificial diet: a new approach for the low cost mass rearing of the Egyptian cotton leafworm, Spodoptera littoralis (Boisd.)(Lepidoptera: Noctuidae)","volume":"9","author":"AA Alfazairy","year":"2012","journal-title":"Life Sci J"},{"key":"pone.0334859.ref052","volume-title":"An abridged glossary of terms used in invertebrate pathology","author":"EA Steinhaus","year":"1970"},{"issue":"4","key":"pone.0334859.ref053","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1093\/ae\/40.4.240","article-title":"Microbial Contamination and Insect Rearing","volume":"40","author":"PP Sikorowski","year":"1994","journal-title":"American Entomologist"},{"issue":"39","key":"pone.0334859.ref054","doi-asserted-by":"crossref","DOI":"10.1002\/app.49177","article-title":"Preparation of slow release encapsulated insecticide and fertilizer based on superabsorbent polysaccharide microbeads","volume":"137","author":"PP Joshi","year":"2020","journal-title":"J of Applied Polymer Sci"},{"key":"pone.0334859.ref055","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1080\/87559129.2021.1874411","article-title":"Cheese Fortification: Review and Possible Improvements","volume":"38","author":"U Picciotti","year":"2022","journal-title":"Food Reviews International"},{"issue":"2","key":"pone.0334859.ref056","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1021\/bm049306e","article-title":"New hypothesis on the role of alternating sequences in calcium-alginate gels","volume":"6","author":"I Donati","year":"2005","journal-title":"Biomacromolecules"},{"issue":"5","key":"pone.0334859.ref057","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1093\/jee\/53.5.759","article-title":"A Wheat Germ Medium for Rearing the Pink Bollworm1","volume":"53","author":"PL Adkisson","year":"1960","journal-title":"Journal of Economic Entomology"},{"issue":"3","key":"pone.0334859.ref058","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1007\/BF03158318","article-title":"Rearing the egyptian cotton leafworm,Spodoptera littoralis, on a practical calcium-alginate diet","volume":"8","author":"A Navon","year":"1980","journal-title":"Phytoparasitica"},{"issue":"15","key":"pone.0334859.ref059","doi-asserted-by":"crossref","first-page":"4737","DOI":"10.1021\/jf0497511","article-title":"Evaluation of calcium-alginate gel as an artificial diet medium for bioassays on common cutworms","volume":"52","author":"M Morimoto","year":"2004","journal-title":"J Agric Food Chem"},{"issue":"5","key":"pone.0334859.ref060","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1093\/ee\/14.5.572","article-title":"Encapsulation of Steinernematid and Heterorhabditid Nematodes with Calcium Alginate: A New Approach for Insect Control and Other Applications","volume":"14","author":"HK Kaya","year":"1985","journal-title":"Environmental Entomology"},{"issue":"3","key":"pone.0334859.ref061","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1080\/09583159830225","article-title":"An Edible-to-insects Calcium Alginate Gel as a Carrier for Entomopathogenic Nematodes","volume":"8","author":"A Navon","year":"1998","journal-title":"Biocontrol Science and Technology"},{"issue":"4","key":"pone.0334859.ref062","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1007\/s10340-021-01349-4","article-title":"Calcium-alginate beads as a formulation for the application of entomopathogenic nematodes to control rootworms","volume":"94","author":"J Kim","year":"2021","journal-title":"J Pest Sci (2004)"},{"issue":"2","key":"pone.0334859.ref063","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1080\/0958315021000073439","article-title":"Microencapsulation: A Strategy for Formulation of Inoculum","volume":"13","author":"RS Winder","year":"2003","journal-title":"Biocontrol Science and Technology"},{"issue":"10","key":"pone.0334859.ref064","doi-asserted-by":"crossref","first-page":"1116","DOI":"10.1080\/09583157.2020.1793910","article-title":"Microencapsulation ofMeyerozyma guilliermondiiby spray drying using sodium alginate and soy protein isolate as wall materials: a biocontrol formulation for anthracnose disease of mango","volume":"30","author":"R L\u00f3pez-Cruz","year":"2020","journal-title":"Biocontrol Science and Technology"},{"key":"pone.0334859.ref065","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.clay.2015.02.001","article-title":"Encapsulation and characterization of slow-release microbial fertilizer from the composites of bentonite and alginate","author":"Y He","year":"2015","journal-title":"Applied Clay Science"},{"issue":"3","key":"pone.0334859.ref066","first-page":"138","article-title":"Industrially-Scalable Microencapsulation of Plant Beneficial Bacteria in Dry Cross-Linked Alginate Matrix","volume":"14","author":"SA Strobel","year":"2018","journal-title":"Ind Biotechnol (New Rochelle N Y)"},{"issue":"5","key":"pone.0334859.ref067","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.3390\/microorganisms13051123","article-title":"Jet-Breaking Extrusion of Alginate-Chitosan Capsules for Encapsulation of Plant 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prilling technique: Production of polynucleate alginate beads loading Serenoa Repens oil as intestinal delivery systems","volume":"599","author":"AA Lopedota","year":"2021","journal-title":"Int J Pharm"},{"key":"pone.0334859.ref071","doi-asserted-by":"crossref","first-page":"105974","DOI":"10.1016\/j.jddst.2024.105974","article-title":"Microbeads produced by prilling\/vibration technique: A new way to use polyvinyl alcohol in pediatric and veterinary formulations","volume":"99","author":"M Ivone","year":"2024","journal-title":"Journal of Drug Delivery Science and Technology"},{"key":"pone.0334859.ref072","doi-asserted-by":"crossref","first-page":"123762","DOI":"10.1016\/j.ijpharm.2023.123762","article-title":"Investigating the prilling\/vibration technique to produce gastric-directed drug delivery systems for misoprostol","volume":"651","author":"V D\u2019Amico","year":"2024","journal-title":"Int J Pharm"},{"key":"pone.0334859.ref073","first-page":"25","volume":"47","author":"R Roberto","year":"2016","journal-title":"Entomologica"},{"issue":"4","key":"pone.0334859.ref074","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1093\/jee\/72.4.579","article-title":"Mold Control in Insect Rearing Media: Survey of Agricultural Fungicides and Evaluation of the Use of Humectants12","volume":"72","author":"LR Ludemann","year":"1979","journal-title":"Journal of Economic Entomology"},{"key":"pone.0334859.ref075","first-page":"130","article-title":"Micro-organisms as contaminants and pathogens in insect rearing.","author":"M. Shapiro","year":"1984"},{"key":"pone.0334859.ref076","first-page":"115","article-title":"Pathogens and microbiological contaminants: their occurrence and control.","volume-title":"Boll Weevil Mass Rearing Technology","author":"PP. Sikorowski","year":"1984"},{"key":"pone.0334859.ref077","first-page":"289","article-title":"Problems with entomopathogens in insect rearing.","volume-title":"Epizootiology of Insect Diseases","author":"GG Soares","year":"1987"},{"issue":"5","key":"pone.0334859.ref078","doi-asserted-by":"crossref","first-page":"1441","DOI":"10.1093\/jee\/84.5.1441","article-title":"Effects of Bacterial Contamination on Gamma-Irradiated and Nonirradiated Corn Earworm (Lepidoptera: Noctuidae)","volume":"84","author":"PP Sikorowski","year":"1991","journal-title":"Journal of Economic Entomology"},{"key":"pone.0334859.ref079","volume-title":"Autoclave Sterilization: Principles, Loads and Exposure, Sterilization Controls","author":"ME Martignoni","year":"1978"},{"issue":"2","key":"pone.0334859.ref080","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/s11626-998-0094-x","article-title":"Culture of chondrocytes in alginate gel: variations in conditions of gelation influence the structure of the alginate gel, and the arrangement and morphology of proliferating chondrocytes","volume":"34","author":"MB Aydelotte","year":"1998","journal-title":"In Vitro Cell Dev Biol Anim"},{"issue":"8","key":"pone.0334859.ref081","doi-asserted-by":"crossref","first-page":"669","DOI":"10.3109\/02652048.2011.586068","article-title":"Microencapsulation using vibrating technology","volume":"28","author":"M Whelehan","year":"2011","journal-title":"J Microencapsul"}],"container-title":["PLOS One"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0334859","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T17:29:05Z","timestamp":1760722145000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0334859"}},"subtitle":[],"editor":[{"given":"Hon Fai","family":"Chan,","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2025,10,17]]},"references-count":81,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2025,10,17]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0334859","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,17]]}}}