{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:47:43Z","timestamp":1792802863933,"version":"4.5.0"},"reference-count":58,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:00:00Z","timestamp":1760572800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ubon Ratchathani University"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>Pancreatic cancer presents a significant therapeutic challenge characterized by poor survival rates, leading to development of innovative treatment strategies. This study evaluated the anti-cancer potential of 7-methoxyheptaphylline (7-MH), a carbazole alkaloid from <jats:italic><jats:italic>Clausena harmandiana<\/jats:italic><\/jats:italic>, against pancreatic cancer cells (PANC-1) and developed an environmentally sustainable extraction methodology using ultrasonic-assisted extraction (UAE). 7-MH demonstrated selective cytotoxicity against PANC-1 cells with preferential activity under nutrient deprivation conditions (PC<jats:sub>50<\/jats:sub>\u2009=\u20094.54 \u03bcM) compared to nutrient-rich conditions (IC<jats:sub>50<\/jats:sub>\u2009=\u200946.84 \u03bcM). This compound exhibited minimal toxicity toward normal MCE301 epithelial cells (IC<jats:sub>50<\/jats:sub>\u2009=\u200983.4 \u03bcM). Live-cell imaging showed dose-dependent apoptotic morphology including membrane blebbing and cell shrinkage within 24 hours. At concentrations of 25 and 50 \u03bcM, the compound significantly inhibited wound closure and colony formation, suggesting antimetastatic properties. Mechanistic analysis exhibited that 7-MH suppressed the phosphatidylinositol 3-kinase (PI3K)\/protein kinase B (Akt)\/mammalian target of rapamycin (mTOR) signaling pathway specially under nutrient deprived conditions. Western blot analysis showed 45% reduction in Akt expression, 43% decrease in mTOR phosphorylation, and complete inhibition of Akt phosphorylation at 20 \u00b5M concentration. For sustainable extraction of 7-MH, UAE using ethanol was optimized through response surface methodology (RSM) with central composite design (CCD). The optimal protocol (50 \u00b0C, 60 minutes, 0.40\u2009g\/10\u2009mL plant\/solvent ratio) achieved 1.26\u2009\u00b1\u20090.02% yield with only 3.55% deviation from predicted values. This developed extraction method provides an efficient and environmentally sustainable alternative to conventional halogenated solvent extraction methods. These findings offer valuable insights for advancing natural product-based cancer therapeutics and demonstrate the implementation of sustainable natural products extraction principles.<\/jats:p>","DOI":"10.1371\/journal.pone.0334901","type":"journal-article","created":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T17:45:22Z","timestamp":1760636722000},"page":"e0334901","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["Anti-cancer activity of 7-methoxyheptaphylline from Clausena harmandiana against PANC-1 pancreatic cancer cells and its sustainable extraction method"],"prefix":"10.1371","volume":"20","author":[{"given":"Juthamart","family":"Maneenet","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suresh","family":"Awale","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chantana","family":"Boonyarat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pornthip","family":"Waiwut","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4053-1899","authenticated-orcid":true,"given":"Rawiwun","family":"Kaewamatawong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,10,16]]},"reference":[{"issue":"5","key":"pone.0334901.ref001","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1053\/j.gastro.2023.02.012","article-title":"Pancreatic Cancer: Changing Epidemiology and New Approaches to Risk Assessment, Early Detection, and Prevention","volume":"164","author":"EM Stoffel","year":"2023","journal-title":"Gastroenterology"},{"issue":"4","key":"pone.0334901.ref002","doi-asserted-by":"crossref","DOI":"10.1001\/jamanetworkopen.2021.4708","article-title":"Estimated Projection of US Cancer Incidence and Death to 2040","volume":"4","author":"L Rahib","year":"2021","journal-title":"JAMA Netw Open"},{"issue":"32","key":"pone.0334901.ref003","doi-asserted-by":"crossref","first-page":"4698","DOI":"10.3748\/wjg.v28.i32.4698","article-title":"International patterns in incidence and mortality trends of pancreatic cancer in the last three decades: A joinpoint regression analysis","volume":"28","author":"I Ilic","year":"2022","journal-title":"World J Gastroenterol"},{"issue":"3","key":"pone.0334901.ref004","doi-asserted-by":"crossref","first-page":"1751","DOI":"10.1158\/0008-5472.CAN-05-3143","article-title":"Identification of arctigenin as an antitumor agent having the ability to eliminate the tolerance of cancer cells to nutrient starvation","volume":"66","author":"S Awale","year":"2006","journal-title":"Cancer Res"},{"issue":"5","key":"pone.0334901.ref005","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1002\/ijc.2910150505","article-title":"Establishment of a continuous tumor-cell line (panc-1) from a human carcinoma of the exocrine pancreas","volume":"15","author":"M Lieber","year":"1975","journal-title":"Int J Cancer"},{"issue":"4","key":"pone.0334901.ref006","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1097\/MPA.0b013e3181c15963","article-title":"Phenotype and genotype of pancreatic cancer cell lines","volume":"39","author":"EL Deer","year":"2010","journal-title":"Pancreas"},{"key":"pone.0334901.ref007","article-title":"Northeastern Thai traditional medicinal plants","author":"P Soralump","year":"2003","edition":"1"},{"issue":"5","key":"pone.0334901.ref008","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1007\/s12272-010-0505-x","article-title":"Biological activity of chemical constituents from Clausena harmandiana","volume":"33","author":"T Thongthoom","year":"2010","journal-title":"Arch Pharm Res"},{"issue":"2","key":"pone.0334901.ref009","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1021\/np100654m","article-title":"Claurailas A-D, cytotoxic carbazole alkaloids from the roots of Clausena harmandiana","volume":"74","author":"U Songsiang","year":"2011","journal-title":"J Nat Prod"},{"key":"pone.0334901.ref010","first-page":"159","article-title":"Analgesic, anti-inflammatory, antipyretic activities and acute toxicity of the ethanolic extract of Clausena harmandiana Pierre in animals","volume":"1","author":"J Wangboonskul","year":"2010","journal-title":"J Asian Assoc Sch Pharm"},{"issue":"3","key":"pone.0334901.ref011","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1055\/s-2000-8558","article-title":"Coumarins and carbazoles with antiplasmodial activity from Clausena harmandiana","volume":"66","author":"C Yenjai","year":"2000","journal-title":"Planta Med"},{"issue":"3","key":"pone.0334901.ref012","doi-asserted-by":"crossref","DOI":"10.1016\/j.diabres.2010.09.005","article-title":"Carbazoles and coumarins from Clausena harmandiana stimulate glucose uptake in L6 myotubes","volume":"90","author":"K Noipha","year":"2010","journal-title":"Diabetes Research and Clinical Practice"},{"issue":"6","key":"pone.0334901.ref013","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1016\/j.fitote.2012.04.026","article-title":"Antibacterial carbazole alkaloids from Clausena harmandiana twigs","volume":"83","author":"W Maneerat","year":"2012","journal-title":"Fitoterapia"},{"issue":"1","key":"pone.0334901.ref014","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1186\/s12906-022-03591-4","article-title":"Clausena Harmandiana root extract attenuated cognitive impairments via reducing amyloid accumulation and neuroinflammation in A\u03b21-42-induced rats","volume":"22","author":"N Nillert","year":"2022","journal-title":"BMC Complement Med Ther"},{"issue":"8","key":"pone.0334901.ref015","doi-asserted-by":"crossref","first-page":"3681","DOI":"10.3390\/cimb44080252","article-title":"Multifunctionality of Clausena harmandiana Extract and Its Active Constituents against Alzheimer\u2019s Disease","volume":"44","author":"C Boonyarat","year":"2022","journal-title":"Curr Issues Mol Biol"},{"issue":"9","key":"pone.0334901.ref016","doi-asserted-by":"crossref","first-page":"3755","DOI":"10.1016\/j.ejmech.2011.05.041","article-title":"Synthesis and cytotoxic activity of the heptaphylline and 7-methoxyheptaphylline series","volume":"46","author":"T Thongthoom","year":"2011","journal-title":"Eur J Med Chem"},{"issue":"8","key":"pone.0334901.ref017","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1248\/bpb.b23-00036","article-title":"7-Methoxyheptaphylline Enhances TRAIL-Induced Apoptosis of Colorectal Adenocarcinoma Cell via JNK-Mediated DR5 Expression","volume":"46","author":"C Boonyarat","year":"2023","journal-title":"Biol Pharm Bull"},{"issue":"1","key":"pone.0334901.ref018","doi-asserted-by":"crossref","first-page":"15","DOI":"10.3892\/or.2022.8452","article-title":"Neuroprotective and anticancer effects of 7\u2011Methoxyheptaphylline via the TAK1 pathway","volume":"49","author":"C Boonyarat","year":"2023","journal-title":"Oncol Rep"},{"issue":"1","key":"pone.0334901.ref019","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1039\/C5GC01008J","article-title":"CHEM21 selection guide of classical- and less classical-solvents","volume":"18","author":"D Prat","year":"2016","journal-title":"Green Chem"},{"key":"pone.0334901.ref020","author":"International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use","year":"2021"},{"key":"pone.0334901.ref021","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1016\/j.ultsonch.2016.06.035","article-title":"Ultrasound assisted extraction of food and natural products. Mechanisms, techniques, combinations, protocols and applications. A review","volume":"34","author":"F Chemat","year":"2017","journal-title":"Ultrason Sonochem"},{"issue":"14","key":"pone.0334901.ref022","doi-asserted-by":"crossref","first-page":"4869","DOI":"10.3390\/app10144869","article-title":"Extraction of Alkaloids Using Ultrasound from Pulp and By-Products of Soursop Fruit (Annona muricata L.)","volume":"10","author":"G Aguilar-Hern\u00e1ndez","year":"2020","journal-title":"Applied Sciences"},{"key":"pone.0334901.ref023","doi-asserted-by":"crossref","first-page":"463746","DOI":"10.1016\/j.chroma.2022.463746","article-title":"Effective extraction of bioactive alkaloids from the roots of Stephania tetrandra by deep eutectic solvents-based ultrasound-assisted extraction","volume":"1689","author":"Q He","year":"2023","journal-title":"J Chromatogr A"},{"key":"pone.0334901.ref024","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/j.foodchem.2013.06.136","article-title":"Optimization of ultrasonic-assisted extraction of bioactive alkaloid compounds from rhizoma coptidis (Coptis chinensis Franch.) using response surface methodology","volume":"142","author":"H Teng","year":"2014","journal-title":"Food Chemistry"},{"key":"pone.0334901.ref025","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1016\/j.crci.2017.09.004","article-title":"Optimization of ultrasound-assisted extraction of bioactive alkaloids from Stephania cambodica using response surface methodology","volume":"20","author":"C Dary","year":"2017","journal-title":"Comptes Rendus Chimie"},{"key":"pone.0334901.ref026","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1016\/j.cej.2011.06.039","article-title":"Ultrasound-assisted extraction of the three terpenoid indole alkaloids vindoline, catharanthine and vinblastine from Catharanthus roseus using ionic liquid aqueous solutions","volume":"172","author":"L Yang","year":"2011","journal-title":"Chemical Engineering Journal"},{"key":"pone.0334901.ref027","doi-asserted-by":"crossref","first-page":"1100150","DOI":"10.3389\/fchem.2023.1100150","article-title":"Simultaneous extraction and determination of alkaloids and organic acids in Uncariae Ramulas Cum Unicis by vortex-assisted matrix solid phase dispersion extraction coupled with UHPLC-MS\/MS","volume":"11","author":"X Xu","year":"2023","journal-title":"Front Chem"},{"issue":"4","key":"pone.0334901.ref028","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1002\/jps.3030450413","article-title":"Ultrasonic Extraction of Cinchona succirubra","volume":"45","author":"WF Head","year":"1956","journal-title":"Journal of the American Pharmaceutical Association (Scientific ed)"},{"issue":"2","key":"pone.0334901.ref029","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1007\/s11418-017-1165-7","article-title":"Enhanced ultrasound-assisted enzymatic hydrolysis extraction of quinolizidine alkaloids from Sophora alopecuroides L. seeds","volume":"72","author":"H Wang","year":"2018","journal-title":"J Nat Med"},{"key":"pone.0334901.ref030","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.chroma.2018.07.009","article-title":"Green techniques in comparison to conventional ones in the extraction of Amaryllidaceae alkaloids: Best solvents selection and parameters optimization","volume":"1567","author":"SS Takla","year":"2018","journal-title":"J Chromatogr A"},{"issue":"12","key":"pone.0334901.ref031","doi-asserted-by":"crossref","first-page":"3093","DOI":"10.1007\/s00216-016-9322-5","article-title":"Identification and determination of ergot alkaloids in Morning Glory cultivars","volume":"408","author":"J Nowak","year":"2016","journal-title":"Anal Bioanal Chem"},{"issue":"4","key":"pone.0334901.ref032","doi-asserted-by":"crossref","first-page":"1470","DOI":"10.1016\/j.ultsonch.2014.01.023","article-title":"Ultrasonic extraction of steroidal alkaloids from potato peel waste","volume":"21","author":"MB Hossain","year":"2014","journal-title":"Ultrason Sonochem"},{"issue":"19","key":"pone.0334901.ref033","first-page":"2466","article-title":"Chemical Constituents of Callistemon subulatus and Their Anti-Pancreatic Cancer Activity against Human PANC-1 Cell Line","volume":"11","author":"J Maneenet","year":"2022","journal-title":"Plants (Basel)"},{"issue":"9","key":"pone.0334901.ref034","doi-asserted-by":"crossref","first-page":"1967","DOI":"10.1016\/j.bmcl.2017.03.021","article-title":"Highly oxygenated antiausterity agents from the leaves of Uvaria dac","volume":"27","author":"S Awale","year":"2017","journal-title":"Bioorganic & Medicinal Chemistry Letters"},{"issue":"14","key":"pone.0334901.ref035","doi-asserted-by":"crossref","first-page":"1779","DOI":"10.1016\/j.bmcl.2019.05.010","article-title":"Design and synthesis of functionalized coumarins as potential anti-austerity agents that eliminates cancer cells\u2019 tolerance to nutrition starvation","volume":"29","author":"S Awale","year":"2019","journal-title":"Bioorg Med Chem Lett"},{"issue":"7","key":"pone.0334901.ref036","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25 years of image analysis","volume":"9","author":"CA Schneider","year":"2012","journal-title":"Nat Methods"},{"issue":"7","key":"pone.0334901.ref037","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"J Schindelin","year":"2012","journal-title":"Nat Methods"},{"key":"pone.0334901.ref038","unstructured":"ICH harmonised tripartite guideline: Validation of analytical procedures: Text and methodology Q2(R1). International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use. 2005. https:\/\/database.ich.org\/sites\/default\/files\/ICH_Q2-R2_Document_Step2_Guideline_2022_0324.pdf"},{"key":"pone.0334901.ref039","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1146\/annurev-pathmechdis-031621-024600","article-title":"Tumor Microenvironment in Pancreatic Cancer Pathogenesis and Therapeutic Resistance","volume":"18","author":"MH Sherman","year":"2023","journal-title":"Annu Rev Pathol"},{"key":"pone.0334901.ref040","doi-asserted-by":"crossref","first-page":"103749","DOI":"10.1016\/j.critrevonc.2022.103749","article-title":"Prospects of targeting PI3K\/AKT\/mTOR pathway in pancreatic cancer","volume":"176","author":"M Mortazavi","year":"2022","journal-title":"Crit Rev Oncol Hematol"},{"issue":"19","key":"pone.0334901.ref041","doi-asserted-by":"crossref","first-page":"10843","DOI":"10.3390\/ijms251910843","article-title":"Molecular Targets for the Diagnosis and Treatment of Pancreatic Cancer","volume":"25","author":"RG Pergolizzi","year":"2024","journal-title":"Int J Mol Sci"},{"issue":"12","key":"pone.0334901.ref042","doi-asserted-by":"crossref","first-page":"8054","DOI":"10.1021\/acs.jmedchem.3c00394","article-title":"Targeting Pancreatic Cancer with Novel Plumbagin Derivatives: Design, Synthesis, Molecular Mechanism, In Vitro and In Vivo Evaluation","volume":"66","author":"S Awale","year":"2023","journal-title":"J Med Chem"},{"issue":"16","key":"pone.0334901.ref043","doi-asserted-by":"crossref","first-page":"14313","DOI":"10.1021\/acs.jmedchem.4c01141","article-title":"Targeting Pancreatic Cancer with Novel Nicolaioidesin C Derivatives: Molecular Mechanism, In Vitro, and In Vivo Evaluations","volume":"67","author":"T Yamazaki","year":"2024","journal-title":"J Med Chem"},{"issue":"6","key":"pone.0334901.ref044","doi-asserted-by":"crossref","first-page":"1402","DOI":"10.1021\/acs.jnatprod.3c00019","article-title":"Nicolaioidesin C: An Antiausterity Agent Shows Promising Antitumor Activity in a Pancreatic Cancer Xenograft Mouse Model","volume":"86","author":"ND Phan","year":"2023","journal-title":"J Nat Prod"},{"issue":"85","key":"pone.0334901.ref045","doi-asserted-by":"crossref","first-page":"53740","DOI":"10.1039\/C7RA11200A","article-title":"Ancistrolikokines E\u2013H and related 5,8\u2032-coupled naphthylisoquinoline alkaloids from the Congolese lianaAncistrocladus likokowith antiausterity activities against PANC-1 human pancreatic cancer cells","volume":"7","author":"S Fayez","year":"2017","journal-title":"RSC Adv"},{"issue":"3","key":"pone.0334901.ref046","doi-asserted-by":"crossref","first-page":"87","DOI":"10.4103\/ctm.ctm_69_16","article-title":"Stemness-Related Markers in Cancer","volume":"3","author":"W Zhao","year":"2017","journal-title":"Cancer Transl Med"},{"issue":"6699","key":"pone.0334901.ref047","doi-asserted-by":"crossref","DOI":"10.1126\/science.adi7453","article-title":"Stem-cell states converge in multistage cutaneous squamous cell carcinoma development","volume":"384","author":"MA Taylor","year":"2024","journal-title":"Science"},{"issue":"2","key":"pone.0334901.ref048","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1038\/s41568-019-0216-7","article-title":"The PI3K-AKT network at the interface of oncogenic signalling and cancer metabolism","volume":"20","author":"G Hoxhaj","year":"2020","journal-title":"Nat Rev Cancer"},{"issue":"1","key":"pone.0334901.ref049","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1038\/s41392-021-00828-5","article-title":"Targeting PI3K\/Akt signal transduction for cancer therapy","volume":"6","author":"Y He","year":"2021","journal-title":"Signal Transduct Target Ther"},{"issue":"17","key":"pone.0334901.ref050","doi-asserted-by":"crossref","first-page":"10132","DOI":"10.3390\/ijms231710132","article-title":"Targeting PI3K\/AKT\/mTOR Signaling Pathway in Pancreatic Cancer: From Molecular to Clinical Aspects","volume":"23","author":"S Stanciu","year":"2022","journal-title":"Int J Mol Sci"},{"issue":"6","key":"pone.0334901.ref051","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1111\/j.1349-7006.2004.tb03247.x","article-title":"Kigamicin D, a novel anticancer agent based on a new anti-austerity strategy targeting cancer cells\u2019 tolerance to nutrient starvation","volume":"95","author":"J Lu","year":"2004","journal-title":"Cancer Sci"},{"key":"pone.0334901.ref052","doi-asserted-by":"crossref","first-page":"347","DOI":"10.3389\/fonc.2018.00347","article-title":"Sphere-Formation Assay: Three-Dimensional in vitro Culturing of Prostate Cancer Stem\/Progenitor Sphere-Forming Cells","volume":"8","author":"HF Bahmad","year":"2018","journal-title":"Front Oncol"},{"key":"pone.0334901.ref053","doi-asserted-by":"crossref","first-page":"113852","DOI":"10.1016\/j.addr.2021.113852","article-title":"Engineering strategies to capture the biological and biophysical tumor microenvironment in vitro","volume":"176","author":"ML Tan","year":"2021","journal-title":"Adv Drug Deliv Rev"},{"issue":"10","key":"pone.0334901.ref054","doi-asserted-by":"crossref","first-page":"732","DOI":"10.3390\/cells14100732","article-title":"Modeling Tumor Microenvironment Complexity In Vitro: Spheroids as Physiologically Relevant Tumor Models and Strategies for Their Analysis","volume":"14","author":"S Shah","year":"2025","journal-title":"Cells"},{"issue":"3","key":"pone.0334901.ref055","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1007\/s11771-010-0509-1","article-title":"Determination of contents of eight alkaloids in fruits of Macleaya cordata (Willd) R. Br. from different habitats and antioxidant activities of extracts","volume":"17","author":"M Zhong","year":"2010","journal-title":"J Cent South Univ Technol"},{"issue":"1","key":"pone.0334901.ref056","article-title":"Optimizing Ultrasonic Extraction and Purification of Nuciferine With Response Surface Method","volume":"19","author":"X Ren","year":"2024","journal-title":"Natural Product Communications"},{"key":"pone.0334901.ref057","doi-asserted-by":"crossref","first-page":"105851","DOI":"10.1016\/j.ultsonch.2021.105851","article-title":"Ultrasound-assisted extraction conditions optimisation using response surface methodology from Mitragyna speciosa (Korth.) Havil leaves","volume":"81","author":"F Zakaria","year":"2021","journal-title":"Ultrason Sonochem"},{"issue":"2","key":"pone.0334901.ref058","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1007\/s12393-011-9036-6","article-title":"Ultrasound-Assisted Extraction of Natural Products","volume":"3","author":"MD Esclapez","year":"2011","journal-title":"Food Eng Rev"}],"container-title":["PLOS One"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0334901","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T17:45:30Z","timestamp":1760636730000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0334901"}},"subtitle":[],"editor":[{"given":"Faham","family":"Khamesipour","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,10,16]]},"references-count":58,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2025,10,16]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0334901","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,16]]}}}