{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:03:03Z","timestamp":1787022183590,"version":"build-2736575974"},"reference-count":52,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T00:00:00Z","timestamp":1761782400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013274","name":"Institut National d'assurance Maladie-Invalidit\u00c3\u00a9","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013274","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["CM23\/00282"],"award-info":[{"award-number":["CM23\/00282"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Neglected tropical diseases (NTDs) are increasingly encountered in nonendemic settings due to migration, international travel, and global mobility. Their clinical management is often challenging: evidence from endemic regions is limited or not directly applicable, and healthcare providers are frequently unfamiliar with their diagnosis and treatment. Consequently, there is substantial heterogeneity in diagnostic workup, therapeutic choices, and follow-up strategies, which may affect patient outcomes and complicate guideline development. Given the sporadic presentation of these diseases across individual centers and the overall low caseload in nonendemic settings, conducting randomized controlled trials to define optimal strategies is largely unfeasible. To address these gaps, we designed Tropistry, a modular, multicenter registry that integrates harmonized data collection with embedded expert-informed clinical guidance.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Tropistry is an ambispective, multicenter registry planned for implementation across Belgian and European sites. Its modular structure will allow the sequential integration of disease-specific components, initially focusing on acute schistosomiasis and cutaneous\/mucocutaneous leishmaniasis (CL\/MCL), selected for their sporadic occurrence, clinical complexity, and lack of standardized management. Data will be collected through REDCap-based electronic case report forms aligned with up-to-date, evidence-based recommendations to ensure harmonized and comparable data collection. Embedded expert-informed guidance will support clinicians unfamiliar with these diseases, complemented by expedited access to ITM experts via TROPmail and aggregated feedback through an interactive R Shiny-based dashboard. A structured evaluation framework will assess feasibility, usability, data quality, user engagement, and treatment adherence to ensure the platform\u2019s utility and validity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Expected impact<\/jats:title>\n                    <jats:p>By centralizing harmonized data on diagnostic and therapeutic practices, Tropistry aims to address critical knowledge gaps in managing NTDs in nonendemic settings. For the initial targeted conditions, it will help clarify optimal strategies, such as the combination of corticosteroids and praziquantel for acute schistosomiasis and best practices for therapy and follow-up in CL\/MCL. Beyond data collection, Tropistry will provide embedded expert-informed guidance, interactive dashboards, and direct access to ITM experts to support clinicians and promote standardized care. Its modular design will enable expansion to other challenging NTDs, while the built-in evaluation framework ensures continuous assessment and optimization of feasibility, usability, and clinical utility. Ultimately, Tropistry seeks to strengthen collaboration between reference centers and non-specialized facilities and generate robust real-world evidence to inform clinical practice, guideline development, and future trials.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pone.0335032","type":"journal-article","created":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T17:37:48Z","timestamp":1761845868000},"page":"e0335032","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["Tropistry: A registry-based modular platform to improve care of neglected tropical diseases in nonendemic settings\u2014Study protocol with two targeted conditions: acute schistosomiasis and cutaneous\/mucocutaneous leishmaniasis"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0496-9508","authenticated-orcid":true,"given":"Carlos","family":"Bea-Serrano","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0604-1482","authenticated-orcid":true,"given":"Sami","family":"Alcedo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Berens-Riha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saskia","family":"van Henten","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johan","family":"van Griensven","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"Van Den Broucke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Soentjens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federico","family":"Gobbi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel","family":"Bottieau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,10,30]]},"reference":[{"key":"pone.0335032.ref001","unstructured":"World Health Organization. Ending the neglect to attain the Sustainable Development Goals: a road map for neglected tropical diseases 2021\u20132030. 2020."},{"issue":"1","key":"pone.0335032.ref002","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0005581","article-title":"The rise of neglected tropical diseases in the \u201cnew Texas\u201d","volume":"12","author":"PJ Hotez","year":"2018","journal-title":"PLoS Negl Trop Dis"},{"issue":"11","key":"pone.0335032.ref003","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0010885","article-title":"Human African trypanosomiasis cases diagnosed in non-endemic countries (2011-2020)","volume":"16","author":"JR Franco","year":"2022","journal-title":"PLoS Negl Trop Dis"},{"key":"pone.0335032.ref004","doi-asserted-by":"crossref","first-page":"102823","DOI":"10.1016\/j.tmaid.2025.102823","article-title":"Prevalence of neglected tropical diseases among migrants living in Europe: A systematic review and meta-analysis","volume":"64","author":"G Guido","year":"2025","journal-title":"Travel Med Infect Dis"},{"key":"pone.0335032.ref005","first-page":"23","volume-title":"UN Tourism. World Tourism Barometer 2025","author":"World Tourism Organization","year":"2025"},{"key":"pone.0335032.ref006","unstructured":"Migration to and from the EU - Statistics Explained - Eurostat. [cited 20 May 2025]. Available from: https:\/\/ec.europa.eu\/eurostat\/statistics-explained\/index.php?title=Migration_to_and_from_the_EU"},{"issue":"2","key":"pone.0335032.ref007","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1093\/trstmh\/traa192","article-title":"The impact of climate change on neglected tropical diseases: a systematic review","volume":"115","author":"R Tidman","year":"2021","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"pone.0335032.ref008","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.actatropica.2018.08.011","article-title":"Rabbit trypanosome detection in Phlebotomus perniciosus sand flies from the leishmaniasis outbreak in Madrid, Spain","volume":"187","author":"E Gonz\u00e1lez","year":"2018","journal-title":"Acta Trop"},{"key":"pone.0335032.ref009","doi-asserted-by":"crossref","first-page":"101036","DOI":"10.1016\/j.onehlt.2025.101036","article-title":"First autochthonous transmission of West Nile virus (WNV) lineage 2 to humans in 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Casulli","year":"2023","journal-title":"Parasitology"},{"issue":"11","key":"pone.0335032.ref021","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0004654","article-title":"Clinical Research in Neglected Tropical Diseases: The Challenge of Implementing Good Clinical (Laboratory) Practices","volume":"10","author":"R Ravinetto","year":"2016","journal-title":"PLoS Negl Trop Dis"},{"issue":"11","key":"pone.0335032.ref022","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0004736","article-title":"The Challenges of Conducting Clinical Research on Neglected Tropical Diseases in Remote Endemic Areas in Sudan","volume":"10","author":"S El-Safi","year":"2016","journal-title":"PLoS Negl Trop Dis"},{"key":"pone.0335032.ref023","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.zefq.2024.07.006","article-title":"Methodological approaches for generating robust evidence from trials in rare diseases","volume":"189","author":"R-D Hilgers","year":"2024","journal-title":"Z Evid Fortbild Qual Gesundheitswes"},{"issue":"10","key":"pone.0335032.ref024","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.1016\/j.cmi.2023.05.021","article-title":"Which trial do we need? A collaborative platform trial for cutaneous leishmaniasis amongst international travellers","volume":"29","author":"M Vandeputte","year":"2023","journal-title":"Clin Microbiol Infect"},{"key":"pone.0335032.ref025","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/j.ijid.2022.06.025","article-title":"Treatment outcome of imported cutaneous leishmaniasis among travelers and migrants infected with Leishmania major and Leishmania tropica: a retrospective study in European centers 2013 to 2019","volume":"122","author":"H Glans","year":"2022","journal-title":"Int J Infect Dis"},{"key":"pone.0335032.ref026","unstructured":"Medicines Agency E. Committee for Human Medicinal Products (CHMP) Guideline on registry-based studies Draft sent to the EU Regulatory Network for consultation including EMA committees, Patients\u2019 and Consumers\u2019 Working Party and Healthcare Professionals\u2019 Working Party. 2021 [cited 25 Apr 2025]. Available from: www.ema.europa.eu\/contact"},{"issue":"1","key":"pone.0335032.ref027","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/s15010-018-1241-2","article-title":"Neglected tropical diseases in Europe: rare diseases and orphan drugs?","volume":"47","author":"G Calleri","year":"2019","journal-title":"Infection"},{"key":"pone.0335032.ref028","first-page":"347","volume-title":"Evidence gaps for drugs and medical devices at market entry in Europe and potential solutions. Health Services Research (HSR)","author":"F Hulstaert","year":"2021"},{"issue":"5","key":"pone.0335032.ref029","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1111\/tmi.13048","article-title":"Eligibility criteria and outcome measures adopted in clinical trials of treatments of cutaneous leishmaniasis: systematic literature review covering the period 1991-2015","volume":"23","author":"L L\u00f3pez-Carvajal","year":"2018","journal-title":"Trop Med Int Health"},{"issue":"1","key":"pone.0335032.ref030","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1186\/s13071-020-04101-6","article-title":"The European Register of Cystic Echinococcosis, ERCE: state-of-the-art five years after its launch","volume":"13","author":"P Rossi","year":"2020","journal-title":"Parasit Vectors"},{"issue":"7","key":"pone.0335032.ref031","doi-asserted-by":"crossref","DOI":"10.1093\/jtm\/taae089","article-title":"Epidemiology of travel-associated dengue from 2007 to 2022: A GeoSentinel analysis","volume":"31","author":"A Duvignaud","year":"2024","journal-title":"J Travel Med"},{"issue":"11","key":"pone.0335032.ref032","first-page":"2381","article-title":"Analysis of Monkeypox Virus Exposures and Lesions by Anatomic Site","volume":"30","author":"SAJ Guagliardo","year":"2024","journal-title":"Emerg Infect Dis"},{"issue":"10","key":"pone.0335032.ref033","doi-asserted-by":"crossref","DOI":"10.1016\/S1473-3099(24)00080-X","article-title":"Consensus definitions in imported human schistosomiasis: a GeoSentinel and TropNet Delphi study","volume":"24","author":"F Tamarozzi","year":"2024","journal-title":"Lancet Infect Dis"},{"issue":"5","key":"pone.0335032.ref034","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/j.jinf.2005.07.022","article-title":"Imported Katayama fever: clinical and biological features at presentation and during treatment","volume":"52","author":"E Bottieau","year":"2006","journal-title":"J Infect"},{"issue":"10","key":"pone.0335032.ref035","doi-asserted-by":"crossref","first-page":"1693","DOI":"10.1093\/cid\/ciaa312","article-title":"Acute Schistosomiasis With a Schistosoma mattheei \u00d7 Schistosoma haematobium Hybrid Species in a Cluster of 34 Travelers Infected in South Africa","volume":"72","author":"L Cnops","year":"2021","journal-title":"Clin Infect Dis"},{"issue":"10","key":"pone.0335032.ref036","doi-asserted-by":"crossref","first-page":"1699","DOI":"10.1093\/cid\/ciaa319","article-title":"Acute Schistosomiasis: Which Molecular Diagnostic Test Is Best and Why","volume":"72","author":"JR Stothard","year":"2021","journal-title":"Clin Infect Dis"},{"issue":"1","key":"pone.0335032.ref037","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1186\/s13071-019-3442-7","article-title":"In vitro and in vivo activities of DW-3-15, a commercial praziquantel derivative, against Schistosoma japonicum","volume":"12","author":"X Wang","year":"2019","journal-title":"Parasit Vectors"},{"issue":"6","key":"pone.0335032.ref038","doi-asserted-by":"crossref","DOI":"10.1093\/jtm\/taaf065","article-title":"Acute schistosomiasis in travellers: outcomes of a short-course therapy","volume":"32","author":"J Clerinx","year":"2025","journal-title":"J Travel Med"},{"key":"pone.0335032.ref039","doi-asserted-by":"crossref","first-page":"101885","DOI":"10.1016\/j.tmaid.2020.101885","article-title":"Epidemiology, clinical pattern and impact of species-specific molecular diagnosis on management of leishmaniasis in Belgium, 2010-2018: A retrospective study","volume":"38","author":"M Vandeputte","year":"2020","journal-title":"Travel Med Infect Dis"},{"key":"pone.0335032.ref040","unstructured":"Centro Nacional de Epidemiolog\u00eda I de SCIC. Informe epidemiol\u00f3gico sobre la situaci\u00f3n de la leishmaniasis en Espa\u00f1a, 2023. Madrid; 2024 Jul. Available from: https:\/\/cne.isciii.es\/documents\/d\/cne\/informe_renave_leishmania-2023-1"},{"key":"pone.0335032.ref041","doi-asserted-by":"crossref","first-page":"102849","DOI":"10.1016\/j.tmaid.2025.102849","article-title":"Leishmaniasis in Syria - A call for action of the European Society for Clinical Microbiology and Infectious Diseases (ESCMID) Study Groups for Infections in Travellers and Migrants (ESGITM) and for Clinical Parasitology (ESGCP)","volume":"66","author":"A Abbara","year":"2025","journal-title":"Travel Med Infect Dis"},{"issue":"1","key":"pone.0335032.ref042","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1186\/s40249-017-0267-5","article-title":"Psychosocial impact of scars due to cutaneous leishmaniasis on high school students in Errachidia province, Morocco","volume":"6","author":"I Bennis","year":"2017","journal-title":"Infect Dis Poverty"},{"issue":"3","key":"pone.0335032.ref043","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0004495","article-title":"Treatment of Cutaneous Leishmaniasis Caused by Leishmania aethiopica: A Systematic Review","volume":"10","author":"J van Griensven","year":"2016","journal-title":"PLoS Negl Trop Dis"},{"issue":"2","key":"pone.0335032.ref044","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1111\/jtm.12089","article-title":"LeishMan recommendations for treatment of cutaneous and mucosal leishmaniasis in travelers, 2014","volume":"21","author":"J Blum","year":"2014","journal-title":"J Travel Med"},{"issue":"3","key":"pone.0335032.ref045","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0002130","article-title":"Methodology of clinical trials aimed at assessing interventions for cutaneous leishmaniasis","volume":"7","author":"P Olliaro","year":"2013","journal-title":"PLoS Negl Trop Dis"},{"issue":"8","key":"pone.0335032.ref046","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0012393","article-title":"Development of a Core Outcome Measure Instrument; 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