{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T18:40:14Z","timestamp":1764182414145,"version":"3.46.0"},"reference-count":58,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T00:00:00Z","timestamp":1764115200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["42106046"],"award-info":[{"award-number":["42106046"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Shanghai Science and Technology Commission","doi-asserted-by":"crossref","award":["23230760300"],"award-info":[{"award-number":["23230760300"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Microbial communities inhabiting intertidal sediment show diverse metabolisms to adapt to hydrodynamic conditions. However, the priming effect of exogenous organic matter on microbial metabolic fluxes remains poorly understood. Here we investigated microbial intercellular activities in surface sandy intertidal sediment (0\u20135\u2009cm depth, from the Nanhui tidal flat, East China Sea) under anoxic conditions by incubating with position-specific\n                    <jats:sup>13<\/jats:sup>\n                    C labelled glucose isotopologues. Elevated production of\n                    <jats:sup>13<\/jats:sup>\n                    C-CO\n                    <jats:sub>2<\/jats:sub>\n                    and phospholipid-derived fatty acids (PLFAs) was observed after 96-hour incubation, suggesting a positive priming effect on both catabolic and anabolic activities. The different incorporations of\n                    <jats:sup>13<\/jats:sup>\n                    C-label from glucose isotopologues into CO\n                    <jats:sub>2<\/jats:sub>\n                    and PLFAs reveal distinct intracellular carbon fluxes through the central metabolic network. Specifically, microbial communities preferentially utilize the Embden-Meyerhof-Parnas (EMP) pathway (62% flux), followed by the Pentose Phosphate (PP, 25%) and Entner-Doudoroff (ED, 13%) pathways. These flux distributions closely mirror their corresponding functional genome proportion (55% EMP, 26% PP, 20% ED), suggesting a metabolic balance between energy yield and enzyme cost across glycolytic routes. Furthermore, metabolic flux analysis based on\n                    <jats:sup>13<\/jats:sup>\n                    C-PLFA profiles highlights diverse metabolic strategies among different microbial taxa, primarily involving the EMP, ED and tricarboxylic acid pathways. This underscores the dual importance of energy production and carbon allocation for biomass synthesis. Given the high protein cost for the EMP pathway, energy acquisition may be prioritized by anaerobes to withstand the fluctuating conditions. Our results suggest that microbial interactions with intercellular networks may play a critical role in facilitating the priming and subsequent degradation of sedimentary organic matter.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0335053","type":"journal-article","created":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T18:37:40Z","timestamp":1764182260000},"page":"e0335053","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Glucose priming effect on microbial intercellular metabolic flux diversity in a marine intertidal 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