{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T17:33:04Z","timestamp":1761586384716,"version":"build-2065373602"},"reference-count":65,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T00:00:00Z","timestamp":1761523200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100020595","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["MOST 111-2320-B-038-040-MY3"],"award-info":[{"award-number":["MOST 111-2320-B-038-040-MY3"]}],"id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100020595","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["113-2628-B-038-009-MY3"],"award-info":[{"award-number":["113-2628-B-038-009-MY3"]}],"id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100020595","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["113-2321-B-255-001"],"award-info":[{"award-number":["113-2321-B-255-001"]}],"id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    <jats:italic>\n                      <jats:italic>Psidium cattleianum<\/jats:italic>\n                    <\/jats:italic>\n                    Sabine (Family Myrtaceae) is a Brazilian native shrub, valued for its diverse health and therapeutic attributes. The current study investigated the phytochemical profile along with the anticancer activities of the\n                    <jats:italic>\n                      <jats:italic>n-<\/jats:italic>\n                    <\/jats:italic>\n                    hexane extract (HE) of\n                    <jats:italic>\n                      <jats:italic>P. cattleianum<\/jats:italic>\n                    <\/jats:italic>\n                    aerial parts and its subfractions. GC-MS and HPTLC-MS were used for phytochemical analysis. The human breast adenocarcinoma cells (MCF-7) and the human colon cancer cells (HCT-116) were used to investigate the anticancer effect in the viability, migration, and clonogenic assays. The GC-MS analysis of HE identified thirty-two components categorized mainly into terpenes, hydrocarbons, and sterols.\n                    <jats:italic>\u03b2<\/jats:italic>\n                    -caryophyllene oxide (12.07%) and humulene (7.42%) were the most abundant oxygenated and non-oxygenated metabolites, respectively. Concerning HE\u2019s subfractions, fraction I is prolific with caryophyllene oxide (19.48%) and humulene (9.96%), while fraction II was rich in caryophyllene oxide (6.89%). HPTLC-MS analysis of fractions III-V identified the presence of nonadecatetraene, heptacosanol, and dihydroxy-oxo-ursenoic acid in fraction III; caryophyllene and littordial C in fraction IV, while guavanoic acid,\n                    <jats:italic>\n                      <jats:italic>p<\/jats:italic>\n                    <\/jats:italic>\n                    -coumaroyl caffeoylquinic acid, cholestane heptol, tocopherol, heptacosanedione, and\n                    <jats:italic>\n                      <jats:italic>trans<\/jats:italic>\n                    <\/jats:italic>\n                    -calamenene in fraction V. Concerning the anticancer results, the HE showed potent cytotoxicity with IC\n                    <jats:sub>50<\/jats:sub>\n                    29.18\u2009\u00b1\u20090.43 \u03bcg\/mL (MCF-7) and 56.55\u2009\u00b1\u20096.8 \u03bcg\/mL (HCT-116). In addition, at maximum tested doses approximating \u00bd IC\n                    <jats:sub>50<\/jats:sub>\n                    (15 and 28 \u03bcg\/mL) in cytotoxicity assay, it displayed significant percent wound closure of 22.78\u2009\u00b1\u20092.13% and 12.76\u2009\u00b1\u20091.88%, respectively. While at doses corresponding to \u00bc IC\n                    <jats:sub>50<\/jats:sub>\n                    (7.5 and 14 \u03bcg\/mL), the HE displayed a colony formation efficiency of 2% and 0% on MCF-7 and HCT-116, respectively. Subfractions I and II, rich in caryophyllane sesquiterpenes, such as caryophyllene oxide, showed the best activity in all assays. Molecular docking of\n                    <jats:italic>\u03b2<\/jats:italic>\n                    -caryophyllene oxide, as the most identified bioactive metabolite, revealed an energetically favorable binding pose driven through hydrophobic interactions at the estrogen receptor ligand binding domain. The study endorses\n                    <jats:italic>\n                      <jats:italic>P. cattleianum<\/jats:italic>\n                    <\/jats:italic>\n                    HE and its selected fractions in the control of breast and colon cancers; however, further investigation into an appropriate\n                    <jats:italic>\n                      <jats:italic>in vivo<\/jats:italic>\n                    <\/jats:italic>\n                    model is required.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0335134","type":"journal-article","created":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T17:29:43Z","timestamp":1761586183000},"page":"e0335134","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phytochemical characterization and anticancer potential of Psidium cattleianum Sabine aerial parts\u2019 n-hexane extract and its subfractions"],"prefix":"10.1371","volume":"20","author":[{"given":"Eman M.","family":"El-Deeb","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatma A.","family":"Moharram","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hussein S.","family":"Taha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed R.","family":"Elgindi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7220-6356","authenticated-orcid":true,"given":"Kuei-Hung","family":"Lai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9057-4582","authenticated-orcid":true,"given":"Hassan Y.","family":"Ebrahim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heba E.","family":"Elsayed","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2025,10,27]]},"reference":[{"issue":"3","key":"pone.0335134.ref001","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1038\/s41573-020-00114-z","article-title":"Natural products in drug discovery: advances and opportunities","volume":"20","author":"International Natural Product Sciences Taskforce","year":"2021","journal-title":"Nat Rev Drug Discov"},{"issue":"3","key":"pone.0335134.ref002","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.drudis.2013.10.013","article-title":"Natural products as lead structures: chemical transformations to create lead-like libraries","volume":"19","author":"M Pascolutti","year":"2014","journal-title":"Drug Discov 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