{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T17:57:06Z","timestamp":1791395826926,"version":"4.3.3"},"reference-count":86,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["OPP1185057"],"award-info":[{"award-number":["OPP1185057"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["CIHR156307"],"award-info":[{"award-number":["CIHR156307"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Globally, almost half of all early childhood deaths are linked to severe undernutrition, herein referred to as severe malnutrition. Mortality in severely malnourished children is often attributed to common infectious diseases, including enteric infections. It has been proposed that impaired intestinal barrier function contributes to mortality, but direct evidence is limited and thus there exists a need to develop improved preclinical models to test these and other mechanistic hypotheses. In this study, we aimed to describe differences in response to enteric inflammation and infection in the colon of malnourished mice compared to well-nourished littermates. C57Bl\/6 male weanlings were fed isocaloric diets, either a low 1% protein diet (LPD) or a control 18% protein diet (CPD) for 2-weeks either in combination with oral administration of dextran sodium sulfate (DSS), or\n                    <jats:italic>Citrobacter rodentium<\/jats:italic>\n                    (\n                    <jats:italic>C. rodentium)<\/jats:italic>\n                    . LPD-fed mice were more susceptible to DSS or\n                    <jats:italic>C. rodentium<\/jats:italic>\n                    as evidenced by increased clinical severity scores, and reaching their humane endpoints. LPD-fed mice also showed more signs of colonic dysfunction with reduced levels of tight junction proteins, higher colonic pathogen load, and increased systemic inflammation and bacterial spread. Taken together, these observations show that malnourished animals have increased susceptibility to intestinal dysfunction caused by either chemical exposure or infection. These novel preclinical models can be used to further elucidate the processes involved in enteric dysfunction in malnutrition and to test therapies to improve intestinal repair and outcomes.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0335322","type":"journal-article","created":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T17:24:21Z","timestamp":1791393861000},"page":"e0335322","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Feeding a low-protein diet exacerbates the susceptibility to Citrobacter rodentium and dextran sulfate sodium-induced intestinal injury in mice"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-2020-302X","authenticated-orcid":true,"given":"Mehakpreet K.","family":"Thind","sequence":"first","affiliation":[{"name":"University of Toronto","place":["Toronto, Ontario, Canada"],"department":["Department of Nutritional Sciences, Faculty of Medicine"]},{"name":"Translational Medicine Program, The Hospital for Sick Children","place":["Toronto, Ontario, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary","family":"Flanagan","sequence":"additional","affiliation":[{"name":"Translational Medicine Program, The Hospital for Sick Children","place":["Toronto, Ontario, Canada"]},{"name":"The Hospital for Sick Children","place":["Toronto, Ontario, Canada"],"department":["Division of Gastroenterology, Hepatology and Nutrition"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanne","family":"de Wit","sequence":"additional","affiliation":[{"name":"University Medical Center Groningen","place":["Groningen, Netherlands"],"department":["Department of Cardiology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aida","family":"Glembocki","sequence":"additional","affiliation":[{"name":"Western University and London Health Sciences Centre","place":["London, Ontario, Canada"],"department":["Department of Pathology and Laboratory Medicine"]},{"name":"Child Health Evaluative Sciences, The Hospital for Sick Children, Peter Gilgan Centre for Research and Learning","place":["Toronto, Ontario, Canada"]},{"name":"University of Toronto","place":["Toronto, Canada"],"department":["Institute of Health Policy, Management and Evaluation"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-1132-8933","authenticated-orcid":true,"given":"Jiali","family":"Pan","sequence":"additional","affiliation":[{"name":"University of Toronto","place":["Toronto, Ontario, Canada"],"department":["Department of Nutritional Sciences, Faculty of Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celine","family":"Cuinat","sequence":"additional","affiliation":[{"name":"University of Toronto","place":["Toronto, Ontario, Canada"],"department":["Department of Nutritional Sciences, Faculty of Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon","family":"Leung","sequence":"additional","affiliation":[{"name":"Cell Biology Program, The Hospital for Sick Children","place":["Toronto, Ontario, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1732-4242","authenticated-orcid":true,"given":"Shamsun Nahar","family":"Shaima","sequence":"additional","affiliation":[{"name":"University of Toronto","place":["Toronto, Ontario, Canada"],"department":["Department of Nutritional Sciences, Faculty of Medicine"]},{"name":"Translational Medicine Program, The Hospital for Sick Children","place":["Toronto, Ontario, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamza","family":"Sghayare","sequence":"additional","affiliation":[{"name":"Translational Medicine Program, The Hospital for Sick Children","place":["Toronto, Ontario, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-3807-010X","authenticated-orcid":true,"given":"Kinza","family":"Usmani","sequence":"additional","affiliation":[{"name":"Translational Medicine Program, The Hospital for Sick Children","place":["Toronto, Ontario, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mishaal","family":"Munawar","sequence":"additional","affiliation":[{"name":"Cell Biology Program, The Hospital for Sick Children","place":["Toronto, Ontario, Canada"]},{"name":"University of Toronto","place":["Toronto, Ontario, Canada"],"department":["Department of Biochemistry, Faculty of Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Kim","sequence":"additional","affiliation":[{"name":"Cell Biology Program, The Hospital for Sick Children","place":["Toronto, Ontario, Canada"]},{"name":"University of Toronto","place":["Toronto, Ontario, Canada"],"department":["Department of Biochemistry, Faculty of Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena M.","family":"Comelli","sequence":"additional","affiliation":[{"name":"University of Toronto","place":["Toronto, Ontario, Canada"],"department":["Department of Nutritional Sciences, Faculty of Medicine"]},{"name":"University of Toronto","place":["Toronto, Ontario, Canada"],"department":["Centre for Child Nutrition and Health, Faculty of Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amber","family":"Farooqui","sequence":"additional","affiliation":[{"name":"Translational Medicine Program, The Hospital for Sick Children","place":["Toronto, Ontario, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celine","family":"Bourdon","sequence":"additional","affiliation":[{"name":"Translational Medicine Program, The Hospital for Sick Children","place":["Toronto, Ontario, 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J.","family":"Bandsma","sequence":"additional","affiliation":[{"name":"University of Toronto","place":["Toronto, Ontario, Canada"],"department":["Department of Nutritional Sciences, Faculty of Medicine"]},{"name":"Translational Medicine Program, The Hospital for Sick Children","place":["Toronto, Ontario, Canada"]},{"name":"The Hospital for Sick Children","place":["Toronto, Ontario, Canada"],"department":["Division of Gastroenterology, Hepatology and Nutrition"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,10,7]]},"reference":[{"issue":"5","key":"pone.0335322.ref001","article-title":"Childhood mortality during and after acute illness in Africa and south Asia: a prospective cohort study","volume":"10","author":"Childhood Acute Illness and Nutrition (CHAIN) Network","year":"2022","journal-title":"Lancet Glob Health"},{"key":"pone.0335322.ref002","article-title":"Immune dysfunction as a cause and consequence of 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