{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T01:26:44Z","timestamp":1788830804779,"version":"build-2803163510"},"reference-count":70,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T00:00:00Z","timestamp":1764806400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fundamental Research Program of Shanxi Province","award":["202403021221208"],"award-info":[{"award-number":["202403021221208"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Autoimmune hepatitis (AIH) is a complex immune-mediated liver disorder characterized by dysregulated immune responses. This study aimed to decode the distinct immune landscape and regulatory mechanisms of AIH using integrated single-cell and bulk RNA sequencing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The data of single-cell RNA-seq (scRNA-seq) and bulk RNA-seq were downloaded from GEO database. The cell clustering, differential gene expression, trajectory analysis, functional enrichment, and cell-cell communication were performed were analyzed using R software.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Five major immune cell types were identified, with CD8\u2009+\u2009T cells and NK cells significantly expanded in AIH. Functional enrichment showed upregulation of immune activation, inflammation, and metabolic pathways in these cells. The cell-cell communication analysis revealed robust interactions between CD8\u2009+\u2009T and NK cells, primarily driven by the CCL5-CCR signaling axis. Integrative analysis of scRNA-seq and bulk RNA-seq identified four common DEIRGs (ITK, IL7R, CXCR4 and SORT1) and one transcription factor PRDM1.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study identified dysregulated immune cell clusters, signaling pathways, and potential therapeutic targets in AIH.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pone.0335605","type":"journal-article","created":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T18:28:26Z","timestamp":1764872906000},"page":"e0335605","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["Decoding the distinct immune landscape and possible regulatory mechanisms of autoimmune hepatitis through integrated single-cell and bulk RNA 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