{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T18:02:36Z","timestamp":1783533756252,"version":"3.55.0"},"reference-count":51,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T00:00:00Z","timestamp":1761868800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Iron (Fe) deficiency is a major nutritional stress affecting plant growth and metabolism. This study was conducted on three\n                    <jats:italic>\n                      <jats:italic>Medicago truncatula<\/jats:italic>\n                    <\/jats:italic>\n                    genotypes (TN8.20 and A17 as tolerant and T1.11 as a sensible genotype) cultivated in optimal and Fe-deficient conditions. Assessment of Fe deficiency effects was performed on some physiological and biochemical parameters with a particular focus on superoxide dismutase (SOD) activities and genes expression in roots. Our data showed that the sensitive genotype TN1.11 was more affected by Fe starvation compared to A17 and TN8.20. Overall, the relatively higher tolerance of A17 and TN8.20 to Fe deficiency was positively correlated to their ability to maintain higher plant biomass, Fe content, Fe use efficiency, Cu and Zn contents in roots. The oxidative stress associated with Fe-deficiency was evidenced by increased roots hydrogen peroxide (H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    ) levels, especially in TN1.11 genotype. In contrast, Assessment of SOD activity in roots revealed a significant increase in Cu\/ZnSOD and MnSOD activities under Fe-deficient conditions, particularly in TN8.20. Gene expression analysis showed differential regulation of\n                    <jats:italic>\n                      <jats:italic>FeSOD<\/jats:italic>\n                    <\/jats:italic>\n                    ,\n                    <jats:italic>\n                      <jats:italic>Cu\/ZnSOD<\/jats:italic>\n                    <\/jats:italic>\n                    and\n                    <jats:italic>CHSOD<\/jats:italic>\n                    genes in response to Fe deficiency. Notably, TN8.20 exhibited upregulation of\n                    <jats:italic>\n                      <jats:italic>Cu\/ZnSOD<\/jats:italic>\n                    <\/jats:italic>\n                    and down regulation of\n                    <jats:italic>CHSOD<\/jats:italic>\n                    under Fe-deficient conditions. TN8.20, which showed the highest SOD activities and gene expression levels, was identified as the most tolerant genotype. These findings highlight the physiological and molecular responses of\n                    <jats:italic>\n                      <jats:italic>Medicago truncatula<\/jats:italic>\n                    <\/jats:italic>\n                    to Fe deficiency and emphasize the comparative leaf-root analyses, revealing that SOD related genes in roots may serve as useful molecular markers for selecting Fe-deficiency-tolerant genotypes to cope with oxidative stress and nutrient imbalances.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0335634","type":"journal-article","created":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T17:24:33Z","timestamp":1761931473000},"page":"e0335634","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Biochemical and molecular assessment of the superoxide dismutase (SOD) antioxidant enzyme regulation within Medicago truncatula root in response to iron deficiency"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-8031-4225","authenticated-orcid":true,"given":"Nadia","family":"Kallala","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wissal","family":"M'sehli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7575-4817","authenticated-orcid":true,"given":"Manel","family":"Chaouachi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karima","family":"Jalali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghassen","family":"Abid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haythem","family":"Mhadhbi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,10,31]]},"reference":[{"issue":"6","key":"pone.0335634.ref001","doi-asserted-by":"crossref","first-page":"5194","DOI":"10.3390\/cimb46060312","article-title":"Soil 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Arabidopsis","volume":"26","author":"Y Xu","year":"2025","journal-title":"Int J Mol Sci"},{"key":"pone.0335634.ref008","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/j.plaphy.2022.11.024","article-title":"Iron counteracts zinc-induced toxicity in soybeans","volume":"194","author":"NT de Oliveira","year":"2023","journal-title":"Plant Physiol Biochem"},{"issue":"10","key":"pone.0335634.ref009","doi-asserted-by":"crossref","first-page":"1556","DOI":"10.3390\/antiox10101556","article-title":"Nitric oxide prevents Fe deficiency-induced photosynthetic disturbance, and oxidative stress in alfalfa by regulating Fe acquisition and antioxidant defense","volume":"10","author":"MA Rahman","year":"2021","journal-title":"Antioxidants (Basel)"},{"issue":"3","key":"pone.0335634.ref010","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1111\/jipb.13601","article-title":"Reactive oxygen species: multidimensional regulators of plant adaptation to abiotic stress and development","volume":"66","author":"P Wang","year":"2024","journal-title":"J Integr Plant Biol"},{"issue":"5","key":"pone.0335634.ref011","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.1007\/s00204-024-03696-4","article-title":"Several lines of antioxidant defense against oxidative stress: antioxidant enzymes, nanomaterials with multiple enzyme-mimicking activities, and low-molecular-weight antioxidants","volume":"98","author":"K Jomova","year":"2024","journal-title":"Arch Toxicol"},{"issue":"1","key":"pone.0335634.ref012","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1007\/s12298-021-00926-2","article-title":"Comparative genomic analysis of superoxide dismutase (SOD) genes in three Rosaceae species and expression analysis in Pyrus bretschneideri","volume":"27","author":"G Li","year":"2021","journal-title":"Physiol Mol Biol Plants"},{"issue":"9","key":"pone.0335634.ref013","first-page":"348","article-title":"Understanding the role of the antioxidant system and 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Sci"},{"key":"pone.0335634.ref022","first-page":"871","article-title":"Nodule permeability to O2 and nitrogenase-linked respiration in bean genotypes varying in the tolerance of N2 fixation to P deficiency","volume":"34","author":"V Vadez","year":"1996","journal-title":"Plant Physiol Biochem"},{"issue":"3","key":"pone.0335634.ref023","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1111\/1442-1984.12245","article-title":"Biodiversity within Medicago truncatula genotypes toward response to iron deficiency: Investigation of main tolerance mechanisms","volume":"34","author":"N Kallala","year":"2019","journal-title":"Plant Species Biol"},{"issue":"5","key":"pone.0335634.ref024","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1111\/j.1365-3040.1997.00097.x","article-title":"Elongation by primary lateral roots and adventitious roots during conditions of hypoxia and high ethylene concentrations","volume":"20","author":"EJW Visser","year":"1997","journal-title":"Plant Cell 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(Fabaceae): relevance to the iron nutrition of developing seeds","volume":"197","author":"M Grusak","year":"1995","journal-title":"Planta"},{"key":"pone.0335634.ref026","doi-asserted-by":"crossref","first-page":"805247","DOI":"10.3389\/fpls.2022.805247","article-title":"Identification of quantitative trait loci associated with iron deficiency tolerance in Maize","volume":"13","author":"J Xu","year":"2022","journal-title":"Front Plant Sci"},{"key":"pone.0335634.ref027","first-page":"336","article-title":"Responses of two forage legumes to direct iron deficiency: a comparative study","volume":"18","author":"W M\u2019Sehli-Bouftira","year":"2012","journal-title":"J Biol Res"},{"issue":"1","key":"pone.0335634.ref028","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/S0168-9452(99)00197-1","article-title":"Oxidative stress and some antioxidant systems in acid rain-treated bean plants","volume":"151","author":"V Velikova","year":"2000","journal-title":"Plant Science"},{"key":"pone.0335634.ref029","first-page":"248","article-title":"Determinacion de proteinas: metodo de Bradford","volume":"72","author":"MM Bradford","year":"1976","journal-title":"Anal Biochem"},{"issue":"2","key":"pone.0335634.ref030","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1007\/BF02670468","article-title":"A simple and efficient method for isolating RNA from pine trees","volume":"11","author":"S Chang","year":"1993","journal-title":"Plant Mol Biol Rep"},{"issue":"2","key":"pone.0335634.ref031","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1007\/s11240-014-0707-x","article-title":"Identification and characterization of drought stress responsive genes in faba bean (Vicia faba L.) by suppression subtractive hybridization","volume":"121","author":"G Abid","year":"2015","journal-title":"Plant Cell Tiss Organ 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architecture","volume":"10","author":"PR Divte","year":"2021","journal-title":"Agric Res"},{"issue":"2","key":"pone.0335634.ref035","doi-asserted-by":"crossref","first-page":"31","DOI":"10.3390\/plants8020031","article-title":"Tolerance of iron-deficient and -toxic soil conditions in rice","volume":"8","author":"A Mahender","year":"2019","journal-title":"Plants"},{"key":"pone.0335634.ref036","doi-asserted-by":"crossref","first-page":"1204836","DOI":"10.3389\/fpls.2023.1204836","article-title":"Silicon mitigates iron deficiency in two energy cane cultivars by modulating physiological and nutritional mechanisms","volume":"14","author":"GCM Teixeira","year":"2023","journal-title":"Front Plant Sci"},{"key":"pone.0335634.ref037","doi-asserted-by":"crossref","first-page":"663477","DOI":"10.3389\/fpls.2021.663477","article-title":"Cross-talks between macro- and micronutrient uptake and signaling in plants","volume":"12","author":"X Fan","year":"2021","journal-title":"Front Plant 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Springer Nature Singapore"},{"key":"pone.0335634.ref043","doi-asserted-by":"crossref","first-page":"2058","DOI":"10.1007\/s00344-024-11249-3","article-title":"Elevated expression of YSL genes and enhanced physiological responses in rice subjected to iron application","volume":"43","author":"A Saleem","year":"2024","journal-title":"J Plant Growth Regul"},{"issue":"3","key":"pone.0335634.ref044","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1007\/s10725-021-00716-6","article-title":"Antioxidant regulation of iron as a repressor for salt-induced leaf senescence in perennial grass species","volume":"94","author":"Y Wang","year":"2021","journal-title":"Plant Growth Regul"},{"issue":"5","key":"pone.0335634.ref045","doi-asserted-by":"crossref","DOI":"10.1111\/ppl.14026","article-title":"Priming seeds with salicylic acid modulates membrane integrity, antioxidant defense, and gene expression in Medicago sativa grown under iron deficiency and salinity","volume":"175","author":"N Boukari","year":"2023","journal-title":"Physiol Plant"},{"issue":"16","key":"pone.0335634.ref046","doi-asserted-by":"crossref","first-page":"5903","DOI":"10.1093\/jxb\/ers239","article-title":"Rosette iron deficiency transcript and microRNA profiling reveals links between copper and iron homeostasis in Arabidopsis thaliana","volume":"63","author":"BM Waters","year":"2012","journal-title":"J Exp Bot"},{"issue":"1","key":"pone.0335634.ref047","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1146\/annurev-arplant-070623-105324","article-title":"Metal Homeostasis in land plants: a perpetual balancing act beyond the fulfilment of metalloproteome cofactor demands","volume":"75","author":"U Kr\u00e4mer","year":"2024","journal-title":"Annu Rev Plant Biol"},{"key":"pone.0335634.ref048","first-page":"5","article-title":"Metal transport systems in plants","volume":"46","author":"S Huang","year":"2025","journal-title":"Annu Rev Plant Biol"},{"issue":"6","key":"pone.0335634.ref049","doi-asserted-by":"crossref","DOI":"10.1111\/ppl.14645","article-title":"TaCCS1-B expression modulates copper, enzymatic antioxidants and polyphenols contents and provides abiotic stress tolerance in transgenic Arabidopsis","volume":"176","author":"S Tyagi","year":"2024","journal-title":"Physiol Plant"},{"issue":"6","key":"pone.0335634.ref050","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1007\/s11103-019-00840-y","article-title":"The mitochondrial copper chaperone COX19 influences copper and iron homeostasis in arabidopsis","volume":"99","author":"L Garcia","year":"2019","journal-title":"Plant Mol Biol"},{"key":"pone.0335634.ref051","doi-asserted-by":"crossref","first-page":"529473","DOI":"10.3389\/fpls.2020.01106","article-title":"Deregulated high affinity copper transport alters iron homeostasis in Arabidopsis","volume":"11","author":"A Perea-Garc\u00eda","year":"2020","journal-title":"Front Plant Sci"}],"container-title":["PLOS One"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0335634","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T17:24:42Z","timestamp":1761931482000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0335634"}},"subtitle":[],"editor":[{"given":"Gokhan","family":"Hacisalihoglu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,10,31]]},"references-count":51,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2025,10,31]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0335634","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,31]]}}}