{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T05:26:56Z","timestamp":1778218016560,"version":"3.51.4"},"reference-count":17,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T00:00:00Z","timestamp":1764633600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Scientific Research Project of the Wuxi Municipal Health Commission","award":["Z202408"],"award-info":[{"award-number":["Z202408"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>Premature rupture of membranes (PROM) is a major contributor to preterm birth and neonatal morbidity. Current diagnostics are predominantly reactive and detect rupture only after it occurs. In a clinical cohort of 60 pregnant women (20 with PROM, 40 gestational controls at 31\u201336 weeks), we developed and validated a colorimetric assay for N-acetyl-D-galactosamine (GalNAc) in vaginal secretions. GalNAc levels were significantly lower in the PROM group than in controls, and the assay exhibited linearity, precision, stability, and resistance to interference. Receiver-operating characteristic analysis yielded an AUC of 0.821 (95% CI 0.80\u20130.97). These data support reduced vaginal GalNAc as a potential non-invasive risk indicator of PROM. While longitudinal validation is required, this approach may enable early risk stratification to guide preventive interventions.<\/jats:p>","DOI":"10.1371\/journal.pone.0335684","type":"journal-article","created":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T18:30:21Z","timestamp":1764700221000},"page":"e0335684","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["GalNAc in vaginal secretions: a potential non-invasive risk indicator associated with the risk of premature rupture of 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