{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T16:10:36Z","timestamp":1787069436391,"version":"build-2736575974"},"reference-count":60,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T00:00:00Z","timestamp":1761868800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002349","name":"Academy of Scientific Research and Technology","doi-asserted-by":"publisher","award":["ASRT5105"],"award-info":[{"award-number":["ASRT5105"]}],"id":[{"id":"10.13039\/501100002349","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004686","name":"Deanship of Scientific Research, King Faisal University","doi-asserted-by":"publisher","award":["KFU252322"],"award-info":[{"award-number":["KFU252322"]}],"id":[{"id":"10.13039\/501100004686","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>This study evaluated the impact of pre-freezing semen dilution rate and dimethyl acetamide (DMA) concentration on the post-thaw motility and fertility of cryopreserved rooster sperm. Rooster ejaculates were diluted with a standard EK extender to achieve low (LSC; 1\u2009\u00d7\u200910\u2079 sperm\/mL) and high (HSC; 2\u2009\u00d7\u200910\u2079 sperm\/mL) sperm concentrations. Each dilution group was further treated with three DMA concentrations (3%, 6%, or 9%) before cryopreservation. Post-thaw sperm motility traits were obtained by computer-assisted sperm analysis (CASA), and fertility features were evaluated through artificial insemination in hens. The current results showed that HSC significantly improved total motility (TM), curvilinear velocity (VCL), amplitude of lateral head displacement (ALH), and beat cross frequency (BCF), but reduced linearity (LIN) and straightness (STR) compared to LSC. DMA concentration had a quadratic effect on motility, with 6% yielding the highest progressive motility (PM), straight line velocity (VSL), and BCF. Fertility outcomes revealed that HSC resulted in higher fertilization rates, while neither DMA concentrations nor their interaction with dilution rates exerted significant effects on fertility traits. VCL, ALH, and BCF showed positive correlations with pipping-chicks rates, whereas STR, LIN, and WOB displayed negative correlations. These findings underscore the critical interplay between dilution rate and cryoprotectant concentration and provide practical guidance for developing more reliable cryopreservation protocols that can be applied under field conditions to enhance fertility management in poultry production.<\/jats:p>","DOI":"10.1371\/journal.pone.0335748","type":"journal-article","created":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T17:22:43Z","timestamp":1761931363000},"page":"e0335748","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effect of semen dilution rate and dimethyl acetamide levels on post-thaw motility and fertility parameters of rooster sperm"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0903-1036","authenticated-orcid":true,"given":"Mohamed","family":"Shawky","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-9161-5897","authenticated-orcid":true,"given":"Ahmed F.","family":"Fawy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6115-8408","authenticated-orcid":true,"given":"Ahmed M.","family":"Elomda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed A.","family":"Elmenawey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abd El-Rahman M.","family":"Atta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed O.","family":"Abbas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2006-4290","authenticated-orcid":true,"given":"Gamal M. K.","family":"Mehaisen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,10,31]]},"reference":[{"issue":"1","key":"pone.0335748.ref001","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1186\/s40104-022-00768-2","article-title":"Poultry genetic heritage cryopreservation and reconstruction: advancement and future challenges","volume":"13","author":"Y Sun","year":"2022","journal-title":"J Anim Sci Biotechnol"},{"key":"pone.0335748.ref002","doi-asserted-by":"crossref","first-page":"106904","DOI":"10.1016\/j.anireprosci.2021.106904","article-title":"Advances in sperm cryopreservation in farm animals: Cattle, horse, pig and sheep","volume":"246","author":"I Y\u00e1nez-Ortiz","year":"2022","journal-title":"Anim Reprod Sci"},{"issue":"2","key":"pone.0335748.ref003","first-page":"59","article-title":"PERSPECTIVE: Rooster Spermatozoa Cryopreservation and Quality Assessment","volume":"42","author":"A Svoradova","year":"2021","journal-title":"Cryo Letters"},{"issue":"11","key":"pone.0335748.ref004","doi-asserted-by":"crossref","first-page":"104190","DOI":"10.1016\/j.psj.2024.104190","article-title":"The characteristics of frozen-thawed rooster sperm using various intracellular cryoprotectants","volume":"103","author":"AM Elomda","year":"2024","journal-title":"Poult Sci"},{"issue":"10","key":"pone.0335748.ref005","doi-asserted-by":"crossref","first-page":"1603","DOI":"10.3390\/ani13101603","article-title":"The In Vitro Evaluation of Rooster Semen Pellets Frozen with Dimethylacetamide","volume":"13","author":"SK Hamad","year":"2023","journal-title":"Animals (Basel)"},{"issue":"20","key":"pone.0335748.ref006","doi-asserted-by":"crossref","first-page":"2739","DOI":"10.3390\/ani12202739","article-title":"Effect of Dimethylacetamide Concentration on Motility, Quality, Antioxidant Biomarkers, Anti-Freeze Gene Expression, and Fertilizing Ability of Frozen\/Thawed Rooster Sperm","volume":"12","author":"GMK Mehaisen","year":"2022","journal-title":"Animals (Basel)"},{"issue":"6","key":"pone.0335748.ref007","doi-asserted-by":"crossref","first-page":"101840","DOI":"10.1016\/j.psj.2022.101840","article-title":"Effect of glycerol concentration, glycerol removal method, and straw type on the quality and fertility of frozen chicken semen","volume":"101","author":"Y Zong","year":"2022","journal-title":"Poult Sci"},{"key":"pone.0335748.ref008","doi-asserted-by":"crossref","first-page":"106921","DOI":"10.1016\/j.anireprosci.2021.106921","article-title":"Advances in storage of poultry semen","volume":"246","author":"A Partyka","year":"2022","journal-title":"Anim Reprod Sci"},{"issue":"4","key":"pone.0335748.ref009","doi-asserted-by":"crossref","first-page":"297","DOI":"10.2141\/jpsa.009111","article-title":"A Method for Cryopreserving Semen from Yakido Roosters Using N-Methylacetamide as a Cryoprotective Agent","volume":"47","author":"K Sasaki","year":"2010","journal-title":"J Poult Sci"},{"issue":"2","key":"pone.0335748.ref010","doi-asserted-by":"crossref","first-page":"52","DOI":"10.3184\/175815511X13069413108523","article-title":"Freezing Avian Semen","volume":"4","author":"E Blesbois","year":"2011","journal-title":"Avian Biology Research"},{"issue":"2","key":"pone.0335748.ref011","doi-asserted-by":"crossref","DOI":"10.23880\/NNOA-16000139","article-title":"Efficiencies of the Cryoprotectants N-Methylacetamide, N-Methylformamide and Dimethyl Sulfoxide, and the Cell Protectants Trehalose and Hydroxyethyl Starch in Cryopreservation of Swine Sperm","volume":"3","author":"T Osuga","year":"2018","journal-title":"NNOA"},{"key":"pone.0335748.ref012","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1146\/annurev-animal-022513-114214","article-title":"From germ cell preservation to regenerative medicine: an exciting research career in biotechnology","volume":"2","author":"I Wilmut","year":"2014","journal-title":"Annu Rev Anim Biosci"},{"issue":"10","key":"pone.0335748.ref013","doi-asserted-by":"crossref","first-page":"2184","DOI":"10.3382\/ps.2008-00402","article-title":"Evaluation of glycerol removal techniques, cryoprotectants, and insemination methods for cryopreserving rooster sperm with implications of regeneration of breed or line or both","volume":"88","author":"PH Purdy","year":"2009","journal-title":"Poult Sci"},{"key":"pone.0335748.ref014","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/j.anireprosci.2010.10.010","article-title":"Cryoprotectant and freezing-process alter the ability of chicken sperm to acrosome react","volume":"122","author":"E Moc\u00e9","year":"2010","journal-title":"Anim Reprod Sci"},{"issue":"3","key":"pone.0335748.ref015","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1530\/rep.1.00454","article-title":"Membrane fluidity and the ability of domestic bird spermatozoa to survive cryopreservation","volume":"129","author":"E Blesbois","year":"2005","journal-title":"Reproduction"},{"issue":"6","key":"pone.0335748.ref016","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1016\/j.theriogenology.2010.04.032","article-title":"Evaluation of fresh and frozen-thawed fowl semen by flow cytometry","volume":"74","author":"A Partyka","year":"2010","journal-title":"Theriogenology"},{"key":"pone.0335748.ref017","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/j.anireprosci.2010.12.005","article-title":"Comparative cryopreservation of avian spermatozoa: benefits of non-permeating osmoprotectants and ATP on turkey and crane sperm cryosurvival","volume":"123","author":"JM Blanco","year":"2011","journal-title":"Anim Reprod Sci"},{"issue":"2","key":"pone.0335748.ref018","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.theriogenology.2014.04.007","article-title":"Pellet cryopreservation for chicken semen: effects of sperm working concentration, cryoprotectant concentration, and equilibration time during in vitro processing","volume":"82","author":"L Zaniboni","year":"2014","journal-title":"Theriogenology"},{"issue":"1","key":"pone.0335748.ref019","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1111\/rda.12464","article-title":"Effect of the interaction between cryoprotectant concentration and cryopreservation method on frozen\/thawed chicken sperm variables","volume":"50","author":"FMK Abouelezz","year":"2015","journal-title":"Reprod Domest Anim"},{"key":"pone.0335748.ref020","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.anireprosci.2017.07.021","article-title":"Fertility disturbances of dimethylacetamide and glycerol in rooster sperm diluents: Discrimination among effects produced pre and post freezing-thawing process","volume":"184","author":"FMK Abouelezz","year":"2017","journal-title":"Anim Reprod Sci"},{"key":"pone.0335748.ref021","doi-asserted-by":"crossref","first-page":"106238","DOI":"10.1016\/j.anireprosci.2019.106238","article-title":"Cryoprotective effect of melatonin supplementation on post-thawed rooster sperm quality","volume":"212","author":"GMK Mehaisen","year":"2020","journal-title":"Anim Reprod Sci"},{"issue":"8","key":"pone.0335748.ref022","doi-asserted-by":"crossref","first-page":"101091","DOI":"10.1016\/j.psj.2021.101091","article-title":"New semen freezing method for chicken and drake using dimethylacetamide as the cryoprotectant","volume":"100","author":"M Tang","year":"2021","journal-title":"Poult Sci"},{"issue":"2","key":"pone.0335748.ref023","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1093\/ps\/81.2.239","article-title":"Fertility of broiler breeders following categorization by the OptiBreed sperm quality index when hens are inseminated with a constant number of sperm","volume":"81","author":"HM Parker","year":"2002","journal-title":"Poult Sci"},{"issue":"3","key":"pone.0335748.ref024","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1093\/japr\/11.3.250","article-title":"Selection of Young Broiler Breeders for Semen Quality Improves Hatchability in an Industry Field Trial","volume":"11","author":"HM Parker","year":"2002","journal-title":"Journal of Applied Poultry Research"},{"issue":"1","key":"pone.0335748.ref025","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1093\/ps\/85.1.106","article-title":"The immediate impact of semen diluent and rate of dilution on the sperm quality index, ATP utilization, gas exchange, and ionic balance of broiler breeder sperm","volume":"85","author":"HM Parker","year":"2006","journal-title":"Poult Sci"},{"issue":"6","key":"pone.0335748.ref026","doi-asserted-by":"crossref","first-page":"907","DOI":"10.3390\/ani14060907","article-title":"Effect of Different Dilution Methods and Ratios of Ram Semen on Sperm Parameters after Cryopreservation","volume":"14","author":"L Zhang","year":"2024","journal-title":"Animals (Basel)"},{"key":"pone.0335748.ref027","doi-asserted-by":"crossref","first-page":"760","DOI":"10.4081\/ijas.2009.s2.760","article-title":"Effect of different sperm concentrations on the post-thaw viability and motility of turkey spermatozoa cryopreserved by the pellet method","volume":"8","author":"N Iaffaldano","year":"2009","journal-title":"Italian Journal of Animal Science"},{"issue":"5","key":"pone.0335748.ref028","doi-asserted-by":"crossref","first-page":"794","DOI":"10.1016\/j.theriogenology.2011.04.012","article-title":"Cryopreservation of turkey semen by the pellet method: effects of variables such as the extender, cryoprotectant concentration, cooling time and warming temperature on sperm quality determined through principal components analysis","volume":"76","author":"N Iaffaldano","year":"2011","journal-title":"Theriogenology"},{"issue":"1","key":"pone.0335748.ref029","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.theriogenology.2004.03.020","article-title":"Role of seminal plasma in damage to turkey spermatozoa during in vitro storage","volume":"63","author":"V Douard","year":"2005","journal-title":"Theriogenology"},{"issue":"2","key":"pone.0335748.ref030","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1017\/S0043933907001419","article-title":"Current status in avian semen cryopreservation","volume":"63","author":"E Blesbois","year":"2007","journal-title":"World\u2019s Poultry Science Journal"},{"key":"pone.0335748.ref031","doi-asserted-by":"crossref","first-page":"175","DOI":"10.5772\/54918","article-title":"Artificial insemination in poultry","volume-title":"Success in artificial insemination - quality of semen and diagnostics employed","author":"MR Bakst","year":"2013"},{"issue":"1","key":"pone.0335748.ref032","doi-asserted-by":"crossref","first-page":"101","DOI":"10.3382\/japr.2007-00048","article-title":"Effect of Semen Extenders and Storage Time on Sperm Morphology of Four Chicken Breeds","volume":"17","author":"A Siudzi\u0144ska","year":"2008","journal-title":"Journal of Applied Poultry Research"},{"issue":"1","key":"pone.0335748.ref033","doi-asserted-by":"crossref","first-page":"447","DOI":"10.3382\/ps\/pey360","article-title":"Chicken semen cryopreservation and use for the restoration of rare genetic resources","volume":"98","author":"A Th\u00e9lie","year":"2019","journal-title":"Poult Sci"},{"key":"pone.0335748.ref034","doi-asserted-by":"crossref","first-page":"106845","DOI":"10.1016\/j.anireprosci.2021.106845","article-title":"Assessing different liquid-storage temperatures for rooster spermatozoa","volume":"233","author":"MH Blank","year":"2021","journal-title":"Anim Reprod Sci"},{"issue":"11","key":"pone.0335748.ref035","doi-asserted-by":"crossref","first-page":"1359","DOI":"10.1292\/jvms.66.1359","article-title":"Effects of final dilution rate, sperm concentration and times for cooling and glycerol equilibration on post-thaw characteristics of canine spermatozoa","volume":"66","author":"T Okano","year":"2004","journal-title":"J Vet Med Sci"},{"issue":"7","key":"pone.0335748.ref036","doi-asserted-by":"crossref","first-page":"411","DOI":"10.3923\/ajava.2016.411.416","article-title":"Effect of Semen Diluents, Dilution Rates and Storage Periods on Live and Abnormal Spermatozoa of Pearl Guinea Fowls","volume":"11","author":"GH Hudson","year":"2016","journal-title":"Asian J of Animal and Veterinary Advances"},{"issue":"5","key":"pone.0335748.ref037","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1080\/00071668.2017.1354355","article-title":"Spermatozoa DNA and plasma membrane integrity after pellet optimized processing for cryopreservation in meat type chicken breeders","volume":"58","author":"TM Gliozzi","year":"2017","journal-title":"Br Poult Sci"},{"issue":"8","key":"pone.0335748.ref038","doi-asserted-by":"crossref","first-page":"1497","DOI":"10.1016\/j.theriogenology.2011.11.006","article-title":"Effect of cryopreservation on sperm parameters, lipid peroxidation and antioxidant enzymes activity in fowl semen","volume":"77","author":"A Partyka","year":"2012","journal-title":"Theriogenology"},{"issue":"11","key":"pone.0335748.ref039","doi-asserted-by":"crossref","first-page":"6139","DOI":"10.1016\/j.psj.2020.06.088","article-title":"Comparative studies of semen quality traits and sperm kinematic parameters in relation to fertility rate between 2 genetic groups of breed lines","volume":"99","author":"HH Tesfay","year":"2020","journal-title":"Poult Sci"},{"issue":"9","key":"pone.0335748.ref040","doi-asserted-by":"crossref","DOI":"10.1242\/jeb.243715","article-title":"The impact of diet quality on the velocity, morphology and normality of sperm in the zebra finch Taeniopygia guttata","volume":"225","author":"CS McDiarmid","year":"2022","journal-title":"J Exp Biol"},{"issue":"9","key":"pone.0335748.ref041","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.3390\/ani12091104","article-title":"Mass Sperm Motility Is Correlated to Sperm Motility as Measured by Computer-Aided Sperm Analysis (CASA) Technology in Farmed Ostriches","volume":"12","author":"PT Muvhali","year":"2022","journal-title":"Animals (Basel)"},{"key":"pone.0335748.ref042","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.anireprosci.2012.07.007","article-title":"Lipid peroxidation and antioxidant enzymes activity in avian semen","volume":"134","author":"A Partyka","year":"2012","journal-title":"Anim Reprod Sci"},{"issue":"1","key":"pone.0335748.ref043","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0209910","article-title":"Seminal plasma amino acid profile in different breeds of chicken: Role of seminal plasma on sperm cryoresistance","volume":"14","author":"J Santiago-Moreno","year":"2019","journal-title":"PLoS One"},{"key":"pone.0335748.ref044","doi-asserted-by":"crossref","first-page":"106304","DOI":"10.1016\/j.anireprosci.2020.106304","article-title":"\u201cSemen dilution effect\u201d on sperm variables and conception rate in buffalo","volume":"214","author":"S Patil","year":"2020","journal-title":"Anim Reprod Sci"},{"issue":"4","key":"pone.0335748.ref045","article-title":"Effect of Dilution and Sperm Concentration on Post Thaw Semen Quality in Barbari Buck","volume":"8","author":"M Anand","year":"2018","journal-title":"JAR"},{"issue":"4","key":"pone.0335748.ref046","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1016\/j.theriogenology.2014.11.012","article-title":"The \u201cdilution effect\u201d in stallion sperm","volume":"83","author":"SS Hayden","year":"2015","journal-title":"Theriogenology"},{"issue":"16","key":"pone.0335748.ref047","doi-asserted-by":"crossref","first-page":"5664","DOI":"10.3390\/ijms21165664","article-title":"Functional Aspects of Seminal Plasma in Bird Reproduction","volume":"21","author":"J Santiago-Moreno","year":"2020","journal-title":"Int J Mol Sci"},{"key":"pone.0335748.ref048","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.anireprosci.2016.09.004","article-title":"Cryopreservation of Indian red jungle fowl (Gallus gallus murghi) semen","volume":"174","author":"BA Rakha","year":"2016","journal-title":"Anim Reprod Sci"},{"key":"pone.0335748.ref049","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/S0378-4320(00)00160-3","article-title":"Storage of poultry semen","volume":"62","author":"AM Donoghuea","year":"2000","journal-title":"Anim Reprod Sci"},{"issue":"11","key":"pone.0335748.ref050","doi-asserted-by":"crossref","first-page":"6071","DOI":"10.3382\/ps\/pez303","article-title":"Effect of dimethylacetamide and N-methylacetamide on the quality and fertility of frozen\/thawed chicken semen","volume":"98","author":"F Mosca","year":"2019","journal-title":"Poult Sci"},{"key":"pone.0335748.ref051","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.cryobiol.2022.04.001","article-title":"Concentration dependent effect of dimethylacetamide and N-methylacetamide on the quality and fertility of cryopreserved chicken semen","volume":"106","author":"L Zaniboni","year":"2022","journal-title":"Cryobiology"},{"issue":"1","key":"pone.0335748.ref052","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0209910","article-title":"Seminal plasma amino acid profile in different breeds of chicken: Role of seminal plasma on sperm cryoresistance","volume":"14","author":"J Santiago-Moreno","year":"2019","journal-title":"PLoS One"},{"key":"pone.0335748.ref053","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.theriogenology.2020.03.023","article-title":"Effect of dimethylformamide on sperm quality and fertilizing ability of Indian red jungle fowl (Gallus gallus murghi)","volume":"149","author":"BA Rakha","year":"2020","journal-title":"Theriogenology"},{"issue":"11","key":"pone.0335748.ref054","doi-asserted-by":"crossref","first-page":"104190","DOI":"10.1016\/j.psj.2024.104190","article-title":"The characteristics of frozen-thawed rooster sperm using various intracellular cryoprotectants","volume":"103","author":"AM Elomda","year":"2024","journal-title":"Poult Sci"},{"issue":"2","key":"pone.0335748.ref055","doi-asserted-by":"crossref","first-page":"445","DOI":"10.3390\/agriculture13020445","article-title":"Chicken Sperm Cryopreservation: Review of Techniques, Freezing Damage, and Freezability Mechanisms","volume":"13","author":"Y Zong","year":"2023","journal-title":"Agriculture"},{"key":"pone.0335748.ref056","doi-asserted-by":"crossref","first-page":"8703","DOI":"10.1038\/ncomms9703","article-title":"Two-dimensional slither swimming of sperm within a micrometre of a surface","volume":"6","author":"R Nosrati","year":"2015","journal-title":"Nat Commun"},{"key":"pone.0335748.ref057","doi-asserted-by":"crossref","first-page":"1","DOI":"10.15517\/am.v32i1.41928","article-title":"Frecuencia de fotogramas \u00f3ptima para evaluar la cin\u00e9tica esperm\u00e1tica de verracos con un sistema CASA-Mot","author":"V Barquero","year":"2021","journal-title":"Agron Mesoam"},{"issue":"8","key":"pone.0335748.ref058","doi-asserted-by":"crossref","DOI":"10.48077\/scihor.25(8).2022.67-74","article-title":"Interrelation of Kinetic Parameters of Sperm of Servicing Bulls of the Holstein Breed with Its Fertilising Ability","volume":"25","author":"L Piddubna","year":"2022","journal-title":"Scientific Horizons"},{"issue":"3","key":"pone.0335748.ref059","doi-asserted-by":"crossref","first-page":"189","DOI":"10.22358\/jafs\/127691\/2020","article-title":"The application of computer-assisted semen analysis (CASA) technology to optimise semen evaluation. A review","volume":"29","author":"A Valverde","year":"2020","journal-title":"J Anim Feed Sci"},{"key":"pone.0335748.ref060","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.theriogenology.2018.04.012","article-title":"Correlation between objective semen analysis and fertility in Japanese quail","volume":"115","author":"U Farooq","year":"2018","journal-title":"Theriogenology"}],"container-title":["PLOS One"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0335748","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T17:22:52Z","timestamp":1761931372000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0335748"}},"subtitle":[],"editor":[{"given":"Sameh","family":"Abdelnour","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,10,31]]},"references-count":60,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2025,10,31]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0335748","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,31]]}}}