{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T17:55:19Z","timestamp":1790618119649,"version":"4.1.0"},"reference-count":52,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T00:00:00Z","timestamp":1762387200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Ulcerative colitis (UC) is a chronic inflammatory disease of the colon, marked by symptoms such as mucosal ulceration, diarrhea, and abdominal pain. The dextran sodium sulfate (DSS)-induced murine model replicates clinical and histological features of UC and is widely used to explore potential treatments.\n                    <jats:italic>Lactobacillus reuteri<\/jats:italic>\n                    has emerged as a promising probiotic due to its immunomodulatory and anti-inflammatory properties. This study investigates the effects of\n                    <jats:italic>L. reuteri<\/jats:italic>\n                    supplementation on histopathology, inflammatory cytokines, intestinal barrier function, and short-chain fatty acid (SCFA) production in DSS-induced colitis. Male Balb\/C mice were divided into control, DSS-induced colitis, and DSS\u2009+\u2009\n                    <jats:italic>L. reuteri<\/jats:italic>\n                    treatment groups. The colitis model was established with 3% DSS in drinking water for seven days, and mice in the treatment group received 10\n                    <jats:sup>10<\/jats:sup>\n                    CFU of\n                    <jats:italic>L. reuteri<\/jats:italic>\n                    daily. Disease activity index (DAI), colon length, myeloperoxidase (MPO) levels, cytokine concentrations, tight junction protein expression, and SCFA production were measured to evaluate treatment effects. Histological analyses assessed inflammation, crypt damage, and ulceration. Mice treated with\n                    <jats:italic>L. reuteri<\/jats:italic>\n                    exhibited significant improvements across all evaluated parameters. Supplementation mitigated weight loss, reduced DAI, and restored colon length. MPO levels and pro-inflammatory cytokines (TNF-\u03b1, IL-1\u03b2, IL-6) were significantly reduced, while anti-inflammatory IL-10 levels were elevated. Histological scores showed decreased inflammation, crypt damage, and ulceration.\n                    <jats:italic>L. reuteri<\/jats:italic>\n                    enhanced tight junction protein expression, particularly ZO-1 and Claudin-1, and significantly increased SCFA production, improving gut barrier integrity and microbial function.\n                    <jats:italic>L. reuteri<\/jats:italic>\n                    supplementation effectively mitigates DSS-induced colitis by reducing inflammation, restoring intestinal barrier integrity, and enhancing microbial metabolism. These findings suggest\n                    <jats:italic>L. reuteri<\/jats:italic>\n                    as a promising therapeutic candidate for UC management.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0335942","type":"journal-article","created":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T18:39:18Z","timestamp":1762454358000},"page":"e0335942","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":4,"title":["The protective role of Lactobacillus reuteri in a DSS-induced ulcerative colitis mouse model"],"prefix":"10.1371","volume":"20","author":[{"given":"Mohsen","family":"Nazari","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rezvan","family":"Goodarzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atefeh","family":"Yazdan Panah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iraj","family":"Amiri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasser","family":"Bagheri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4038-9618","authenticated-orcid":true,"given":"Babak","family":"Asghari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,11,6]]},"reference":[{"issue":"1","key":"pone.0335942.ref001","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1007\/s00394-020-02252-x","article-title":"Alleviation effects of Bifidobacterium breve on DSS-induced colitis depends on intestinal tract barrier maintenance and gut microbiota modulation","volume":"60","author":"Y Chen","year":"2021","journal-title":"Eur J Nutr"},{"issue":"2","key":"pone.0335942.ref002","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.ijfoodmicro.2004.11.032","article-title":"Probiotic effects of Lactobacillus casei on DSS-induced ulcerative colitis in mice","volume":"103","author":"MV Her\u00edas","year":"2005","journal-title":"Int J Food Microbiol"},{"issue":"1","key":"pone.0335942.ref003","article-title":"Dextran sulfate sodium (DSS)\u2010induced colitis in mice","volume":"104","author":"B Chassaing","year":"2014","journal-title":"Curr Protocol Immunol"},{"issue":"6","key":"pone.0335942.ref004","doi-asserted-by":"crossref","first-page":"776","DOI":"10.3390\/foods11060776","article-title":"Protective Effects of Fermented Soybeans (Cheonggukjang) on Dextran Sodium Sulfate (DSS)-Induced Colitis in a Mouse Model","volume":"11","author":"H-J Lim","year":"2022","journal-title":"Foods"},{"issue":"2","key":"pone.0335942.ref005","first-page":"215","article-title":"Novel therapeutic strategy for obesity through the gut microbiota-brain axis: A review article","volume":"15","author":"R Kardan","year":"2024","journal-title":"Caspian J Intern Med"},{"issue":"10","key":"pone.0335942.ref006","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1038\/nrgastro.2012.152","article-title":"The gut microbiota in IBD","volume":"9","author":"C Manichanh","year":"2012","journal-title":"Nat Rev Gastroenterol Hepatol"},{"issue":"2","key":"pone.0335942.ref007","doi-asserted-by":"crossref","first-page":"145","DOI":"10.3390\/ani12020145","article-title":"Integrity of the Intestinal Barrier: The Involvement of Epithelial Cells and Microbiota-A Mutual Relationship","volume":"12","author":"M Giery\u0144ska","year":"2022","journal-title":"Animals (Basel)"},{"key":"pone.0335942.ref008","doi-asserted-by":"crossref","first-page":"718356","DOI":"10.3389\/fnut.2021.718356","article-title":"Inflammatory and Microbiota-Related Regulation of the Intestinal Epithelial Barrier","volume":"8","author":"G Barbara","year":"2021","journal-title":"Front Nutr"},{"issue":"4","key":"pone.0335942.ref009","doi-asserted-by":"crossref","first-page":"979","DOI":"10.3390\/ani11040979","article-title":"Bacteriocins from Lactic Acid Bacteria. 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