{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T13:56:18Z","timestamp":1784555778695,"version":"3.55.0"},"reference-count":22,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T00:00:00Z","timestamp":1764115200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"\n Background<\/jats:title>\n Early diagnosis and prompt treatment of malaria cases are a crucial component of curative and preventive interventions. There have been reports of healthcare workers overprescribing antimalarial agents against guidelines, but the barriers they face in adhering to the guidelines are not well studied. This study aimed to investigate barriers to adherence to guidelines in prescribing antimalarial drugs in public healthcare facilities in Arba Minch, South Ethiopia.<\/jats:p>\n <\/jats:sec>\n \n Method<\/jats:title>\n A cross-sectional descriptive exploratory qualitative method was employed. We included ten participants from public healthcare facilities, including health centres, a hospital, a city health office, and a zonal health bureau. A key informant interview technique was used to collect data. All interviews were audio-recorded, transcribed, and analyzed. Data analysis was performed using ATLAS.ti, version 7.5 software. The results were presented thematically and narrated to support the main themes.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n Public healthcare facilities primarily used blood smear microscopy to test all malaria-suspected patients. However, in cases of microscopy service interruptions or when confirming negative results, rapid diagnostic tests (RDTs) were employed in some facilities. Limited availability of microscopes and reagents, and electric power interruptions hindered reliable microscopy services. Drug stock-outs, patient expectations for antimalarial drugs, self-treatment, and delayed care-seeking are barriers to adherence to malaria treatment guidelines. The main reason for non-adherence to withholding antimalarial drugs after negative tests was greater trust in clinical findings over laboratory results. Confidence in experience contributed to trust in clinical judgment, while perceived inexperience and negligence, inconsistent RDT and microscopy results, and poor-quality control assessment results undermined trust in laboratories. Despite supporting the guidelines, study participants emphasized the need for flexibility to allow empirical treatment and highlighted the lack of training and mentoring for healthcare workers.<\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n To improve adherence to malaria treatment guidelines, it is essential to ensure consistent lab operations, enhance quality assurance, maintain effective communication between lab personnel and prescribers, and provide healthcare and patient education. Implementing training and mentoring programs and promoting evidence-based practices are also crucial.<\/jats:p>\n <\/jats:sec>","DOI":"10.1371\/journal.pone.0337326","type":"journal-article","created":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T18:29:53Z","timestamp":1764181793000},"page":"e0337326","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["Investigating barriers to adherence to antimalarial prescribing guidelines in public healthcare facilities in Arba Minch, South Ethiopia: A qualitative study"],"prefix":"10.1371","volume":"20","author":[{"given":"Abate Atimut","family":"Dereje","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dereje","family":"Geleta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2510-604X","authenticated-orcid":true,"given":"Tadesse","family":"Menjetta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abinet","family":"Takele","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susana","family":"Vaz Nery","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3531-0482","authenticated-orcid":true,"given":"Techalew","family":"Shimelis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,11,26]]},"reference":[{"key":"pone.0337326.ref001","author":"World Health Organization","year":"2023"},{"key":"pone.0337326.ref002","doi-asserted-by":"crossref","first-page":"8863002","DOI":"10.1155\/2021\/8863002","article-title":"Prevalence of malaria among adults in Ethiopia: a systematic review and meta-analysis","volume":"2021","author":"FA Kendie","year":"2021","journal-title":"J Trop Med"},{"issue":"9","key":"pone.0337326.ref003","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0009781","article-title":"Plasmodium vivax epidemiology in Ethiopia 2000-2020: a systematic review and meta-analysis","volume":"15","author":"T Ketema","year":"2021","journal-title":"PLoS Negl Trop Dis"},{"key":"pone.0337326.ref004","author":"Federal Ministry of Health","year":"2023"},{"key":"pone.0337326.ref005","author":"World Health Organization","year":"2023"},{"issue":"3","key":"pone.0337326.ref006","first-page":"270","article-title":"Malaria: prevention, diagnosis, and treatment","volume":"106","author":"SD Shahbodaghi","year":"2022","journal-title":"Am Fam Physician"},{"key":"pone.0337326.ref007","doi-asserted-by":"crossref","first-page":"1006659","DOI":"10.3389\/fmicb.2022.1006659","article-title":"Advances and challenges in automated malaria diagnosis using digital microscopy imaging with artificial intelligence tools: a review","volume":"13","author":"CR Maturana","year":"2022","journal-title":"Front Microbiol"},{"key":"pone.0337326.ref008","volume-title":"Pediatric hospital care: Ethiopia. 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