{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T18:40:31Z","timestamp":1764096031277,"version":"3.46.0"},"reference-count":46,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T00:00:00Z","timestamp":1764028800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science and Technology Project of Liaoning Province","award":["2023JH2\/101700111"],"award-info":[{"award-number":["2023JH2\/101700111"]}]},{"name":"Shenyang City Young and Middle-Aged Science and Technology Innovation Talents Cultivation Special Outstanding Young and Middle-Aged Project","award":["RC230653"],"award-info":[{"award-number":["RC230653"]}]},{"name":"Provincial Joint Fund General Program","award":["2023-MSLH-343"],"award-info":[{"award-number":["2023-MSLH-343"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Radiation-induced brain injury (RIBI) is a significant complication following radiotherapy for brain tumors, leading to neurocognitive deficits and other neurological impairments. This study aims to identify potential biomarkers and therapeutic targets for RIBI by utilizing advanced proteomic techniques to explore the molecular mechanisms underlying RIBI.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A rat model of RIBI was established and subjected to whole-brain irradiation (30 Gy). Tandem mass tagging (TMT)-based quantitative proteomics, combined with high-resolution mass spectrometry, was used to identify differentially expressed proteins (DEPs) in the brain tissues of irradiated rats. Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were conducted to identify the biological processes and pathways involved. Protein-protein interaction (PPI) networks were constructed to identify key hub proteins.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 35 DEPs were identified, including PHLDA3, APOE and CPE. GO enrichment analysis revealed that the DEPs were mainly involved in lipid transport, cell adhesion, and metabolic processes. KEGG analysis highlighted the enrichment of pathways related to metabolism, tight junctions, and PPAR signaling. APOE was identified as a key hub protein through PPI network analysis, indicating its potential role in RIBI pathophysiology. Immunohistochemistry further validated the increased expression of PHLDA3, APOE, and CPE in the brain tissue of irradiated rats.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study provides valuable insights into the molecular mechanisms of RIBI by identifying key proteins and their associated pathways. The findings suggest that these proteins, particularly APOE and PHLDA3, could serve as potential biomarkers and therapeutic targets for clinical intervention in RIBI. These results not only enhance our understanding of RIBI\u2019s molecular pathology but also open new avenues for the development of targeted therapies to mitigate radiation-induced neurotoxicity.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pone.0337608","type":"journal-article","created":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T18:28:57Z","timestamp":1764095337000},"page":"e0337608","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Quantitative proteomic analysis reveals key proteins involved in radiation-induced brain injury"],"prefix":"10.1371","volume":"20","author":[{"given":"Jing","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junshuang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuang","family":"Lv","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hengjiao","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Defu","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huiling","family":"Qu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5523-4168","authenticated-orcid":true,"given":"Ying","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Yan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2025,11,25]]},"reference":[{"issue":"1","key":"pone.0337608.ref001","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.hoc.2021.08.006","article-title":"Update on radiation therapy for central nervous system tumors","volume":"36","author":"R Rahman","year":"2022","journal-title":"Hematol Oncol Clin North Am"},{"issue":"4","key":"pone.0337608.ref002","doi-asserted-by":"crossref","first-page":"35","DOI":"10.21037\/cco.2017.06.02","article-title":"An update on radiation therapy for brain metastases","volume":"6","author":"T-C Lam","year":"2017","journal-title":"Chin Clin Oncol"},{"issue":"8","key":"pone.0337608.ref003","first-page":"3275","article-title":"Advances in the study of the molecular biological mechanisms of radiation-induced brain injury","volume":"13","author":"C Gan","year":"2023","journal-title":"Am J Cancer Res"},{"issue":"1","key":"pone.0337608.ref004","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1667\/RADE-20-00147.1","article-title":"Brain Damage and patterns of neurovascular disorder after ionizing irradiation. 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