{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T17:40:22Z","timestamp":1793036422618,"version":"4.5.0"},"reference-count":43,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T00:00:00Z","timestamp":1779148800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["INV-070227"],"award-info":[{"award-number":["INV-070227"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["INV-025569"],"award-info":[{"award-number":["INV-025569"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["OPP1198779, OPP1213975"],"award-info":[{"award-number":["OPP1198779, OPP1213975"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Combining larviciding with insecticide treated nets (ITNs) can reduce malaria transmission. However, most modelling analyses use generalized scenarios rather than incorporating local epidemiological and ecological contexts. In Tanzania and other countries, larviciding is increasingly being prioritized in national strategies, with growing advocacy for its broader implementation, to achieving sustained malaria reduction. District-specific modelling is therefore essential to capture variation in transmission ecology, seasonality, and varying coverage levels, providing evidence that is both rigorous and actionable for malaria control programs. The Vector Control Optimization Model (VCOM) was adapted and extended to incorporate local seasonality, simulating the impact of larviciding across a range of coverage levels combined with ITNs. The model was parameterized using district-level field-data on mosquito mortality collected before (2016\u20132017) and after (2019\u20132021) larviciding implementation. Mosquito mortality rates were estimated using Bayesian inference. Outcomes were evaluated specifically for\n                    <jats:italic>Anopheles gambiae<\/jats:italic>\n                    s.l. including annual entomological inoculation rates (EIR) and mosquito density. Sensitivity analysis explored the influence of key parameters driving transmission in this scenario study. The immature mosquito mortality rate due to larviciding was 61% based on field data. VCOM simulation showed that, at 80%, ITNs coverage, larviciding substantially reduced mosquito densities and EIR. Specifically, combining ITNs at 80% and larviciding coverage\u2009\u2265\u200960% lowered EIR below 1 ib\/p\/year, the threshold required to interrupt malaria transmission. Sensitivity analyses highlighted the high impact of targeting immature mosquitoes, suggesting larviciding can effectively complement ITNs to control vectors, including invasive species like\n                    <jats:italic>An. stephensi,<\/jats:italic>\n                    regardless of feeding preference, resting, and biting behaviors, which hinder the effectiveness of most vector control tools. This study provides local evidence that larviciding is an effective complement to ITNs for interrupting malaria transmission. Implementation should leverage innovative approaches, such as drones for precise mapping and targeted application of biological larvicides, to maximize coverage, and scalability for district-level malaria control and elimination.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0337662","type":"journal-article","created":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T17:32:12Z","timestamp":1779211932000},"page":"e0337662","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Modelling the impact of larviciding as a supplementary malaria vector control intervention in rural south-eastern Tanzania: A district-level simulation study"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-5769-855X","authenticated-orcid":true,"given":"GloriaSalome","family":"Shirima","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1598-962X","authenticated-orcid":true,"given":"Emma L.","family":"Fairbanks","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tegemeo","family":"Gavana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerald","family":"Kiwelu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ismail","family":"Nambunga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeromin P.","family":"Mlacha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silas","family":"Mirau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prosper","family":"Chaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nakul","family":"Chitnis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samson","family":"Kiware","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,5,19]]},"reference":[{"issue":"8","key":"pone.0337662.ref001","first-page":"722","article-title":"Sir Ronald Ross and the evolution of malaria research","volume":"49","author":"M Yoeli","year":"1973","journal-title":"Bull N Y Acad Med"},{"key":"pone.0337662.ref002","unstructured":"Organization WH. 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