{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T13:29:59Z","timestamp":1773235799813,"version":"3.50.1"},"reference-count":66,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T00:00:00Z","timestamp":1769731200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007225","name":"Ministry of Science and Technology","doi-asserted-by":"publisher","award":["No. 20230030-20-21"],"award-info":[{"award-number":["No. 20230030-20-21"]}],"id":[{"id":"10.13039\/100007225","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>Mitigating enteric methane in the humid tropics, particularly in the Colombian Amazonian foothills, remains challenging due to limited field-based data under real grazing conditions. This study evaluated the performance of a laser methane detector (LMD) as a non-invasive alternative to traditional techniques, providing the first field-based validation of this approach in Amazonian grazing systems. Two contrasting production systems were compared: a silvopastoral system (SPS) with trees and shrubs, and a grass monoculture (traditional pasture, TP). A crossover design (two groups of five cows) was implemented across four periods. The LMD enabled repeated, activity measurements without disrupting natural behavior, capturing emissions during grazing, ruminating, resting, and milking. Daily CH\u2084 emissions were significantly lower in SPS than TP (233\u2009\u00b1\u20096.95 vs. 277\u2009\u00b1\u20098.87\u2009g CH\u2084 animal\u2009\u207b\u2009\u00b9 day\u2009\u207b\u2009\u00b9; p\u2009&lt;\u20090.0001). Methane intensity also decreased in SPS when expressed per kg milk (15.5 vs. 20.7\u2009g CH\u2084 kg\u2009\u207b\u2009\u00b9), energy-corrected milk (16.0 vs. 21.2\u2009g CH\u2084 kg\u2009\u207b\u2009\u00b9), and dry matter intake (18.9 vs. 26.7\u2009g CH\u2084 kg DMI\u2009\u207b\u2009\u00b9; all p\u2009&lt;\u20090.0001). Classification was based on animal activity rather than diet, allowing detailed behavioral associations with CH\u2084 release dynamics. While the LMD requires strict environmental protocols and does not capture continuous 24-h data, its portability and non-invasive nature make it a practical, scalable tool for tropical field conditions. These results provide novel evidence supporting SPS as a mitigation strategy, strengthen GHG inventories in tropical livestock systems, and offer guidance for policymakers promoting sustainable production systems.<\/jats:p>","DOI":"10.1371\/journal.pone.0337719","type":"journal-article","created":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T18:33:27Z","timestamp":1769798007000},"page":"e0337719","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Estimation of enteric methane emissions in dairy cows under grazing a silvopastoral system and a grass monoculture in the Colombian Amazonian foothills"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2766-2939","authenticated-orcid":true,"given":"Juan Pablo","family":"Narv\u00e1ez-Herrera","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joaqu\u00edn","family":"Angulo-Arizala","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wilson Andr\u00e9s","family":"Barrag\u00e1n-Hern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3377-8399","authenticated-orcid":true,"given":"Liliana","family":"Mahecha-Ledesma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2026,1,30]]},"reference":[{"issue":"11","key":"pone.0337719.ref001","doi-asserted-by":"crossref","first-page":"1269","DOI":"10.3390\/atmos15111269","article-title":"Combining the Eddy Covariance Method and Dry Matter Intake Measurements for Enteric Methane Emission Estimation from Grazing Dairy 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