{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T01:45:45Z","timestamp":1790991945264,"version":"4.1.0"},"reference-count":92,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T00:00:00Z","timestamp":1768262400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Green-synthesized nanoparticles (NPs) offer unique properties over traditional approaches, with applications in coatings, catalysts, packaging, environmental cleaning, and medicine. This research involves the synthesis of Zinc oxide nanoparticles (ZnO NPs) utilizing aqueous leaf extract of\n                    <jats:italic>Typha domingensis<\/jats:italic>\n                    (locally known as Hogla pata) as a reducing agent. The biosynthesized NPs were then characterized using ultraviolet-visible spectroscopy (UV-Vis), Fourier transform infrared (FTIR) spectroscopy, field emission scanning electron microscopy (FESEM), and energy-dispersive X-ray (EDX) spectroscopy. The XRD result revealed that the synthesized NPs were enriched with (101) facets, which were similar to the standard JCPDS card no 00-036-1451. The size of the ZnO NPs was determined from XRD data utilizing the Debye-Scherrer equation and found to be around 26.74\u2009nm. Moreover, the SEM analysis showed that the shape of the NPs was agglomerated spherical. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical showed dose-dependent antioxidant activity against synthesized ZnO NPs, with an average IC\n                    <jats:sub>50<\/jats:sub>\n                    of 32.25 \u00b5g\/mL. The antibacterial efficacy of green-synthesized ZnO NPs was assessed against fungal and bacterial pathogens in antifungal experiments.\n                    <jats:italic>Colletotrichum sp.<\/jats:italic>\n                    showed the highest sensitivity to ZnO NPs, with a 20\u2009\u00b1\u20090.80\u2009mm zone of inhibition (ZOI) and 22.22% inhibition, whereas\n                    <jats:italic>Fusarium sp.<\/jats:italic>\n                    had a 17\u2009\u00b1\u20090.68\u2009mm ZOI and 18.89% inhibition at a dosage of 2\u2009mg\/mL. ZnO NPs also exhibited antibacterial activity against two gram-negative bacteria,\n                    <jats:italic>Coliform sp.<\/jats:italic>\n                    and\n                    <jats:italic>Salmonella sp.<\/jats:italic>\n                    , both demonstrating a 12\u2009mm ZOI and 13.33% inhibition at 5.0\u2009mg\/mL. These results demonstrate that ZnO NPs biosynthesized with\n                    <jats:italic>Typha domingensis<\/jats:italic>\n                    leaf extract exhibit versatile antimicrobial and antioxidant properties, offering a safe and sustainable alternative to synthetic compounds.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0337942","type":"journal-article","created":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T18:37:17Z","timestamp":1768329437000},"page":"e0337942","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["Investigating the antioxidant, antibacterial, and antifungal properties of typha domingensis leaves assisted synthesized zinc oxide nanoparticles"],"prefix":"10.1371","volume":"21","author":[{"given":"Md. Suzon","family":"Ahmed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Ariful Islam","family":"Setu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atasi","family":"Goswami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mst. Sabiha","family":"Sultana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Palash Kumar","family":"Dhar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Rezaul","family":"Haque","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9071-9259","authenticated-orcid":true,"given":"Sagar Kumar","family":"Dutta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,1,13]]},"reference":[{"issue":"2","key":"pone.0337942.ref001","doi-asserted-by":"crossref","first-page":"115862","DOI":"10.1016\/j.jece.2025.115862","article-title":"Eco-friendly green synthesis and characterization of zirconium oxide nanoparticles using Ulva lactuca and their medical and environmental potential","volume":"13","author":"C Sudhakar","year":"2025","journal-title":"J Environ Chem Eng"},{"key":"pone.0337942.ref002","doi-asserted-by":"crossref","first-page":"122220","DOI":"10.1016\/j.ica.2024.122220","article-title":"Green synthesis of silver nanoparticles using Senna alexandrina leaf extracts and their antibacterial, larvicidal, nanotoxicity and photocatalytic activity","volume":"571","author":"V Sangameshwaran","year":"2024","journal-title":"Inorganica Chim Acta"},{"key":"pone.0337942.ref003","doi-asserted-by":"crossref","first-page":"142973","DOI":"10.1016\/j.molstruc.2025.142973","article-title":"Catalytic degradation of methyl orange and biomedical efficacy of sustainable silver nanoparticles from Boerhaavia diffusa leaf extract","volume":"1344","author":"C Sudhakar","year":"2025","journal-title":"J Mol Struct"},{"issue":"1","key":"pone.0337942.ref004","doi-asserted-by":"crossref","first-page":"112","DOI":"10.3390\/molecules25010112","article-title":"The history of nanoscience and nanotechnology: From chemical-physical applications to nanomedicine","volume":"25","author":"S Bayda","year":"2019","journal-title":"Molecules"},{"issue":"2","key":"pone.0337942.ref005","doi-asserted-by":"crossref","first-page":"100694","DOI":"10.1016\/j.jsamd.2024.100694","article-title":"Applications of nanomaterial technology in biosensing","volume":"9","author":"Y Fu","year":"2024","journal-title":"J Sci Adv Materials Devices"},{"issue":"1","key":"pone.0337942.ref006","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1186\/s12951-022-01477-8","article-title":"Nanoparticle classification, physicochemical properties, characterization, and applications: a comprehensive review for biologists","volume":"20","author":"N Joudeh","year":"2022","journal-title":"J Nanobiotechnology"},{"issue":"11","key":"pone.0337942.ref007","doi-asserted-by":"crossref","first-page":"6415","DOI":"10.1007\/s12649-024-02445-z","article-title":"Green synthesis and characterisation of iron oxide nanoparticle using ziziphus oenoplia fruit extract: A biomedical and environmental potential","volume":"15","author":"K Selvam","year":"2024","journal-title":"Waste Biomass Valor"},{"key":"pone.0337942.ref008","doi-asserted-by":"crossref","first-page":"131379","DOI":"10.1016\/j.matlet.2021.131379","article-title":"Annona reticulata leaves-assisted synthesis of zinc oxide nanoparticles and assessment of cytotoxicity and photocatalytic impact","volume":"309","author":"K Selvam","year":"2022","journal-title":"Materials Letters"},{"key":"pone.0337942.ref009","doi-asserted-by":"crossref","first-page":"128087","DOI":"10.1016\/j.jcrysgro.2025.128087","article-title":"Annona reticulata fruit-based phytosynthesis of zinc oxide nanoparticles and assess antioxidant, antibacterial and photocatalytic degradation of textile dyes","volume":"654","author":"T Chinnathambi","year":"2025","journal-title":"J Cryst Growth"},{"issue":"7","key":"pone.0337942.ref010","doi-asserted-by":"crossref","first-page":"2393","DOI":"10.3390\/ma15072393","article-title":"Onion peel waste mediated-green synthesis of zinc oxide nanoparticles and their phytotoxicity on mung bean and wheat plant growth","volume":"15","author":"S Modi","year":"2022","journal-title":"Materials (Basel)"},{"issue":"4","key":"pone.0337942.ref011","doi-asserted-by":"crossref","first-page":"2837","DOI":"10.1007\/s13204-021-02148-0","article-title":"Photocatalytic degradation of malachite green and antibacterial potential of biomimetic-synthesized zirconium oxide nanoparticles using Annona reticulata leaf extract","volume":"13","author":"K Selvam","year":"2022","journal-title":"Appl Nanosci"},{"issue":"1","key":"pone.0337942.ref012","doi-asserted-by":"crossref","first-page":"101774","DOI":"10.1016\/j.jscs.2023.101774","article-title":"Unlocking nature\u2019s potential: Green synthesis of ZnO nanoparticles and their multifaceted applications \u2013 A concise overview","volume":"28","author":"M Gadewar","year":"2024","journal-title":"J Saudi Chem Soc"},{"issue":"4","key":"pone.0337942.ref013","doi-asserted-by":"crossref","first-page":"1193","DOI":"10.3390\/pr11041193","article-title":"Zinc oxide nanoparticles: Synthesis, characterization, modification, and applications in food and agriculture","volume":"11","author":"X-Q Zhou","year":"2023","journal-title":"Processes"},{"issue":"3","key":"pone.0337942.ref014","article-title":"Green synthesis of zinc oxide nanoparticles from plant extract: A review","volume":"8","year":"2019","journal-title":"Int J Green Herbal Chem"},{"issue":"3","key":"pone.0337942.ref015","doi-asserted-by":"crossref","first-page":"1875","DOI":"10.3390\/ijms24031875","article-title":"Traditional vs. Microfluidic Synthesis of ZnO Nanoparticles","volume":"24","author":"ML Popa","year":"2023","journal-title":"Int J Mol Sci"},{"issue":"2","key":"pone.0337942.ref016","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1007\/s10311-023-01682-3","article-title":"Synthesis of green nanoparticles for energy, 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typha domingensis pers. to ameliorate health pathologies: In Vitro and In silico approaches","volume":"2022","author":"R Dilshad","year":"2022","journal-title":"Biomed Res Int"},{"key":"pone.0337942.ref020","doi-asserted-by":"crossref","first-page":"1273191","DOI":"10.3389\/fchem.2023.1273191","article-title":"Phytochemical characterization of Typha domingensis and the assessment of therapeutic potential using in vitro and in vivo biological activities and in silico studies","volume":"11","author":"R Dilshad","year":"2023","journal-title":"Front Chem"},{"key":"pone.0337942.ref021","doi-asserted-by":"crossref","unstructured":"Al-Kalifawi EJ, Al-Azzawi YJ, kat Al-Fartousi K, Mezher Musa H. 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IntechOpen"},{"key":"pone.0337942.ref033","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1016\/B978-0-12-800723-5.00034-6","article-title":"Zinc oxide nanoparticles for food packaging applications","author":"PJP Espitia","year":"2016","journal-title":"Antimicrobial Food Packaging. Elsevier"},{"issue":"5","key":"pone.0337942.ref034","doi-asserted-by":"crossref","first-page":"1447","DOI":"10.1007\/s11947-012-0797-6","article-title":"Zinc oxide nanoparticles: synthesis, antimicrobial activity and food packaging applications","volume":"5","author":"PJP Espitia","year":"2012","journal-title":"Food Bioprocess Technol"},{"key":"pone.0337942.ref035","doi-asserted-by":"crossref","first-page":"116567","DOI":"10.1016\/j.molliq.2021.116567","article-title":"Green synthesis of ZnO nanoparticles from Syzygium Cumini leaves extract with robust photocatalysis applications","volume":"335","author":"H Sadiq","year":"2021","journal-title":"J Molecular Liquids"},{"key":"pone.0337942.ref036","unstructured":"Oludare O, Bamidele O. Phytochemical screening of ten Nigerian medicinal plants. www.allsubjectjournal.com"},{"issue":"3","key":"pone.0337942.ref037","doi-asserted-by":"crossref","first-page":"100038","DOI":"10.1016\/j.ccmp.2022.100038","article-title":"Exploration of pharmacological and toxicological properties of aerial parts of blumea lacera, a common weed in Bangladesh","volume":"2","author":"P Kundu","year":"2022","journal-title":"Clinical Complementary Med Pharmacol"},{"issue":"4","key":"pone.0337942.ref038","doi-asserted-by":"crossref","first-page":"2358","DOI":"10.1016\/j.jksus.2020.03.014","article-title":"Green synthesis, characterization and anti microbial activities of ZnO nanoparticles using Euphorbia hirta leaf extract","volume":"32","author":"W Ahmad","year":"2020","journal-title":"J King Saud Univ Sci"},{"key":"pone.0337942.ref039","doi-asserted-by":"crossref","first-page":"114577","DOI":"10.1016\/j.jep.2021.114577","article-title":"Metabolite profiling, anti-inflammatory, analgesic potentials of edible herb Colocasia gigantea and molecular docking study against COX-II enzyme","volume":"281","author":"MNH Zilani","year":"2021","journal-title":"J Ethnopharmacol"},{"key":"pone.0337942.ref040","doi-asserted-by":"crossref","first-page":"925494","DOI":"10.1155\/2014\/925494","article-title":"Zinc oxide nanoparticles for revolutionizing agriculture: Synthesis and applications","volume":"2014","author":"S Sabir","year":"2014","journal-title":"ScientificWorldJournal"},{"key":"pone.0337942.ref041","doi-asserted-by":"crossref","first-page":"101441","DOI":"10.1016\/j.rechem.2024.101441","article-title":"Green synthesis of ZnO NPs using aqueous extract of Epipremnum aureum leave: Photocatalytic degradation of Congo red","volume":"7","author":"RS Brishti","year":"2024","journal-title":"Results Chem"},{"issue":"1","key":"pone.0337942.ref042","doi-asserted-by":"crossref","first-page":"200","DOI":"10.22159\/ajpcr.2019.v12i1.28808","article-title":"In vitro analysis: The antimicrobial and antioxidant activity of zinc oxide nanoparticles from curcuma longa","volume":"12","author":"V Jacob","year":"2019","journal-title":"Asian J Pharm Clin Res"},{"issue":"1","key":"pone.0337942.ref043","doi-asserted-by":"crossref","first-page":"65","DOI":"10.5114\/bta.2021.103763","article-title":"Biosynthesis of zinc oxide nanoparticles using Fumaria parviflora extract and evaluation of their antibacterial and antioxidant activities","volume":"102","author":"B Dousti","year":"2021","journal-title":"BioTechnologia (Pozn)"},{"key":"pone.0337942.ref044","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.onano.2018.08.001","article-title":"Synthesis and characterization of zinc oxide nanoparticles using tuber extract of anchote (Coccinia abyssinica (Lam.) Cong.) for antimicrobial and antioxidant activity assessment","volume":"3","author":"T Safawo","year":"2018","journal-title":"OpenNano"},{"issue":"8","key":"pone.0337942.ref045","doi-asserted-by":"crossref","first-page":"085005","DOI":"10.1088\/2053-1591\/ad6ca9","article-title":"Antioxidant response of Calendula officinalis L. assisted synthesized zinc oxide nanoparticles","volume":"11","author":"AK Tiwari","year":"2024","journal-title":"Mater Res Express"},{"issue":"12","key":"pone.0337942.ref046","doi-asserted-by":"crossref","first-page":"4071","DOI":"10.1128\/JCM.01088-08","article-title":"Evaluation of the disk diffusion method compared to the microdilution method in susceptibility testing of anidulafungin against filamentous fungi","volume":"46","author":"CM Maida","year":"2008","journal-title":"J Clin Microbiol"},{"key":"pone.0337942.ref047","unstructured":"Onyebum IE, Charles N, Chukwudi F. In vitro antimicrobial potential of Coturnix japonica egg shell and membrane powder. 2017. https:\/\/www.researchgate.net\/publication\/324416149"},{"key":"pone.0337942.ref048","doi-asserted-by":"crossref","DOI":"10.1093\/biohorizons\/hzy019","article-title":"Phytochemical screening and in vitro antimicrobial activities of Mimosa invisa Mart. leaves and stems","volume":"11","author":"HC Chukwudi","year":"2018","journal-title":"Bioscience Horizons Int J Student Res"},{"issue":"14","key":"pone.0337942.ref049","doi-asserted-by":"crossref","first-page":"1789","DOI":"10.1101\/gad.1074103","article-title":"Recruitment of Thr 319-phosphorylated Ndd1p to the FHA domain of Fkh2p requires Clb kinase activity: A mechanism for CLB cluster gene activation","volume":"17","author":"D Reynolds","year":"2003","journal-title":"Genes Dev"},{"issue":"3","key":"pone.0337942.ref050","doi-asserted-by":"crossref","first-page":"265","DOI":"10.5423\/PPJ.OA.02.2023.0035","article-title":"Antifungal activity of green and chemically synthesized ZnO nanoparticles against alternaria citri, the causal agent citrus black rot","volume":"39","author":"HS Elshafie","year":"2023","journal-title":"Plant Pathol J"},{"issue":"1","key":"pone.0337942.ref051","article-title":"Review in green synthesis mechanisms, application, and future prospects for Garcinia mangostana L. (mangosteen)-derived nanoparticles","volume":"14","author":"YB Chan","year":"2025","journal-title":"Nanotechnology Reviews"},{"issue":"1","key":"pone.0337942.ref052","article-title":"Green synthesis and characterization of CuO\/ZnO nanocomposite using Musa acuminata leaf extract for cytotoxic studies on colorectal cancer cells (HCC2998)","volume":"13","author":"YJ Wong","year":"2024","journal-title":"Green Processing and Synthesis"},{"key":"pone.0337942.ref053","doi-asserted-by":"crossref","first-page":"2931","DOI":"10.1016\/j.jmrt.2022.08.028","article-title":"Synthesis, characterization, and preliminary in vitro antibacterial evaluation of ZnO nanoparticles derived from soursop (Annona muricata L.) leaf extract as a green reducing agent","volume":"20","author":"V Selvanathan","year":"2022","journal-title":"J Materials Res Technol"},{"issue":"4","key":"pone.0337942.ref054","doi-asserted-by":"crossref","first-page":"247","DOI":"10.3390\/nano8040247","article-title":"Nanoscale zinc oxide particles for improving the physiological and sanitary quality of a mexican landrace of red maize","volume":"8","author":"J Estrada-Urbina","year":"2018","journal-title":"Nanomaterials (Basel)"},{"issue":"4","key":"pone.0337942.ref055","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1007\/s10876-014-0806-1","article-title":"Facile Synthesis of Quasi Spherical ZnO Nanoparticles with excellent photocatalytic activity","volume":"26","author":"M Pudukudy","year":"2014","journal-title":"J Clust Sci"},{"key":"pone.0337942.ref056","first-page":"1399","article-title":"Synthesis and characterization of a narrow size distribution of zinc oxide nanoparticles","volume":"6","author":"AK Zak","year":"2011","journal-title":"Int J Nanomedicine"},{"issue":"6","key":"pone.0337942.ref057","doi-asserted-by":"crossref","first-page":"1558","DOI":"10.3390\/nano11061558","article-title":"Cinnamomum tamala leaf extract stabilized Zinc Oxide nanoparticles: A promising photocatalyst for methylene blue degradation","volume":"11","author":"S Narath","year":"2021","journal-title":"Nanomaterials (Basel)"},{"issue":"1","key":"pone.0337942.ref058","doi-asserted-by":"crossref","first-page":"10924","DOI":"10.1038\/s41598-021-90408-y","article-title":"Statistical optimization of experimental parameters for extracellular synthesis of zinc oxide nanoparticles by a novel haloalaliphilic Alkalibacillus sp.W7","volume":"11","author":"HMH Al-Kordy","year":"2021","journal-title":"Sci Rep"},{"issue":"3","key":"pone.0337942.ref059","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1007\/s40097-018-0271-8","article-title":"Biosynthesis, characterization, and antibacterial activity of zinc oxide nanoparticles derived from Bauhinia tomentosa leaf extract","volume":"8","author":"G Sharmila","year":"2018","journal-title":"J Nanostruct Chem"},{"key":"pone.0337942.ref060","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.proeng.2016.06.483","article-title":"Plant Extract Mediated of ZnO Nanoparticles by Using Ethanol Extract of Mimosa Pudica Leaves and Coffee Powder","volume":"148","author":"I Fatimah","year":"2016","journal-title":"Procedia Engineering"},{"key":"pone.0337942.ref061","doi-asserted-by":"crossref","first-page":"1","DOI":"10.5402\/2012\/372505","article-title":"Synthesis, characterization, and spectroscopic properties of ZnO nanoparticles","volume":"2012","author":"S Talam","year":"2012","journal-title":"ISRN Nanotechnology"},{"issue":"3","key":"pone.0337942.ref062","doi-asserted-by":"crossref","first-page":"275","DOI":"10.3390\/catal15030275","article-title":"Green synthesis and characterization of copper oxide nanoparticles from durian (durio zibethinus) husk for environmental applications","volume":"15","author":"Y-P Liang","year":"2025","journal-title":"Catalysts"},{"issue":"2","key":"pone.0337942.ref063","doi-asserted-by":"crossref","first-page":"886","DOI":"10.1007\/s10904-020-01729-w","article-title":"Green synthesis of biogenic zinc oxide nanoflower as dual agent for photodegradation of an organic dye and tyrosinase inhibitor","volume":"31","author":"AC Ekennia","year":"2020","journal-title":"J Inorg Organomet Polym"},{"issue":"1","key":"pone.0337942.ref064","doi-asserted-by":"crossref","first-page":"23","DOI":"10.15761\/FNN.1000105","article-title":"Photocatalytic degradation of organophosphate pesticides (Chlorpyrifos) using synthesized zinc oxide nanoparticle by membrane filtration reactor under UV irradiation","volume":"1","author":"S Heena Khan","year":"2015","journal-title":"Front Nanosci Nanotech"},{"issue":"8","key":"pone.0337942.ref065","doi-asserted-by":"crossref","DOI":"10.1007\/s42452-024-06049-z","article-title":"Spectroscopic investigations of green synthesized zinc oxide nanoparticles (ZnO NPs): antioxidant and antibacterial activity","volume":"6","author":"AK Tiwari","year":"2024","journal-title":"Discov Appl Sci"},{"key":"pone.0337942.ref066","article-title":"FT-IR and EDS analysis of the seaweeds Sargassum wightii (brown algae) and Gracilaria corticata (red algae)","author":"S Kannan","year":"2014","journal-title":"Int J Curr Microbiol App Sci"},{"issue":"15","key":"pone.0337942.ref067","doi-asserted-by":"crossref","first-page":"5421","DOI":"10.3390\/ma16155421","article-title":"Impact of diverse parameters on the physicochemical characteristics of green-synthesized zinc oxide-copper oxide nanocomposites derived from an aqueous extract of garcinia mangostana L. Leaf","volume":"16","author":"YB Chan","year":"2023","journal-title":"Materials (Basel)"},{"issue":"12","key":"pone.0337942.ref068","doi-asserted-by":"crossref","first-page":"1864","DOI":"10.3390\/coatings12121864","article-title":"Antifungal properties of zinc oxide nanoparticles on Candida albicans","volume":"12","author":"S Djearamane","year":"2022","journal-title":"Coatings"},{"key":"pone.0337942.ref069","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.saa.2015.02.041","article-title":"Synthesis, characteristics and antimicrobial activity of ZnO nanoparticles","volume":"144","author":"AC Janaki","year":"2015","journal-title":"Spectrochim Acta A Mol Biomol Spectrosc"},{"key":"pone.0337942.ref070","first-page":"199","article-title":"Green Nanofluids. Lubricants from Renewable Feedstocks","author":"A Bhardwaj","year":"2024"},{"key":"pone.0337942.ref071","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.apsusc.2015.03.176","article-title":"Green synthesis, characterization and antimicrobial activities of zinc oxide nanoparticles from the leaf extract of Azadirachta indica (L.)","volume":"345","author":"K Elumalai","year":"2015","journal-title":"Appl Surface Sci"},{"key":"pone.0337942.ref072","article-title":"Green synthesis of ZnO nanoparticles by Calotropis gigantea","author":"S Hiremath","journal-title":"Int J Current Eng Technol"},{"issue":"8","key":"pone.0337942.ref073","doi-asserted-by":"crossref","first-page":"085005","DOI":"10.1088\/2053-1591\/ad6ca9","article-title":"Antioxidant response of Calendula officinalis L. assisted synthesized zinc oxide nanoparticles","volume":"11","author":"AK Tiwari","year":"2024","journal-title":"Mater Res Express"},{"issue":"8","key":"pone.0337942.ref074","doi-asserted-by":"crossref","first-page":"1444","DOI":"10.3390\/antiox11081444","article-title":"Green Synthesis and Characterization of ZnO Nanoparticles Using Pelargonium odoratissimum (L.) Aqueous Leaf Extract and Their Antioxidant, Antibacterial and Anti-inflammatory Activities","volume":"11","author":"AS Abdelbaky","year":"2022","journal-title":"Antioxidants (Basel)"},{"key":"pone.0337942.ref075","article-title":"Eichhornia Crassipes Plant Extract Templated Green Synthesis of Cr 2O 3\/ZnO Composite Catalyst for the Degradation of Organic Dye","author":"OA Zelekew","year":"2021","journal-title":"SSRN Electronic J"},{"issue":"45","key":"pone.0337942.ref076","doi-asserted-by":"crossref","first-page":"23405","DOI":"10.1039\/C4RA02973A","article-title":"Heterogeneous phase formation in diluted magnetic semiconducting Zn1\u2212x\u2212yCoxAlyO (CAZO) nanoparticles","volume":"4","author":"R Siddheswaran","year":"2014","journal-title":"RSC Adv"},{"issue":"5","key":"pone.0337942.ref077","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1139\/cjc-2016-0456","article-title":"Enhancing the removal of methylene blue by modified ZnO nanoparticles: kinetics and equilibrium studies","volume":"95","author":"TM Elmorsi","year":"2017","journal-title":"Can J Chem"},{"key":"pone.0337942.ref078","unstructured":"Sieber I, Wanderka N, Urban I, Do \u00c8rfel I, Schierhorn E, Fenske F, et al. Electron microscopic characterization of reactively sputtered ZnO \u00aelms with different Al-doping levels."},{"issue":"12","key":"pone.0337942.ref079","doi-asserted-by":"crossref","first-page":"1601","DOI":"10.1007\/s12648-018-1246-9","article-title":"Study of Ni:CeO2 nanoparticles for efficient photodegradation of methylene blue by sun light irradiation","volume":"92","author":"V Ramasamy","year":"2018","journal-title":"Indian J Phys"},{"key":"pone.0337942.ref080","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1016\/j.msec.2015.01.042","article-title":"Synthesis of cerium oxide nanoparticles using Gloriosa superba L. leaf extract and their structural, optical and antibacterial properties","volume":"49","author":"A Arumugam","year":"2015","journal-title":"Mater Sci Eng C Mater Biol Appl"},{"issue":"40","key":"pone.0337942.ref081","doi-asserted-by":"crossref","first-page":"23899","DOI":"10.1039\/D0RA04926C","article-title":"Green synthesis of ZnO nanoparticles using orange fruit peel extract for antibacterial activities","volume":"10","author":"TU Doan Thi","year":"2020","journal-title":"RSC Adv"},{"key":"pone.0337942.ref082","unstructured":"Kumar M, Kumar S, Sharma N, Parveen Z, Kaur J, Sharma N. Facial synthesis of nano sized ZnO by hydrothermal method. 2015. https:\/\/www.researchgate.net\/publication\/276145994"},{"key":"pone.0337942.ref083","doi-asserted-by":"crossref","DOI":"10.1063\/1.5052092","article-title":"Thermal decomposition route to synthesize ZnO nanoparticles for photocatalytic application","author":"S Jayswal","year":"2018","journal-title":"AIP Conference Proceedings"},{"key":"pone.0337942.ref084","first-page":"1694","article-title":"Structural and spectroscopic analysis of pure phase hexagonal wurtzite ZnO nanoparticles synthesized by sol-gel","volume":"49","author":"T Ahmad","year":"2022","journal-title":"Materials Today: Proceedings"},{"issue":"35","key":"pone.0337942.ref085","doi-asserted-by":"crossref","first-page":"25409","DOI":"10.1039\/D4RA04132A","article-title":"Green mediated synthesis of cerium oxide nanoparticles by using Oroxylum indicum for evaluation of catalytic and biomedical activity","volume":"14","author":"J Mim","year":"2024","journal-title":"RSC Adv"},{"key":"pone.0337942.ref086","doi-asserted-by":"crossref","first-page":"4642","DOI":"10.3390\/ijms22094642","article-title":"Molecular sciences the chemistry of reactive oxygen species (ROS) Revisited: Outlining their role in biological macromolecules (DNA, Lipids and Proteins) and induced pathologies","volume":"22","author":"C Andr\u00e9s Juan","year":"2021","journal-title":"Int J Mol Sci"},{"key":"pone.0337942.ref087","doi-asserted-by":"crossref","first-page":"105509","DOI":"10.1016\/j.neuint.2023.105509","article-title":"Oxidative stress: A target to treat Alzheimer\u2019s disease and stroke","volume":"165","author":"S Briyal","year":"2023","journal-title":"Neurochem Int"},{"issue":"2","key":"pone.0337942.ref088","doi-asserted-by":"crossref","first-page":"738","DOI":"10.3390\/molecules14020738","article-title":"Ethanolic extract of propolis (EEP) enhances the apoptosis- inducing potential of TRAIL in cancer cells","volume":"14","author":"E Szliszka","year":"2009","journal-title":"Molecules"},{"issue":"3","key":"pone.0337942.ref089","doi-asserted-by":"crossref","DOI":"10.1016\/j.heliyon.2024.e25430","article-title":"Green synthesis of zinc oxide nano particles using Allium cepa L. waste peel extracts and its antioxidant and antibacterial activities","volume":"10","author":"MF Islam","year":"2024","journal-title":"Heliyon"},{"issue":"11","key":"pone.0337942.ref090","doi-asserted-by":"crossref","first-page":"2934","DOI":"10.14233\/ajchem.2020.22971","article-title":"Plant mediated synthesis of zinc oxide nanoparticles and their potential applications as photocatalyst and antibacterial agents","volume":"32","author":"A Swetharanyam","year":"2020","journal-title":"Asian J Chem"},{"key":"pone.0337942.ref091","doi-asserted-by":"crossref","first-page":"101298","DOI":"10.1016\/j.fpsl.2024.101298","article-title":"Innovative use of chitosan\/ZnO NPs bio-nanocomposites for sustainable antimicrobial food packaging of poultry meat","volume":"43","author":"S Sasidharan","year":"2024","journal-title":"Food Packaging Shelf Life"},{"key":"pone.0337942.ref092","doi-asserted-by":"crossref","first-page":"9395","DOI":"10.2147\/IJN.S216204","article-title":"Relationship between structure and antimicrobial activity of zinc oxide nanoparticles: An overview","volume":"14","author":"B Lallo da Silva","year":"2019","journal-title":"Int J Nanomedicine"}],"container-title":["PLOS One"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0337942","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T18:37:29Z","timestamp":1768329449000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0337942"}},"subtitle":[],"editor":[{"given":"Karthik","family":"Kannan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,1,13]]},"references-count":92,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,1,13]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0337942","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,13]]}}}