{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:03:03Z","timestamp":1787022183815,"version":"build-2736575974"},"reference-count":29,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T00:00:00Z","timestamp":1766102400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009697","name":"Hashemite University","doi-asserted-by":"publisher","award":["29\/2020"],"award-info":[{"award-number":["29\/2020"]}],"id":[{"id":"10.13039\/501100009697","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>Skin Repair Plus Ointment (Remanco) is an advanced formulation of the traditional Jordanian cream, which has been used for treating burn injuries and has served as an alternative remedy in northern Jordan for several decades. In this study, we sought to investigate the therapeutic effects of Remanco on the healing morphology and molecular alterations associated with burn wounds. This randomized controlled experiment involved 90 Wistar rats (160\u2212170 grams) that were randomly assigned to three groups of 30 rats each. The groups were designated as follows: negative control (burn treated with normal saline), positive control (burn treated with 1% silver sulfadiazine), and tested (burn treated with Remanco cream). Burn injuries were inflicted on the shaved dorsum of the rats using a preheated 30-mm-wide circular copper head device. The burns were treated daily with normal saline, 1% silver sulfadiazine, or Remanco cream for 30 days or until the animals were sacrificed. Gross evaluation, histopathological assessment, and RT-PCR Analysis were conducted to evaluate the efficacy of Remanco cream in promoting wound healing. Although there was no statistically significant difference in wound area measurements or histopathological ratings, the cosmetic appearance of the wound area exhibited a significant improvement in the Remanco cream-treated group. Molecular investigations revealed that the Remanco-treated group demonstrated a significant increase in the levels of two essential growth factors: TGF-beta on day 3 and IGF-1 on day 9, with a p-value less than 0.05. In summary, Remanco cream holds potential for enhancing wound healing in animal models, but further studies are necessary to investigate its potential clinical applications.<\/jats:p>","DOI":"10.1371\/journal.pone.0338305","type":"journal-article","created":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T18:29:30Z","timestamp":1766168970000},"page":"e0338305","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Therapeutic effects of skin repair plus ointment on molecular and morphological changes in burn wounds: A rat model"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5772-3830","authenticated-orcid":true,"given":"Foad","family":"Alzoughool","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Borhan","family":"Al-Zghoul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wael","family":"Hananeh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manar","family":"Atoum","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reman","family":"Alnemrat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Alzghool","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmad M.","family":"Al-Bashaireh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yousef","family":"Aljawarneh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamad","family":"Mayyas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2025,12,19]]},"reference":[{"issue":"8","key":"pone.0338305.ref001","first-page":"463","article-title":"Outpatient burn care: prevention and treatment","volume":"101","author":"JS Lanham","year":"2020","journal-title":"Am Fam Physician"},{"key":"pone.0338305.ref002","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1007\/978-3-030-44766-3_5","article-title":"Epidemiology of Scars and Their Consequences: Burn Scars.","volume-title":"Textbook on Scar Management: State of the Art Management and Emerging Technologies","author":"ME. van Baar","year":"2020"},{"key":"pone.0338305.ref003","doi-asserted-by":"crossref","first-page":"112570","DOI":"10.1016\/j.jep.2020.112570","article-title":"The efficacy of a traditional medicine preparation on second-degree burn wounds in rats","volume":"252","author":"N Ebrahimpour","year":"2020","journal-title":"J Ethnopharmacol"},{"issue":"1","key":"pone.0338305.ref004","first-page":"67","article-title":"The healing effect of sesame oil, camphor and honey on second degree burn wounds in rat","volume":"7","author":"R Vaghardoost","year":"2018","journal-title":"World J Plast Surg"},{"issue":"7","key":"pone.0338305.ref005","doi-asserted-by":"crossref","first-page":"3729","DOI":"10.1002\/fsn3.3407","article-title":"Effects of sesame (Sesamum indicum L.) and bioactive compounds (sesamin and sesamolin) on inflammation and atherosclerosis: a review","volume":"11","author":"E Hadipour","year":"2023","journal-title":"Food Sci Nutr"},{"key":"pone.0338305.ref006","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.ctim.2017.08.001","article-title":"The effect of a beeswax, olive oil and Alkanna tinctoria (L.) 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