{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T12:12:20Z","timestamp":1788264740667,"version":"build-2803163510"},"reference-count":68,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T00:00:00Z","timestamp":1766016000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["APP2033851"],"award-info":[{"award-number":["APP2033851"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014582","name":"School of Life Sciences, University of Technology Sydney","doi-asserted-by":"publisher","award":["SRA 2726229"],"award-info":[{"award-number":["SRA 2726229"]}],"id":[{"id":"10.13039\/501100014582","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014582","name":"School of Life Sciences, University of Technology Sydney","doi-asserted-by":"publisher","award":["AIMI seed funding"],"award-info":[{"award-number":["AIMI seed funding"]}],"id":[{"id":"10.13039\/501100014582","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014582","name":"School of Life Sciences, University of Technology Sydney","doi-asserted-by":"publisher","award":["Seed Funding"],"award-info":[{"award-number":["Seed Funding"]}],"id":[{"id":"10.13039\/501100014582","id-type":"DOI","asserted-by":"publisher"}]},{"name":"International Society for Microbial Ecology","award":["Sponsorship grant"],"award-info":[{"award-number":["Sponsorship grant"]}]},{"DOI":"10.13039\/501100001232","name":"Royal Australasian College of Physicians","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001232","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    While investigating colony morphology variation within clinical isolates of\n                    <jats:italic>Burkholderia pseudomallei<\/jats:italic>\n                    (\n                    <jats:italic>Bp)<\/jats:italic>\n                    isolated from a single clinical infection, we identified two distinct strains (based on their multi-locus sequence type) from\n                    <jats:italic>Bp<\/jats:italic>\n                    TSV292. Reports of simultaneous co-infection are un-common, with only two studies in Thailand documenting such cases: (1) 2 out of 133 cases, and (2) 13 out of 59 patients infected with two to three polyclonal\n                    <jats:italic>Bp<\/jats:italic>\n                    strains exhibiting different genotypes. Here, we present the first comprehensive multi-omics characterization of co-infecting\n                    <jats:italic>Bp<\/jats:italic>\n                    strains isolated from a single infection at a hospital in Townsville, Australia, in 2018. This finding impacts the design of future diagnosis and treatment of\n                    <jats:italic>Bp<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0338333","type":"journal-article","created":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T18:39:56Z","timestamp":1766083196000},"page":"e0338333","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Co-isolation of genetically distinct Burkholderia pseudomallei strains from a single patient in North Queensland"],"prefix":"10.1371","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9482-2360","authenticated-orcid":true,"given":"Pauline M. 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