{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T17:11:17Z","timestamp":1790442677477,"version":"4.1.0"},"reference-count":33,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T00:00:00Z","timestamp":1771891200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100013281","name":"Joint Programming Initiative on Antimicrobial Resistance","doi-asserted-by":"crossref","award":["178952"],"award-info":[{"award-number":["178952"]}],"id":[{"id":"10.13039\/100013281","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Dean Cumming School of Medicine at the University of Calgary","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>Loop-mediated isothermal amplification (LAMP) is a widely used rapid and affordable molecular DNA amplification method with minimal resource requirements. However, visual interpretation of results is subjective and prone to errors, leading to potential false-positive and negative results. To address this limitation, a machine-learning approach is proposed for automated LAMP classification based on digital images. The approach utilizes You Only Look Once (YOLOv8), a fast and robust object detection algorithm to locate and classify tubes within LAMP images, enabling automated categorization as positive or negative. The trained model achieved a high overall accuracy of 97.4% in classifying LAMP images into positive or negative on the test set. Additionally, the approach had a 95.3% precision and 96.8% recall for positive cases and 93.3% precision and 95.8% recall for negative cases, demonstrating its potential for real-time LAMP diagnosis and enhanced assay performance. This project demonstrated\u00a0platform\u00a0suitability for real-time testing, offering an easy operation and rapid results.<\/jats:p>","DOI":"10.1371\/journal.pone.0339042","type":"journal-article","created":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T18:25:11Z","timestamp":1771957511000},"page":"e0339042","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["You Only Look Once (YOLO) based machine learning algorithm for real-time detection of loop-mediated isothermal amplification (LAMP) diagnostics"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-6623-1109","authenticated-orcid":true,"given":"Biniyam","family":"Mezgebo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-0943-2414","authenticated-orcid":true,"given":"Ryan","family":"Chaffee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. 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