{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T06:57:26Z","timestamp":1770101846661,"version":"3.49.0"},"reference-count":62,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T00:00:00Z","timestamp":1769990400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003093","name":"Ministry of Higher Education, Malaysia","doi-asserted-by":"publisher","award":["FRGS\/1\/2019\/STG05\/MOSTI\/\/3"],"award-info":[{"award-number":["FRGS\/1\/2019\/STG05\/MOSTI\/\/3"]}],"id":[{"id":"10.13039\/501100003093","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    This study investigated the wound healing potential of\n                    <jats:italic>Khaya senegalensis<\/jats:italic>\n                    leaves through a comprehensive approach, including phytochemical, antioxidant, cytotoxicity, migration, and molecular docking analyses. Bioassay-guided isolation identified \u03b2-sitosterol and stigmasterol, with structures confirmed by NMR and FTIR analyses. Phytochemical screening revealed the presence of flavonoids, saponins, triterpenoids, tannins, and steroids, with solvent polarity significantly influencing composition. The ethyl acetate extract exhibited the strongest DPPH radical scavenging (IC\u2085\u2080\u2009=\u20095.6\u2009mg\/mL). The methanol extract had the highest total phenolic content (198.8\u2009mg GAE\/g) while the ethyl acetate had the highest flavonoid content (1,228.1\u2009mg QE\/g). MTT assays confirmed the cytocompatibility of all extracts, fractions, and isolated compounds in HaCaT and HDF cells (&gt;50% viability at \u2264100 \u00b5g\/mL). Scratch assays demonstrated significant, concentration-dependent wound closure, with optimal effects at 12.5 \u03bcg\/mL. The most active fraction (1.2H) and subfractions (1.2.1H, 1.2.3H), as well as the isolated compounds, achieved complete wound closure in HDF cells within 24 hours. Molecular docking revealed strong binding of \u03b2-sitosterol and stigmasterol with TNF-\u03b1 and GSK-3\u03b2, supporting their role in modulating inflammatory and proliferative pathways. These findings highlight\n                    <jats:italic>Khaya senegalensis<\/jats:italic>\n                    as a promising candidate for promoting wound repair, exerting antioxidant, cytocompatible, and pro-migratory effects.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0339051","type":"journal-article","created":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T18:44:42Z","timestamp":1770057882000},"page":"e0339051","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Bioassay-guided fractionation and identification of wound healing active compounds from Khaya senegalensis leaves"],"prefix":"10.1371","volume":"21","author":[{"given":"Akrimi Najihah","family":"Mohd Khamil","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norizamimie","family":"Nordin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamad Afiq Aizuddin","family":"Rosdi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamad Faiz","family":"Hamzah","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohd Hasnan","family":"Mohd Noor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siti Zuraidah","family":"Mohamad Zobir","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amyra","family":"Amat Sain","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad Tasyriq","family":"Che Omar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2447-0897","authenticated-orcid":true,"given":"Mohamad Nurul Azmi","family":"Mohamad Taib","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-9508-5688","authenticated-orcid":true,"given":"Ayunni Salihah","family":"Zalaludin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2026,2,2]]},"reference":[{"issue":"7193","key":"pone.0339051.ref001","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1038\/nature07039","article-title":"Wound repair and regeneration","volume":"453","author":"GC Gurtner","year":"2008","journal-title":"Nature"},{"issue":"265","key":"pone.0339051.ref002","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.3009337","article-title":"Wound 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(Basel)"},{"key":"pone.0339051.ref043","first-page":"195","article-title":"Structural characterization of stigmasterol and \u03b2-sitosterol from the roots of Premna herbacea Roxb","volume":"7","author":"HH Shwe","year":"2019","journal-title":"Int Eur Ext Enable Sci Eng Manag"},{"issue":"1","key":"pone.0339051.ref044","first-page":"88","article-title":"Isolation and characterisation of stigmasterol and \u03b2-sitosterol from Odontonema strictum (Acanthaceae)","volume":"2","author":"LL Pierre","year":"2015","journal-title":"J Innov Pharm Biol Sci"},{"issue":"4","key":"pone.0339051.ref045","doi-asserted-by":"crossref","first-page":"130","DOI":"10.3923\/ijbc.2012.130.135","article-title":"Isolation of Stigmasterol and \u03b2-Sitosterol from Chloroform Extract of Leaves of Corchorus fascicularis Lam","volume":"6","author":"AP Rajput","year":"2012","journal-title":"Int J Biological 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Liang","year":"2007","journal-title":"Nat Protoc"},{"issue":"1","key":"pone.0339051.ref055","doi-asserted-by":"crossref","first-page":"123","DOI":"10.21010\/ajtcam.v14i1.13","article-title":"Evaluation of the anti-inflammatory capacity of beta-sitosterol in rodent assays","volume":"14","author":"R Paniagua-P\u00e9rez","year":"2016","journal-title":"Afr J Tradit Complement Altern Med"},{"issue":"9","key":"pone.0339051.ref056","first-page":"2259","article-title":"Stigmasterol: a comprehensive review","volume":"2","author":"N Kaur","year":"2011","journal-title":"Int J Pharm Sci Res"},{"key":"pone.0339051.ref057","article-title":"WOUND HEALING ACTIVITY OF JATROPHA TANJORENSIS LEAVES","author":"gowdu Viswanathan MB","year":"2018","journal-title":"Univ J Pharm Res"},{"key":"pone.0339051.ref058","doi-asserted-by":"crossref","first-page":"89","DOI":"10.3389\/fphys.2011.00089","article-title":"Transforming growth Factor-\u03b2 and endoglin signaling orchestrate wound 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