{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T19:46:32Z","timestamp":1771875992551,"version":"3.50.1"},"reference-count":36,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T00:00:00Z","timestamp":1771804800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32403023"],"award-info":[{"award-number":["32403023"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015524","name":"China Three Gorges Corporation","doi-asserted-by":"publisher","award":["WWKY-2021-0351"],"award-info":[{"award-number":["WWKY-2021-0351"]}],"id":[{"id":"10.13039\/100015524","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Yichang city science and technology research and development project","award":["B25-1-004"],"award-info":[{"award-number":["B25-1-004"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    The Chinese sturgeon (\n                    <jats:italic>Acipenser sinensis<\/jats:italic>\n                    ), a critically endangered anadromous species, faces severe population declines due to habitat fragmentation and overfishing. This study investigates the effects of dietary Glycyrrhiza polysaccharide (GCP) on growth performance, serum biochemistry, and hepatic transcriptome in juvenile Chinese sturgeon. One hundred and twenty uniform-sized juveniles (365.6\u2009\u00b1\u200986.4\u2009g, 45.2\u2009\u00b1\u20094.1\u2009cm) were randomly allocated to four groups (n\u2009=\u200930; 3\u2009\u00d7\u200910 fish). The experiment consisted of a basal diet supplemented with 0.0% (control), 0.5%, 1.0%, or 2.0% GCP. After 36 days of feeding, significant differences in growth performance were observed among the experimental groups. Compared with control, 2% GCP increased final body weight by 13.1% (699.6\u2009\u00b1\u200945.3\u2009g vs 618.6\u2009\u00b1\u200927.0\u2009g, P\u2009=\u20090.008) and reduced feed conversion ratio by 18% (1.15\u2009\u00b1\u20090.06 vs 1.40\u2009\u00b1\u20090.09, P\u2009=\u20090.003); serum IGF-1 rose 33% (12.52\u2009\u00b1\u20091.71 vs 9.44\u2009\u00b1\u20092.37\u2009ng mL\u2009\u207b\u2009\u00b9, P\u2009=\u20090.012). Hepatic transcriptome profiling identified 400 differentially expressed genes (DEGs) between the control and 2.0% GCP groups (214 up-regulated, 186 down-regulated), with pronounced enrichment in pathways governing energy metabolism, lipid biosynthesis, and cellular proliferation. Transcriptomics (|log\u2082FC|\u2009\u2265\u20091, FDR\u2009&lt;\u20090.01) revealed 400 differentially expressed genes enriched in lipid metabolism and GH-IGF-1 signaling. Key upregulated genes included those involved in lipid synthesis, glycolysis, and growth signaling. The findings demonstrate that dietary supplementation of 2.0% GCP significantly enhances growth performance in juvenile Chinese sturgeon by modulating endocrine signaling and upregulating metabolic pathways, providing a molecular foundation for GCP\u2019s role in improving aquaculture productivity and conservation strategies.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0339813","type":"journal-article","created":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T18:37:57Z","timestamp":1771871877000},"page":"e0339813","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of dietary glycyrrhiza polysaccharide on growth, serum biochemistry, and hepatic transcriptome in juvenile chinese sturgeon (Acipenser sinensis)"],"prefix":"10.1371","volume":"21","author":[{"given":"Jianming","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tian","family":"Tian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"He","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Binzhong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4408-9150","authenticated-orcid":true,"given":"Yacheng","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2026,2,23]]},"reference":[{"key":"pone.0339813.ref001","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.biocon.2015.11.006","article-title":"New 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Meng","year":"2022","journal-title":"Aquaculture Reports"},{"key":"pone.0339813.ref025","doi-asserted-by":"crossref","first-page":"111029","DOI":"10.1016\/j.mce.2020.111029","article-title":"Nutrient regulation of somatic growth in teleost fish. The interaction between somatic growth, feeding and metabolism","volume":"518","author":"LF Canosa","year":"2020","journal-title":"Mol Cell Endocrinol"},{"key":"pone.0339813.ref026","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.cbpa.2012.08.003","article-title":"Peripheral regulation of the growth hormone-insulin-like growth factor system in fish and other vertebrates","volume":"163","author":"KM Reindl","year":"2012","journal-title":"Comp Biochem Physiol A Mol Integr Physiol"},{"key":"pone.0339813.ref027","author":"JM Berg","year":"2002"},{"issue":"12","key":"pone.0339813.ref028","doi-asserted-by":"crossref","first-page":"4338","DOI":"10.1111\/are.15932","article-title":"Effects ofMoringa oleifera\u2010based diets on growth, immunological responses, liver antioxidant biomarkers and expression of immune\u2010related genes in Nile tilapia (Oreochromis niloticus) raised in hapa\u2010in\u2010pond system","volume":"53","author":"MAM El\u2010Son","year":"2022","journal-title":"Aquaculture Research"},{"issue":"7203","key":"pone.0339813.ref029","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1038\/nature07202","article-title":"Integration of metabolism and inflammation by lipid-activated nuclear receptors","volume":"454","author":"SJ Bensinger","year":"2008","journal-title":"Nature"},{"issue":"6","key":"pone.0339813.ref030","doi-asserted-by":"crossref","first-page":"2344","DOI":"10.1111\/anu.13367","article-title":"Effects of tea polyphenols on the growth performance, carbohydrate metabolism of grass carp ( Ctenopharyngodon idellus )","volume":"27","author":"D Yue","year":"2021","journal-title":"Aquacult Nutr"},{"issue":"2","key":"pone.0339813.ref031","doi-asserted-by":"crossref","first-page":"1015","DOI":"10.1111\/raq.12511","article-title":"A global analysis on the systemic effects of antibiotics in cultured fish and their potential human health risk: a review","volume":"13","author":"SM Limbu","year":"2020","journal-title":"Reviews in Aquaculture"},{"key":"pone.0339813.ref032","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/j.fsi.2017.02.027","article-title":"Dietary \u03b2-glucans differentially modulate immune and stress-related gene expression in lymphoid organs from healthy and Aeromonas hydrophila-infected rainbow trout (Oncorhynchus mykiss)","volume":"63","author":"J Douxfils","year":"2017","journal-title":"Fish Shellfish Immunol"},{"key":"pone.0339813.ref033","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.aquaculture.2015.01.010","article-title":"Omega-3 long-chain polyunsaturated fatty acids and aquaculture in perspective","volume":"449","author":"DR Tocher","year":"2015","journal-title":"Aquaculture"},{"key":"pone.0339813.ref034","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/S0044-8486(99)00191-X","article-title":"Lipid nutrition of marine fish during early development: current status and future directions","volume":"179","author":"J Sargent","year":"1999","journal-title":"Aquaculture"},{"key":"pone.0339813.ref035","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1016\/j.aquaculture.2004.02.026","article-title":"Growth, viability and genetic characteristics of GH transgenic coho salmon strains","volume":"236","author":"RH Devlin","year":"2004","journal-title":"Aquaculture"},{"issue":"1","key":"pone.0339813.ref036","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0169991","article-title":"Growth-enhanced transgenic coho salmon (oncorhynchus kisutch) strains have varied success in simulated streams: Implications for risk assessment","volume":"12","author":"RA Leggatt","year":"2017","journal-title":"PLoS One"}],"container-title":["PLOS One"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0339813","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T18:38:02Z","timestamp":1771871882000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0339813"}},"subtitle":[],"editor":[{"given":"Shafaq","family":"Fatima","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2026,2,23]]},"references-count":36,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026,2,23]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0339813","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,23]]}}}