{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T18:03:16Z","timestamp":1793124196902,"version":"4.5.0"},"reference-count":50,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T00:00:00Z","timestamp":1769731200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Fundamental Research Program of Shanxi Province","award":["20210302124229"],"award-info":[{"award-number":["20210302124229"]}]},{"name":"Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi","award":["2021L156"],"award-info":[{"award-number":["2021L156"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Biochemical metabolism and anatomical structure within leaf tissues have been proposed as the two principal mechanisms underpinning the rapid responsiveness of mesophyll conductance (\n                    <jats:italic>g<\/jats:italic>\n                    <jats:sub>m<\/jats:sub>\n                    ) to environmental perturbations; nevertheless, empirical evidence distinguishing which of these factors acts as the dominant driver remains scarce. The response of intra-leaf CO\n                    <jats:sub>2<\/jats:sub>\n                    diffusion conductance including\n                    <jats:italic>g<\/jats:italic>\n                    <jats:sub>m<\/jats:sub>\n                    and stomatal conductance (\n                    <jats:italic>g<\/jats:italic>\n                    <jats:sub>sc<\/jats:sub>\n                    ) to soil nitrogen (N) change in soybean was systematically quantified in leaf biochemical and structural characteristics. Our data revealed that (i) soil N made a positive effect on intra-leaf CO\n                    <jats:sub>2<\/jats:sub>\n                    diffusion and carbon assimilation that\n                    <jats:italic>g<\/jats:italic>\n                    <jats:sub>m<\/jats:sub>\n                    and\n                    <jats:italic>A<\/jats:italic>\n                    <jats:sub>n<\/jats:sub>\n                    (net photosynthetic rate) exhibited a significant positive response to increasing N supplying from 7.5 to 15.0\u2009g urea m\n                    <jats:sup>-2<\/jats:sup>\n                    while\n                    <jats:italic>g<\/jats:italic>\n                    <jats:sub>sc<\/jats:sub>\n                    showed no significant N-dependence. (ii) The enhanced intra-leaf CO\n                    <jats:sub>2<\/jats:sub>\n                    diffusion capacity induced by N application was principally attributable to the increase in\n                    <jats:italic>g<\/jats:italic>\n                    <jats:sub>m<\/jats:sub>\n                    .(iii) The enhancement of biochemical metabolism rather than the modifications in the leaf anatomical structure constituted the predominant mechanism by which N supplementation facilitated CO\n                    <jats:sub>2<\/jats:sub>\n                    diffusion and carbon assimilation in soybean. (iv)Furthermore, the improvement in water use efficiency (WUE) appeared to be more closely linked to aquaporin-mediated water relations, as supported by subsequent correlation analyses.These findings will advance our understanding of the key drivers that shape\n                    <jats:italic>g<\/jats:italic>\n                    <jats:sub>m<\/jats:sub>\n                    responsiveness to abrupt environmental variations.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0340250","type":"journal-article","created":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T18:33:46Z","timestamp":1769798026000},"page":"e0340250","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Biochemical metabolic enhancement acting as a dominant driver in intra-leaf CO2 diffusional response to soil nitrogen supplying in Soybean"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-6010-9782","authenticated-orcid":true,"given":"Qihui","family":"Zuo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyu","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lina","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueer","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fenwu","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,1,30]]},"reference":[{"issue":"9","key":"pone.0340250.ref001","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1641\/B580908","article-title":"Consequences 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