{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T22:52:18Z","timestamp":1769295138362,"version":"3.49.0"},"reference-count":64,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T00:00:00Z","timestamp":1768867200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Key Medical Discipline Project of Joint Logistics Support Force of the People\u00e2\u20ac\u2122s Liberation Army of China"},{"name":"Key Discipline Project of Shandong Province Medical and Health"},{"name":"Shandong Province Medical and Health Technology Development Plan","award":["202204011069"],"award-info":[{"award-number":["202204011069"]}]},{"name":"2023 Ji'nan Science and Technology Innovation Development Program","award":["202317016"],"award-info":[{"award-number":["202317016"]}]},{"name":"2024 Ji'nan Clinical Medicine Science and Technology Innovation Program","award":["202430064"],"award-info":[{"award-number":["202430064"]}]},{"name":"Shandong Provincial Natural Science Foundation General Project","award":["ZR2021MH328"],"award-info":[{"award-number":["ZR2021MH328"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The purpose of this study was to investigate the surgical model of hypothyroidism in rats and the feasibility and stability of the model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Forty-eight adult male Wistar rats were randomly divided into 12 sham-operated rats (control group) and 36 operated rats (18 rats in the group with total thyroidectomy and 18 rats in the group with subtotal thyroidectomy). Thyroid-stimulating hormone (TSH), free thyroxine (FT4), and free triiodothyronine (FT3) serum concentrations were measured prior to surgery and on postoperative days 14, 28 and 90; blood calcium (Ca), blood phosphorus (P), and parathyroid hormone (PTH) concentrations were measured on postoperative day 10; and thyroglobulin (Tg) concentrations were measured on postoperative day 60. The postoperative behavioral status of the rats was comprehensively evaluated on the basis of behavioral indicators.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      There was no significant difference in preoperative TSH, FT4, or FT3 between the control and surgical groups (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&gt;\u20090.05). On postoperative day 10, there were no significant differences in Ca, P, or PTH levels among the groups (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&gt;\u20090.05). On postoperative days 14, 28 and 90, the TSH level was greater, whereas the FT4 and FT3 levels were lower in the surgical group (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05). On the 60th postoperative day, the Tg was significantly lower in the surgical group (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Surgical removal of the thyroid gland can be used to successfully establish a hypothyroid rat model that is stable and reliable and can provide a model basis for hypothyroidism-related research.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pone.0340302","type":"journal-article","created":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T18:44:19Z","timestamp":1768934659000},"page":"e0340302","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Establishment and investigation of a surgical model of 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