{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T20:56:10Z","timestamp":1793134570053,"version":"4.5.0"},"reference-count":51,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T00:00:00Z","timestamp":1769731200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100030927","name":"Roquette Freres","doi-asserted-by":"crossref","award":["CPS-20-255"],"award-info":[{"award-number":["CPS-20-255"]}],"id":[{"id":"10.13039\/100030927","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>Increasing protein content of foods is effective in reducing postprandial hyperglycaemia, but animal protein may exacerbate insulin sensitivity. This single-blind, randomised, crossover study compared the effects of co-ingesting glucose with 10 or 20 g whey protein and glucose with 10 or 20 g pea protein, with a reference product (glucose) on glycaemic and insulinaemic responses in 30 healthy individuals. Blood glucose and plasma insulin were measured at baseline, 15, 30, 45, 60, 90, 120, 150 and 180 minutes after product consumption. The trial was registered with Clinical Trials.gov (NCT04871971). Glucose incremental area under the curve (mmol\/l*min) at 180 minutes was significantly reduced (p\u2009&lt;\u20090.001) for glucose with 20 g pea protein (89.8\u2009\u00b1\u200951.6) and glucose with 20g whey protein (98.5\u2009\u00b1\u200958.0) compared to glucose (143.2\u2009\u00b1\u200974.0). Insulin incremental area under the curve at 180 minutes (\u00b5U\/ml*min) for glucose with 20 g pea protein (4304.56\u2009\u00b1\u20091896.07) was significantly lower (p\u2009&lt;\u20090.001) than glucose with 20g whey protein (6311.81\u2009\u00b1\u20093489.12). This study has shown a superior effect of pea protein over whey protein in reducing glycaemic response, without any excessive increase in insulinaemic response.<\/jats:p>","DOI":"10.1371\/journal.pone.0340386","type":"journal-article","created":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T18:22:50Z","timestamp":1769797370000},"page":"e0340386","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["A randomized controlled trial in healthy participants to compare the insulinogenic effects of whey protein and pea protein co-ingested with glucose"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2065-8443","authenticated-orcid":true,"given":"Pariyarath 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Lonnie","year":"2020","journal-title":"Nutrients"},{"issue":"3","key":"pone.0340386.ref026","doi-asserted-by":"crossref","first-page":"166","DOI":"10.7570\/jomes20028","article-title":"Clinical evidence and mechanisms of high-protein diet-induced weight loss","volume":"29","author":"J Moon","year":"2020","journal-title":"J Obes Metab Syndr"},{"issue":"1","key":"pone.0340386.ref027","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1186\/s12970-014-0064-5","article-title":"Pea proteins oral supplementation promotes muscle thickness gains during resistance training: a double-blind, randomized, Placebo-controlled clinical trial vs. Whey protein","volume":"12","author":"N Babault","year":"2015","journal-title":"J Int Soc Sports Nutr"},{"key":"pone.0340386.ref028","unstructured":"Protein. 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[Cited 2023 February 24]. https:\/\/www.thegrocer.co.uk\/consumer-trends\/gender-bias-health-concerns-and-sports-shy-consumers-10-charts-explaining-uk-attitudes-to-protein\/598311.article"},{"key":"pone.0340386.ref030","article-title":"Research Randomizer (Version 4.0) [Computer software]","author":"GC Urbaniak","year":"2013"},{"key":"pone.0340386.ref031","volume-title":"Food products-determination of the glycaemic index (GI) and recommendation for food classification. 26642","author":"ISO","year":"2010"},{"issue":"1","key":"pone.0340386.ref032","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1186\/1743-7075-9-48","article-title":"The insulinogenic effect of whey protein is partially mediated by a direct effect of amino acids and GIP on \u03b2-cells","volume":"9","author":"A Salehi","year":"2012","journal-title":"Nutr Metab (Lond)"},{"key":"pone.0340386.ref033","doi-asserted-by":"crossref","DOI":"10.1096\/fasebj.31.1_supplement.147.1","article-title":"Glucose, insulin and 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Nutr"},{"issue":"6","key":"pone.0340386.ref046","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1016\/j.cmet.2013.04.008","article-title":"Pharmacology, physiology, and mechanisms of incretin hormone action","volume":"17","author":"JE Campbell","year":"2013","journal-title":"Cell Metab"},{"issue":"12","key":"pone.0340386.ref047","doi-asserted-by":"crossref","first-page":"3746","DOI":"10.3390\/nu12123746","article-title":"Explorative screening of bioactivities generated by plant-based proteins after in vitro static gastrointestinal digestion","volume":"12","author":"C Dugardin","year":"2020","journal-title":"Nutrients"},{"issue":"15","key":"pone.0340386.ref048","doi-asserted-by":"crossref","DOI":"10.3390\/ijms23158365","article-title":"In vivo and in vitro comparison of the DPP-IV inhibitory potential of food proteins from different origins after gastrointestinal digestion","volume":"23","author":"L Fleury","year":"2022","journal-title":"Int J Mol 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