{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T23:16:43Z","timestamp":1768259803170,"version":"3.49.0"},"reference-count":43,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T00:00:00Z","timestamp":1768176000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"state program \u201cEnsuring chemical and biological safety of the Russian Federation\u201d"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Isothermal nucleic acid amplification methods, such as recombinase polymerase amplification (RPA), are becoming increasingly vital as diagnostic platforms for neglected tropical diseases by enabling rapid and accurate detection in under-resourced regions. We developed a real-time RT-RPA assay for Machupo virus (MACV), the causative agent of Bolivian hemorrhagic fever, and directly compared it to a real-time RT-PCR assay targeting the same viral sequence fragment. The methods were evaluated across critical parameters: limit of detection (LOD), tolerance to single-nucleotide substitutions, multiplexing capability, and adaptability to multiple MACV genetic variants. The LOD was identical for both assays: 5\u2009\u00d7\u200910\n                    <jats:sup>3<\/jats:sup>\n                    copies\/ml of armored RNA particles. They differed in terms of input RNA (copies\/reaction): 100 (PCR) versus 20 (RPA). The real-time RT-RPA assay was further validated on a portable device, demonstrating its potential for field-deployability for point-of-care applications.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0340488","type":"journal-article","created":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T18:34:27Z","timestamp":1768242867000},"page":"e0340488","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Isothermal real-time RT-RPA for Machupo virus detection: Field-adaptable sensitivity comparable with laboratory PCR"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3322-2032","authenticated-orcid":true,"given":"Marina A.","family":"Kapitonova","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna V.","family":"Shabalina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Igor S.","family":"Sukhikh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9375-2943","authenticated-orcid":true,"given":"Artemiy A.","family":"Volkov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5500-0169","authenticated-orcid":true,"given":"Vladimir G.","family":"Dedkov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna S.","family":"Dolgova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2026,1,12]]},"reference":[{"key":"pone.0340488.ref001","doi-asserted-by":"crossref","first-page":"102001","DOI":"10.1016\/j.tmaid.2021.102001","article-title":"Bolivian hemorrhagic fever: A narrative review","volume":"40","author":"CR Silva-Ramos","year":"2021","journal-title":"Travel Med Infect Dis"},{"key":"pone.0340488.ref002","doi-asserted-by":"crossref","first-page":"372","DOI":"10.3389\/fimmu.2019.00372","article-title":"Hemorrhagic fever-causing arenaviruses: lethal pathogens and potent immune suppressors","volume":"10","author":"ME Brisse","year":"2019","journal-title":"Front Immunol"},{"issue":"10","key":"pone.0340488.ref003","doi-asserted-by":"crossref","first-page":"2097","DOI":"10.3390\/v4102097","article-title":"Serological assays based on recombinant viral proteins for the diagnosis of arenavirus hemorrhagic fevers","volume":"4","author":"S Fukushi","year":"2012","journal-title":"Viruses"},{"issue":"12","key":"pone.0340488.ref004","doi-asserted-by":"crossref","first-page":"1933","DOI":"10.1016\/j.jiph.2023.10.012","article-title":"Application of multiplex realtime PCR detection for hemorrhagic fever syndrome viruses","volume":"16","author":"Y Choi","year":"2023","journal-title":"J Infect Public Health"},{"key":"pone.0340488.ref005","doi-asserted-by":"crossref","first-page":"107831","DOI":"10.1016\/j.ijid.2025.107831","article-title":"Recombinase polymerase amplification technology for point-of-care diagnosis of neglected tropical diseases","volume":"153","author":"L Hueso","year":"2025","journal-title":"Int J Infect Dis"},{"key":"pone.0340488.ref006","doi-asserted-by":"crossref","unstructured":"Ablordey A, Amissah DA, Aboagye IF, Hatano B, Yamazaki T, Sata T. 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