{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,5]],"date-time":"2026-04-05T05:25:08Z","timestamp":1775366708224,"version":"3.50.1"},"reference-count":59,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T00:00:00Z","timestamp":1769126400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82200560"],"award-info":[{"award-number":["82200560"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82100532"],"award-info":[{"award-number":["82100532"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LQ22H030010"],"award-info":[{"award-number":["LQ22H030010"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The gut microbiome plays a pivotal role in the development and progression of liver disease. Liver sinusoidal endothelial cells (LSECs), as the first hepatic barrier exposed to blood from the portal circulation, may be influenced by gut-derived microbiota and their byproducts. This study aimed to investigate the interaction between gut microbiota and LSECs and to clarify how this interaction impacts the progression of liver cirrhosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Liver cirrhosis was induced by carbon tetrachloride (CCl\n                      <jats:sub>4<\/jats:sub>\n                      ) injection and bile duct ligation (BDL). CCl\n                      <jats:sub>4<\/jats:sub>\n                      and BDL mice were administered rifaximin. The primary LSECs were isolated from mice and treated with LPS. 16S rRNA sequencing was conducted to examine changes in the gut microbiota of cirrhotic mice following rifaximin treatment.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Rifaximin attenuated liver fibrosis and LSEC dysfunction in CCl\n                      <jats:sub>4<\/jats:sub>\n                      and BDL mice. Liver cirrhosis induced remarkable changes in the gut microbiome while rifaximin treatment could partially reverse these alterations. Serum lipopolysaccharides (LPS) level was elevated in cirrhotic mice, while reduced following rifaximin treatment. Furthermore, LPS treatment could induce LSEC dysfunction by inhibiting eNOS mRNA expression, which was attenuated by TLR4 inhibitor, indicating that TLR4 signaling was involved in LPS-induced LSEC dysfunction.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Intestinal microbiota dysbiosis allows more LPS to enter the portal circulation, which may in turn exacerbate LSEC dysfunction and liver fibrosis. Intestinal decontamination with rifaximin improves LSEC function and alleviates liver fibrosis, a process linked to the reconstruction of the gut microbiome and a reduction in gut-derived LPS.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pone.0340664","type":"journal-article","created":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T18:34:50Z","timestamp":1769193290000},"page":"e0340664","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Intestinal decontamination with rifaximin attenuates LSEC dysfunction and liver fibrosis in 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Friedman","year":"2008","journal-title":"Gastroenterology"},{"issue":"3","key":"pone.0340664.ref003","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1038\/s41575-020-00376-3","article-title":"Utilizing the gut microbiome in decompensated cirrhosis and acute-on-chronic liver failure","volume":"18","author":"J Trebicka","year":"2021","journal-title":"Nat Rev Gastroenterol Hepatol"},{"key":"pone.0340664.ref004","article-title":"The microbiota in cirrhosis and its role in hepatic decompensation","author":"J Trebicka","year":"2021","journal-title":"J Hepatol"},{"issue":"6","key":"pone.0340664.ref005","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1053\/j.gastro.2014.01.020","article-title":"Interactions between the intestinal microbiome and liver diseases","volume":"146","author":"B 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disease","volume":"7","author":"AL Harte","year":"2010","journal-title":"J Inflamm (Lond)"},{"issue":"3","key":"pone.0340664.ref043","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1002\/hep.27779","article-title":"Systemic inflammatory response and serum lipopolysaccharide levels predict multiple organ failure and death in alcoholic hepatitis","volume":"62","author":"J Michelena","year":"2015","journal-title":"Hepatology"},{"issue":"1","key":"pone.0340664.ref044","first-page":"11","article-title":"Endotoxemia, encephalopathy, and mortality in cirrhotic patients","volume":"82","author":"LM Bigatello","year":"1987","journal-title":"Am J Gastroenterol"},{"issue":"11","key":"pone.0340664.ref045","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1111\/1751-2980.12294","article-title":"Does low-dose rifaximin ameliorate endotoxemia in patients with liver cirrhosis: a prospective study","volume":"16","author":"X Zeng","year":"2015","journal-title":"J Dig 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