{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T03:35:32Z","timestamp":1785900932809,"version":"3.56.0"},"reference-count":50,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T00:00:00Z","timestamp":1769472000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["2022-67015-36199"],"award-info":[{"award-number":["2022-67015-36199"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000199","name":"U.S. Department of Agriculture","doi-asserted-by":"publisher","award":["2024-67034-42236"],"award-info":[{"award-number":["2024-67034-42236"]}],"id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Colostrum (COL) intake relates to growth trajectory of pigs, which may be driven by differences in nutrient level, bioactive factors, or both. This study\u2019s objective was to determine the effect of feeding COL versus milk replacer (MR) at high and low doses [20% or 10% of birth body weight (BBW)] on 24\u2009h weight gain, body temperature, circulating levels of glucose, protein, immunocrit, free amino acids, lipids and small metabolites. At birth, gilt piglets (n\u2009=\u200957) were assigned to: pooled COL fed at 20% (COL20) or 10% of BBW (COL10); MR fed at 20% (MR20) or 10% of BBW (MR10); stay on sow (SOS) to suckle COL; or immediately euthanized at birth (zero hour; ZH). COL20, COL10, MR20, and MR10 were bottle fed diets every 2\u2009h for 24\u2009h, then piglets were euthanized, and blood collected. Serum samples were analyzed for glucose and protein using a colorimetric assay, immunocrit using the immunocrit ratio, free amino acids using liquid chromatography mass spectrometry, and lipidome using multiple reaction monitoring profiling. Dose affected glucose (\n                    <jats:italic>P<\/jats:italic>\n                    \u2009=\u20090.02), being greater in 20% treatments than 10%. Diet and dose impacted immunocrit (\n                    <jats:italic>P<\/jats:italic>\n                    \u2009&lt;\u20090.0001) and protein (\n                    <jats:italic>P<\/jats:italic>\n                    \u2009=\u20090.04), being highest in COL20, intermediate in COL10, and lowest in MR treatments. Diet type impacted all free amino acids (\n                    <jats:italic>P<\/jats:italic>\n                    \u2009&lt;\u20090.05). Diet and dose impacted concentration of triacylglycerols (\n                    <jats:italic>P<\/jats:italic>\n                    \u2009=\u20090.0003), cholesterol esters (diet\n                    <jats:italic>P<\/jats:italic>\n                    \u2009=\u20090.001; dose\n                    <jats:italic>P<\/jats:italic>\n                    \u2009=\u20090.05), and only diet impacted phospholipids (\n                    <jats:italic>P<\/jats:italic>\n                    \u2009=\u20090.04). Diet impacted individual triacylglycerol profile, with fatty acids longer and more unsaturated in COL than MR. Piglets on SOS, COL20, MR20 gained weight and maintained rectal temperature, whereas COL10 and MR10 lost weight and had lower temperatures (\n                    <jats:italic>P<\/jats:italic>\n                    \u2009&lt;\u20090.05). These data and previous studies indicate colostral proteins remain intact post-absorption preventing use for energy. Due to relatively high lactose in MR and circulating metabolite profiles, we postulated piglets fed MR prioritized glycolysis to support growth, whereas COL-fed depended on fatty acid oxidation. Further studies are warranted to understand how absorptive and post-absorptive metabolism respond to diet type and dose and contribute to circulating profiles of nutrients and metabolites.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0341179","type":"journal-article","created":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T18:41:22Z","timestamp":1769539282000},"page":"e0341179","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Impact of level of nutritional dose and diet specific components of colostrum in promoting 24\u2009h gain, circulating lipid profile, and circulating levels of immunocrit, proteins, glucose, and free amino acids in neonatal gilt piglets"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0176-9500","authenticated-orcid":true,"given":"Linda 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Garcia","family":"Reis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9729-5789","authenticated-orcid":true,"given":"KaLynn","family":"Harlow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-3093-635X","authenticated-orcid":true,"given":"Addison","family":"Hill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michayla","family":"Dinn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma","family":"Shelton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evy","family":"Tobolski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caeden","family":"Meade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Han-Hallett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3988-6454","authenticated-orcid":true,"given":"Christina 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FITC-dextran) in the neonatal pig: enhancing effect of colostrum, proteins and proteinase inhibitors","volume":"47","author":"BR Westr\u00f6m","year":"1985","journal-title":"Biol Neonate"},{"key":"pone.0341179.ref015","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.mce.2011.10.008","article-title":"Lactocrine programming of female reproductive tract development: environmental connections to the reproductive continuum","volume":"354","author":"FF Bartol","year":"2012","journal-title":"Mol Cell Endocrinol"},{"key":"pone.0341179.ref016","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1111\/j.1749-6632.2009.03834.x","article-title":"Milk-borne relaxin and the lactocrine hypothesis for maternal programming of neonatal tissues","volume":"1160","author":"CA Bagnell","year":"2009","journal-title":"Ann N Y Acad Sci"},{"key":"pone.0341179.ref017","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.livsci.2012.03.010","article-title":"Colostrum intake: 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Impact level nutritional dose colostrum versus milk replacer promoting 24\u2009h gain, circulating lipid profile, circulating levels immunocrit, proteins, glucose, free amino acids neonatal gilt piglets. 2025. https:\/\/doi.org\/10.4231\/KN6Y-7S80"},{"issue":"1","key":"pone.0341179.ref020","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.tvjl.2012.06.009","article-title":"A simple novel measure of passive transfer of maternal immunoglobulin is predictive of preweaning mortality in piglets","volume":"195","author":"JL Vallet","year":"2013","journal-title":"Vet J"},{"issue":"10","key":"pone.0341179.ref021","doi-asserted-by":"crossref","first-page":"2867","DOI":"10.3390\/ani11102867","article-title":"Mammary Development in Gilts at One Week Postnatal Is Related to Plasma Lysine Concentration at 24\u2009h after Birth, but Not Colostrum Dose","volume":"11","author":"B Bitsie","year":"2021","journal-title":"Animals 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Tech"},{"key":"pone.0341179.ref025","doi-asserted-by":"crossref","first-page":"105048","DOI":"10.1016\/j.chemphyslip.2021.105048","article-title":"Multiple reaction monitoring profiling (MRM profiling): Small molecule exploratory analysis guided by chemical functionality","volume":"235","author":"Z Xie","year":"2021","journal-title":"Chem Phys Lipids"},{"key":"pone.0341179.ref026","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkad896","article-title":"LIPID MAPS: update to databases and tools for the lipidomics community","volume":"52","author":"MJ Conroy","year":"2024","journal-title":"Nucleic Acids Res"},{"key":"pone.0341179.ref027","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkae253","article-title":"MetaboAnalyst 6.0: towards a unified platform for metabolomics data processing, analysis and interpretation","volume":"52","author":"Z Pang","year":"2024","journal-title":"Nucleic Acids Res"},{"issue":"1","key":"pone.0341179.ref028","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1080\/00401706.1977.10489493","article-title":"Detection of Influential Observation in Linear Regression","volume":"19","author":"RD Cook","year":"1977","journal-title":"Technometrics"},{"issue":"12","key":"pone.0341179.ref029","doi-asserted-by":"crossref","first-page":"2755","DOI":"10.3390\/ijms18122755","article-title":"Metabolic Pathways of the Warburg Effect in Health and Disease: Perspectives of Choice, Chain or Chance","volume":"18","author":"JS Burns","year":"2017","journal-title":"Int J Mol Sci"},{"issue":"7","key":"pone.0341179.ref030","doi-asserted-by":"crossref","first-page":"1989","DOI":"10.1093\/jn\/132.7.1989","article-title":"Medium-chain fatty acids but not L-carnitine accelerate the kinetics of [14C]triacylglycerol utilization by colostrum-deprived newborn pigs","volume":"132","author":"KN Heo","year":"2002","journal-title":"J Nutr"},{"issue":"4","key":"pone.0341179.ref031","doi-asserted-by":"crossref","first-page":"442","DOI":"10.3390\/nu3040442","article-title":"Perspectives on immunoglobulins in colostrum and milk","volume":"3","author":"WL Hurley","year":"2011","journal-title":"Nutrients"},{"issue":"12","key":"pone.0341179.ref032","doi-asserted-by":"crossref","first-page":"3259","DOI":"10.1093\/jn\/131.12.3259","article-title":"Development of intestinal immunoglobulin absorption and enzyme activities in neonatal pigs is diet dependent","volume":"131","author":"AR Jensen","year":"2001","journal-title":"J Nutr"},{"issue":"8","key":"pone.0341179.ref033","doi-asserted-by":"crossref","first-page":"1350","DOI":"10.3390\/ani13081350","article-title":"Gut Health and Influencing Factors in Pigs","volume":"13","author":"C Szab\u00f3","year":"2023","journal-title":"Animals (Basel)"},{"issue":"6","key":"pone.0341179.ref034","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1080\/21655979.2016.1206168","article-title":"Xanthine dehydrogenase: An old enzyme with new knowledge and prospects","volume":"7","author":"C-H Wang","year":"2016","journal-title":"Bioengineered"},{"key":"pone.0341179.ref035","article-title":"Organellar Contacts of Milk Lipid Droplets","volume":"3","author":"J Monks","year":"2020","journal-title":"Contact (Thousand Oaks)"},{"issue":"6","key":"pone.0341179.ref036","doi-asserted-by":"crossref","first-page":"2215","DOI":"10.1046\/j.1523-1755.2000.00082.x","article-title":"XDH gene mutation is the underlying cause of classical xanthinuria: a second report","volume":"57","author":"D Levartovsky","year":"2000","journal-title":"Kidney Int"},{"issue":"6","key":"pone.0341179.ref037","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1007\/s10534-020-00252-2","article-title":"Heme-binding ability of bovine milk proteins","volume":"33","author":"K Orino","year":"2020","journal-title":"Biometals"},{"issue":"25","key":"pone.0341179.ref038","doi-asserted-by":"crossref","first-page":"9201","DOI":"10.1080\/14767058.2021.2021177","article-title":"Neonatal hyperbilirubinemia and the role of unbound bilirubin","volume":"35","author":"T Hegyi","year":"2022","journal-title":"J Matern Fetal Neonatal Med"},{"issue":"1","key":"pone.0341179.ref039","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1186\/s12967-025-06341-5","article-title":"Comprehensive review of the expanding roles of the carnitine pool in metabolic physiology: beyond fatty acid oxidation","volume":"23","author":"F Xiang","year":"2025","journal-title":"J Transl Med"},{"issue":"2","key":"pone.0341179.ref040","article-title":"Expression of apical membrane L-glutamate transporters in neonatal porcine epithelial cells along the small intestinal crypt-villus axis","volume":"287","author":"MZ Fan","year":"2004","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"issue":"25","key":"pone.0341179.ref041","doi-asserted-by":"crossref","first-page":"38681","DOI":"10.18632\/oncotarget.9583","article-title":"EAAT3 promotes amino acid transport and proliferation of porcine intestinal epithelial cells","volume":"7","author":"J-L Ye","year":"2016","journal-title":"Oncotarget"},{"issue":"1","key":"pone.0341179.ref042","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1152\/physrev.00018.2006","article-title":"Amino acid transport across mammalian intestinal and renal epithelia","volume":"88","author":"S Br\u00f6er","year":"2008","journal-title":"Physiol Rev"},{"key":"pone.0341179.ref043","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.tvjl.2019.02.006","article-title":"Blood glucose, amino acid profiles and nutrient transporter gene expressions in the small intestine of low and normal birthweight piglets during the early suckling period","volume":"247","author":"B Thongsong","year":"2019","journal-title":"Vet J"},{"issue":"11","key":"pone.0341179.ref044","doi-asserted-by":"crossref","first-page":"2859","DOI":"10.1093\/jn\/125.11.2859","article-title":"Developmental changes of free amino acid concentrations in fetal fluids of pigs","volume":"125","author":"G Wu","year":"1995","journal-title":"J Nutr"},{"issue":"8","key":"pone.0341179.ref045","doi-asserted-by":"crossref","first-page":"1843","DOI":"10.1093\/jn\/nxac095","article-title":"Lysine Dipeptide Enhances Gut Structure and Whole-Body Protein Synthesis in Neonatal Piglets with Intestinal Atrophy","volume":"152","author":"D Kirupananthan","year":"2022","journal-title":"J Nutr"},{"issue":"3","key":"pone.0341179.ref046","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1161\/CIRCRESAHA.120.318003","article-title":"Endothelial Cell Receptors in Tissue Lipid Uptake and Metabolism","volume":"128","author":"NA Abumrad","year":"2021","journal-title":"Circ 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