{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T09:36:03Z","timestamp":1769160963973,"version":"3.49.0"},"reference-count":18,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T00:00:00Z","timestamp":1769040000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Inner Mongolia Autonomous Region Science and Technology Innovation Guidance Project"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Gastric cancer (GC) is a major global health burden with limited treatment options. Identifying the molecular mechanisms underlying GC progression is critical for developing novel therapeutic strategies. We integrated whole-genome bisulfite sequencing and bulk RNA sequencing to identify hub genes involved in GC. Functional annotations were performed using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses, supplemented by data from The Cancer Genome Atlas. Key findings were validated using western blotting, immunohistochemistry, quantitative real-time PCR, and methylation-specific PCR. DExH-box helicase 58 (\n                    <jats:italic>DHX58<\/jats:italic>\n                    ) was identified as a hypomethylated, highly expressed hub gene in GC. Mechanistically,\n                    <jats:italic>DHX58<\/jats:italic>\n                    expression is regulated by the transcription factor CCAAT enhancer\u2013binding protein \u03b1. Immune profiling further implicated\n                    <jats:italic>DHX58<\/jats:italic>\n                    in the development of resistance to GC immunotherapy. Our study revealed that\n                    <jats:italic>DHX58<\/jats:italic>\n                    hypomethylation drives its overexpression in GC, making it a promising therapeutic target. These findings offer new insights into the pathogenesis of GC and suggest potential immunotherapeutic approaches.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0341230","type":"journal-article","created":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T18:28:10Z","timestamp":1769106490000},"page":"e0341230","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrative genomic analysis reveals DHX58 as a key player in gastric 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