{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T18:01:19Z","timestamp":1790704879147,"version":"4.1.0"},"reference-count":84,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["25-14-00223"],"award-info":[{"award-number":["25-14-00223"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Tumor Necrosis Factor Superfamily (TNFSF) comprises 20 members of membrane\/soluble signaling proteins regulating cell survival, cell proliferation\/differentiation, and innate\/adaptive immunity. Targeting signaling of TNFSF members is used clinically to treat several autoimmune and oncological diseases, and bone loss. They and their cognate receptors are in clinical trials as targets for treatment of autoimmune, inflammatory, oncological and other diseases. Recently, some representatives of S100 family of pleiotropic calcium-binding proteins were shown to interact with TNFSF members TNF and TRAIL, thereby suppressing their activity. In this work, we explored selectivity of interactions between soluble forms of 13 TNFSF members and 21 non-fused S100 proteins using surface plasmon resonance spectroscopy. A total of 27 interactions were found between CD70, CD30L, 4\u22121BBL, TWEAK, APRIL, LIGHT, VEGI and AITRL and Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -loaded forms of S100A1\/A2\/A4\/A5\/A6\/A12\/A16\/B\/P proteins, with equilibrium dissociation constants from 2 nM to 24 \u03bcM. Removal of calcium leads to disruption of the interactions. Molecular docking indicates presence of well-conserved binding sites of the both interaction partners. Mutagenesis of S100P evidences involvement of its \u2018hinge\u2019 region in binding of CD30L, VEGI and AITRL, as well as F89 residue in VEGI recognition. The revealed network of interactions is potentially important for regulation of the cellular communication mediated by TNFSF\/S100 proteins, which could be exploited for targeted therapy of socially significant diseases.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0341400","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T17:38:40Z","timestamp":1790703520000},"page":"e0341400","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Network of interactions between the tumor necrosis factor superfamily members and small S100 proteins"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8801-3786","authenticated-orcid":true,"given":"Victoria A.","family":"Rastrygina","sequence":"first","affiliation":[{"name":"Institute for Biological Instrumentation of the Russian Academy of Sciences","place":["Moscow Region, Russia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evgenia I.","family":"Deryusheva","sequence":"additional","affiliation":[{"name":"Institute for Biological Instrumentation of the Russian Academy of Sciences","place":["Moscow Region, Russia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexey S.","family":"Kazakov","sequence":"additional","affiliation":[{"name":"Institute for Biological Instrumentation of the Russian Academy of Sciences","place":["Moscow Region, Russia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrey S.","family":"Sokolov","sequence":"additional","affiliation":[{"name":"Institute for Biological Instrumentation of the Russian Academy of Sciences","place":["Moscow Region, Russia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria E.","family":"Permyakova","sequence":"additional","affiliation":[{"name":"Institute for Biological Instrumentation of the Russian Academy of Sciences","place":["Moscow Region, Russia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7459-6295","authenticated-orcid":true,"given":"Ekaterina A.","family":"Litus","sequence":"additional","affiliation":[{"name":"Institute for Biological Instrumentation of the Russian Academy of Sciences","place":["Moscow Region, Russia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4037-5857","authenticated-orcid":true,"given":"Vladimir N.","family":"Uversky","sequence":"additional","affiliation":[{"name":"Morsani College of Medicine, University of South Florida","place":["Tampa, Florida, United States of America"],"department":["Department of Molecular Medicine and USF Health Byrd Alzheimer\u2019s Research Institute"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9099-1148","authenticated-orcid":true,"given":"Eugene A.","family":"Permyakov","sequence":"additional","affiliation":[{"name":"Institute for Biological Instrumentation of the Russian Academy of Sciences","place":["Moscow Region, Russia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4086-3137","authenticated-orcid":true,"given":"Sergei E.","family":"Permyakov","sequence":"additional","affiliation":[{"name":"Institute for Biological Instrumentation of the Russian Academy of Sciences","place":["Moscow Region, Russia"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,9,29]]},"reference":[{"issue":"8","key":"pone.0341400.ref001","doi-asserted-by":"crossref","first-page":"1427","DOI":"10.1016\/S0006-2952(03)00493-3","article-title":"TWE-PRIL; a fusion protein of TWEAK and APRIL","volume":"66","author":"GM Kolfschoten","year":"2003","journal-title":"Biochem Pharmacol"},{"issue":"1","key":"pone.0341400.ref002","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1152\/physrev.00045.2017","article-title":"The TNF Family of Ligands and Receptors: Communication Modules in the Immune System and Beyond","volume":"99","author":"C Dostert","year":"2019","journal-title":"Physiol Rev"},{"issue":"1","key":"pone.0341400.ref003","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/S0968-0004(01)01995-8","article-title":"The molecular architecture of the TNF superfamily","volume":"27","author":"J-L Bodmer","year":"2002","journal-title":"Trends Biochem Sci"},{"issue":"4","key":"pone.0341400.ref004","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/S0092-8674(01)00237-9","article-title":"The TNF and TNF receptor superfamilies: integrating mammalian biology","volume":"104","author":"RM Locksley","year":"2001","journal-title":"Cell"},{"issue":"9","key":"pone.0341400.ref005","doi-asserted-by":"crossref","first-page":"3666","DOI":"10.1073\/pnas.72.9.3666","article-title":"An endotoxin-induced serum factor that causes necrosis of tumors","volume":"72","author":"EA Carswell","year":"1975","journal-title":"Proc Natl Acad Sci U S A"},{"key":"pone.0341400.ref006","doi-asserted-by":"crossref","first-page":"1133828","DOI":"10.3389\/fonc.2023.1133828","article-title":"Current comprehensive understanding of denosumab (the RANKL neutralizing antibody) in the treatment of bone metastasis of malignant tumors, including pharmacological mechanism and clinical trials","volume":"13","author":"J Lu","year":"2023","journal-title":"Front Oncol"},{"issue":"9","key":"pone.0341400.ref007","doi-asserted-by":"crossref","first-page":"2673","DOI":"10.1111\/all.14704","article-title":"Systematic review of safety and efficacy of belimumab in treating immune-mediated disorders","volume":"76","author":"C Kaegi","year":"2021","journal-title":"Allergy"},{"key":"pone.0341400.ref008","doi-asserted-by":"crossref","first-page":"103291","DOI":"10.1016\/j.jaut.2024.103291","article-title":"Telitacicept: A novel horizon in targeting autoimmunity and rheumatic diseases","volume":"148","author":"L Zeng","year":"2024","journal-title":"J Autoimmun"},{"issue":"3","key":"pone.0341400.ref009","doi-asserted-by":"crossref","DOI":"10.3390\/cancers17030496","article-title":"Antibody-Drug Conjugates Targeting CD30 in T-Cell Lymphomas: Clinical Progression and Mechanism","volume":"17","author":"Y Jiang","year":"2025","journal-title":"Cancers"},{"issue":"1","key":"pone.0341400.ref010","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1038\/s41408-025-01212-0","article-title":"The clinical journey of belantamab mafodotin in relapsed or refractory multiple myeloma: lessons in drug development","volume":"15","author":"P Mukhopadhyay","year":"2025","journal-title":"Blood Cancer J"},{"issue":"12","key":"pone.0341400.ref011","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1038\/s41573-024-01053-9","article-title":"Targeting the TNF and TNFR superfamilies in autoimmune disease and cancer","volume":"23","author":"M Croft","year":"2024","journal-title":"Nat Rev Drug Discov"},{"issue":"11","key":"pone.0341400.ref012","doi-asserted-by":"crossref","DOI":"10.1084\/jem.20240806","article-title":"Targeting TNF\/TNFR superfamilies in immune-mediated inflammatory diseases","volume":"221","author":"PK Veerasubramanian","year":"2024","journal-title":"J Exp Med"},{"issue":"4","key":"pone.0341400.ref013","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1007\/s10555-024-10206-6","article-title":"Tumor necrosis factor superfamily signaling: life and death in cancer","volume":"43","author":"O Ababneh","year":"2024","journal-title":"Cancer Metastasis Rev"},{"key":"pone.0341400.ref014","article-title":"SCOPe: improvements to the structural classification of proteins - extended database to facilitate variant interpretation and machine learning","volume":"50","author":"J-M Chandonia","year":"2022","journal-title":"Nucleic Acids Res"},{"key":"pone.0341400.ref015","first-page":"1","volume-title":"Reverse Signalling","author":"E Ross","year":"2016"},{"key":"pone.0341400.ref016","doi-asserted-by":"crossref","first-page":"107823","DOI":"10.1016\/j.compbiolchem.2023.107823","article-title":"Computational analyses of the interactome between TNF and TNFR superfamilies","volume":"103","author":"K Dhusia","year":"2023","journal-title":"Comput Biol Chem"},{"key":"pone.0341400.ref017","doi-asserted-by":"crossref","first-page":"143667","DOI":"10.1016\/j.ijbiomac.2025.143667","article-title":"Soluble form of tumor necrosis factor-related apoptosis-inducing ligand interacts with S100P protein","volume":"311","author":"VA Rastrygina","year":"2025","journal-title":"Int J Biol Macromol"},{"issue":"24","key":"pone.0341400.ref018","doi-asserted-by":"crossref","first-page":"15956","DOI":"10.3390\/ijms232415956","article-title":"Specific S100 Proteins Bind Tumor Necrosis Factor and Inhibit Its Activity","volume":"23","author":"AS Kazakov","year":"2022","journal-title":"Int J Mol Sci"},{"issue":"7","key":"pone.0341400.ref019","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1016\/S1357-2725(01)00046-2","article-title":"S100: a multigenic family of calcium-modulated proteins of the EF-hand type with intracellular and extracellular functional roles","volume":"33","author":"R Donato","year":"2001","journal-title":"Int J Biochem Cell Biol"},{"issue":"1","key":"pone.0341400.ref020","doi-asserted-by":"crossref","first-page":"24","DOI":"10.2174\/156652413804486214","article-title":"Functions of S100 proteins","volume":"13","author":"R Donato","year":"2013","journal-title":"Curr Mol Med"},{"key":"pone.0341400.ref021","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/bs.acc.2020.02.006","article-title":"S100 family proteins in inflammation and beyond","volume":"98","author":"G Sreejit","year":"2020","journal-title":"Adv Clin Chem"},{"issue":"15","key":"pone.0341400.ref022","doi-asserted-by":"crossref","first-page":"2274","DOI":"10.3390\/cells11152274","article-title":"Multifunctional Role of S100 Protein Family in the Immune System: An Update","volume":"11","author":"P Singh","year":"2022","journal-title":"Cells"},{"issue":"6","key":"pone.0341400.ref023","doi-asserted-by":"crossref","first-page":"118677","DOI":"10.1016\/j.bbamcr.2020.118677","article-title":"Role of S100 proteins in health and disease","volume":"1867","author":"LL Gonzalez","year":"2020","journal-title":"Biochim Biophys Acta Mol Cell Res"},{"key":"pone.0341400.ref024","doi-asserted-by":"crossref","DOI":"10.1002\/0470028637.met046","article-title":"3D Structures of the Calcium and Zinc Binding S100 Proteins","volume-title":"Handbook of Metalloproteins","author":"G Fritz","year":"2004"},{"issue":"3","key":"pone.0341400.ref025","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1073\/pnas.69.3.581","article-title":"Structure of a calcium-binding carp myogen","volume":"69","author":"CE Nockolds","year":"1972","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"8","key":"pone.0341400.ref026","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1007\/s11427-016-5088-4","article-title":"Binding of transition metals to S100 proteins","volume":"59","author":"BA Gilston","year":"2016","journal-title":"Sci China Life Sci"},{"issue":"4","key":"pone.0341400.ref027","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1007\/s00726-010-0540-4","article-title":"The role of zinc in the S100 proteins: insights from the X-ray structures","volume":"41","author":"OV Moroz","year":"2011","journal-title":"Amino Acids"},{"key":"pone.0341400.ref028","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkac1052","article-title":"UniProt: the Universal Protein Knowledgebase in 2023","volume":"51","author":"UniProt Consortium","year":"2023","journal-title":"Nucleic Acids Res"},{"issue":"1","key":"pone.0341400.ref029","doi-asserted-by":"crossref","first-page":"107689","DOI":"10.1016\/j.jsb.2020.107689","article-title":"Divalent cations influence the dimerization mode of murine S100A9 protein by modulating its disulfide bond pattern","volume":"213","author":"L Signor","year":"2021","journal-title":"J Struct Biol"},{"key":"pone.0341400.ref030","doi-asserted-by":"crossref","first-page":"3063","DOI":"10.1083\/jcb.109.6.3063","article-title":"Neurite extension and neuronal survival activities of recombinant S100 beta proteins that differ in the content and position of cysteine residues","volume":"109","author":"F Winningham-Major","year":"1989","journal-title":"J Cell Biol"},{"issue":"22","key":"pone.0341400.ref031","doi-asserted-by":"crossref","first-page":"4578","DOI":"10.1111\/j.1742-4658.2010.07887.x","article-title":"Natural and amyloid self-assembly of S100 proteins: structural basis of functional diversity","volume":"277","author":"G Fritz","year":"2010","journal-title":"FEBS J"},{"key":"pone.0341400.ref032","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1042\/BJ20060195","article-title":"Calcium-dependent and -independent interactions of the S100 protein family","volume":"396","author":"L Santamaria-Kisiel","year":"2006","journal-title":"Biochem J"},{"issue":"9","key":"pone.0341400.ref033","doi-asserted-by":"crossref","first-page":"1345","DOI":"10.3390\/biom13091345","article-title":"Interaction of S100A6 Protein with the Four-Helical Cytokines","volume":"13","author":"AS Kazakov","year":"2023","journal-title":"Biomolecules"},{"key":"pone.0341400.ref034","doi-asserted-by":"crossref","first-page":"67","DOI":"10.3389\/fphar.2012.00067","article-title":"S100 calcium binding proteins and ion channels","volume":"3","author":"A Hermann","year":"2012","journal-title":"Front Pharmacol"},{"issue":"3","key":"pone.0341400.ref035","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1006\/geno.1996.0563","article-title":"Genetic analysis of the epidermal differentiation complex (EDC) on human chromosome 1q21: chromosomal orientation, new markers, and a 6-Mb YAC contig","volume":"37","author":"I Marenholz","year":"1996","journal-title":"Genomics"},{"issue":"5035","key":"pone.0341400.ref036","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1126\/science.1957167","article-title":"Chromosome aberrations and cancer","volume":"254","author":"E Solomon","year":"1991","journal-title":"Science"},{"issue":"8","key":"pone.0341400.ref037","doi-asserted-by":"crossref","DOI":"10.1111\/exd.15158","article-title":"An overview of S100 proteins and their functions in skin homeostasis, interface dermatitis conditions and other skin pathologies","volume":"33","author":"W Abdi","year":"2024","journal-title":"Exp Dermatol"},{"issue":"2","key":"pone.0341400.ref038","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1038\/nrc3893","article-title":"S100 proteins in cancer","volume":"15","author":"AR Bresnick","year":"2015","journal-title":"Nat Rev Cancer"},{"issue":"8","key":"pone.0341400.ref039","doi-asserted-by":"crossref","first-page":"2037","DOI":"10.3390\/cancers12082037","article-title":"Friend or Foe: S100 Proteins in Cancer","volume":"12","author":"C Allg\u00f6wer","year":"2020","journal-title":"Cancers (Basel)"},{"issue":"1","key":"pone.0341400.ref040","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1007\/s12672-024-00945-x","article-title":"The S100 family is a prognostic biomarker and correlated with immune cell infiltration in pan-cancer","volume":"15","author":"X Liang","year":"2024","journal-title":"Discov Oncol"},{"key":"pone.0341400.ref041","doi-asserted-by":"crossref","first-page":"110156","DOI":"10.1016\/j.biopha.2020.110156","article-title":"Roles of S100 family members in drug resistance in tumors: Status and prospects","volume":"127","author":"X Hua","year":"2020","journal-title":"Biomed Pharmacother"},{"issue":"5","key":"pone.0341400.ref042","doi-asserted-by":"crossref","first-page":"103295","DOI":"10.1016\/j.autrev.2023.103295","article-title":"Circulating Calprotectin (cCLP) in autoimmune diseases","volume":"22","author":"M Manfredi","year":"2023","journal-title":"Autoimmun Rev"},{"issue":"1","key":"pone.0341400.ref043","doi-asserted-by":"crossref","first-page":"403","DOI":"10.3390\/ijms26010403","article-title":"Roles of the Receptor for Advanced Glycation End Products and Its Ligands in the Pathogenesis of Alzheimer\u2019s Disease","volume":"26","author":"W Li","year":"2025","journal-title":"Int J Mol Sci"},{"key":"pone.0341400.ref044","doi-asserted-by":"crossref","first-page":"5488591","DOI":"10.1155\/2021\/5488591","article-title":"The S100 Protein Family as Players and Therapeutic Targets in Pulmonary Diseases","volume":"2021","author":"Z Sattar","year":"2021","journal-title":"Pulm Med"},{"key":"pone.0341400.ref045","doi-asserted-by":"crossref","first-page":"114348","DOI":"10.1016\/j.intimp.2025.114348","article-title":"Role of S100A1, S100A4, S100A8\/A9 and S100B in myocardial infarction and heart failure","volume":"151","author":"B Cheng","year":"2025","journal-title":"Int Immunopharmacol"},{"issue":"1","key":"pone.0341400.ref046","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1186\/s10020-023-00662-1","article-title":"S100 proteins in cardiovascular diseases","volume":"29","author":"Y Zhou","year":"2023","journal-title":"Mol Med"},{"issue":"3","key":"pone.0341400.ref047","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1111\/imm.13722","article-title":"Roles of S100A8, S100A9 and S100A12 in infection, inflammation and immunity","volume":"171","author":"P Xia","year":"2024","journal-title":"Immunology"},{"issue":"6","key":"pone.0341400.ref048","doi-asserted-by":"crossref","first-page":"1617","DOI":"10.1007\/s12551-018-0471-y","article-title":"S100 proteins as therapeutic targets","volume":"10","author":"AR Bresnick","year":"2018","journal-title":"Biophys Rev"},{"issue":"19","key":"pone.0341400.ref049","doi-asserted-by":"crossref","first-page":"6640","DOI":"10.3390\/molecules27196640","article-title":"S100 Proteins as Novel Therapeutic Targets in Psoriasis and Other Autoimmune Diseases","volume":"27","author":"K Kurpet","year":"2022","journal-title":"Molecules"},{"key":"pone.0341400.ref050","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1007\/978-1-4939-9030-6_43","article-title":"Serum S100B Levels in Melanoma","volume":"1929","author":"AL Frauchiger","year":"2019","journal-title":"Methods Mol Biol"},{"issue":"13","key":"pone.0341400.ref051","doi-asserted-by":"crossref","first-page":"6476","DOI":"10.3390\/ijms26136476","article-title":"Calprotectin as a Biomarker for Infectious Diseases: A Comparative Review with Conventional Inflammatory Markers","volume":"26","author":"K Sejersen","year":"2025","journal-title":"Int J Mol Sci"},{"issue":"1","key":"pone.0341400.ref052","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1080\/07391102.2015.1132392","article-title":"Interleukin-11 binds specific EF-hand proteins via their conserved structural motifs","volume":"35","author":"AS Kazakov","year":"2017","journal-title":"J Biomol Struct Dyn"},{"issue":"24","key":"pone.0341400.ref053","doi-asserted-by":"crossref","first-page":"9473","DOI":"10.3390\/ijms21249473","article-title":"Interferon Beta Activity Is Modulated via Binding of Specific S100 Proteins","volume":"21","author":"AS Kazakov","year":"2020","journal-title":"Int J Mol Sci"},{"issue":"1","key":"pone.0341400.ref054","doi-asserted-by":"crossref","first-page":"120","DOI":"10.3390\/biom12010120","article-title":"Erythropoietin Interacts with Specific S100 Proteins","volume":"12","author":"AS Kazakov","year":"2022","journal-title":"Biomolecules"},{"key":"pone.0341400.ref055","doi-asserted-by":"crossref","first-page":"102869","DOI":"10.1016\/j.ceca.2024.102869","article-title":"Recognition of granulocyte-macrophage colony-stimulating factor by specific S100 proteins","volume":"119","author":"AS Kazakov","year":"2024","journal-title":"Cell Calcium"},{"issue":"4","key":"pone.0341400.ref056","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1016\/j.bbrc.2015.11.024","article-title":"High-affinity interaction between interleukin-11 and S100P protein","volume":"468","author":"AS Kazakov","year":"2015","journal-title":"Biochem Biophys Res Commun"},{"issue":"19","key":"pone.0341400.ref057","doi-asserted-by":"crossref","first-page":"12000","DOI":"10.3390\/ijms231912000","article-title":"Calcium-Bound S100P Protein Is a Promiscuous Binding Partner of the Four-Helical Cytokines","volume":"23","author":"AS Kazakov","year":"2022","journal-title":"Int J Mol Sci"},{"issue":"2","key":"pone.0341400.ref058","doi-asserted-by":"crossref","first-page":"830","DOI":"10.1016\/0006-291X(82)90712-4","article-title":"Ca2+-induced hydrophobic site on calmodulin: application for purification of calmodulin by phenyl-Sepharose affinity chromatography","volume":"104","author":"R Gopalakrishna","year":"1982","journal-title":"Biochem Biophys Res Commun"},{"issue":"8","key":"pone.0341400.ref059","doi-asserted-by":"crossref","first-page":"1158","DOI":"10.3390\/biom11081158","article-title":"Strontium Binding to \u03b1-Parvalbumin, a Canonical Calcium-Binding Protein of the \u201cEF-Hand\u201d Family","volume":"11","author":"AA Vologzhannikova","year":"2021","journal-title":"Biomolecules"},{"issue":"11","key":"pone.0341400.ref060","doi-asserted-by":"crossref","first-page":"2411","DOI":"10.1002\/pro.5560041120","article-title":"How to measure and predict the molar absorption coefficient of a protein","volume":"4","author":"CN Pace","year":"1995","journal-title":"Protein Sci"},{"issue":"9","key":"pone.0341400.ref061","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1016\/j.str.2020.06.006","article-title":"Performance and Its Limits in Rigid Body Protein-Protein Docking","volume":"28","author":"IT Desta","year":"2020","journal-title":"Structure"},{"issue":"1","key":"pone.0341400.ref062","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1093\/nar\/28.1.235","article-title":"The Protein Data Bank","volume":"28","author":"HM Berman","year":"2000","journal-title":"Nucleic Acids Res"},{"issue":"8016","key":"pone.0341400.ref063","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1038\/s41586-024-07487-w","article-title":"Accurate structure prediction of biomolecular interactions with AlphaFold 3","volume":"630","author":"J Abramson","year":"2024","journal-title":"Nature"},{"key":"pone.0341400.ref064","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkae241","article-title":"The EMBL-EBI Job Dispatcher sequence analysis tools framework in 2024","volume":"52","author":"F Madeira","year":"2024","journal-title":"Nucleic Acids Res"},{"key":"pone.0341400.ref065","volume-title":"The PyMOL Molecular Graphics System, Version 1.8","author":"LLC Schrodinger","year":"2015"},{"issue":"10","key":"pone.0341400.ref066","doi-asserted-by":"crossref","first-page":"5022","DOI":"10.1074\/jbc.M115.683946","article-title":"Binding Studies of TNF Receptor Superfamily (TNFRSF) Receptors on Intact Cells","volume":"291","author":"I Lang","year":"2016","journal-title":"J Biol Chem"},{"issue":"6","key":"pone.0341400.ref067","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1016\/j.bbamcr.2008.11.016","article-title":"Binding of S100 proteins to RAGE: an update","volume":"1793","author":"E Leclerc","year":"2009","journal-title":"Biochim Biophys Acta"},{"key":"pone.0341400.ref068","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1042\/bj2840905","article-title":"Oligomeric tumour necrosis factor alpha slowly converts into inactive forms at bioactive levels","author":"A Corti","year":"1992","journal-title":"Biochem J"},{"issue":"1","key":"pone.0341400.ref069","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1023\/A:1011320027914","article-title":"Calmodulin target database","volume":"1","author":"KL Yap","year":"2000","journal-title":"J Struct Funct Genomics"},{"key":"pone.0341400.ref070","author":"M Ferdinand","year":"1938"},{"key":"pone.0341400.ref071","doi-asserted-by":"crossref","first-page":"102520","DOI":"10.1016\/j.ceca.2021.102520","article-title":"Specific cytokines of interleukin-6 family interact with S100 proteins","volume":"101","author":"AS Kazakov","year":"2022","journal-title":"Cell Calcium"},{"issue":"1","key":"pone.0341400.ref072","doi-asserted-by":"crossref","first-page":"5904","DOI":"10.1038\/s41598-022-09574-2","article-title":"Promiscuity mapping of the S100 protein family using a high-throughput holdup assay","volume":"12","author":"MA Simon","year":"2022","journal-title":"Sci Rep"},{"issue":"13","key":"pone.0341400.ref073","doi-asserted-by":"crossref","first-page":"2834","DOI":"10.1111\/febs.15175","article-title":"High-throughput competitive fluorescence polarization assay reveals functional redundancy in the S100 protein family","volume":"287","author":"MA Simon","year":"2020","journal-title":"FEBS J"},{"key":"pone.0341400.ref074","article-title":"The IntAct database: efficient access to fine-grained molecular interaction data","volume":"50","author":"N Del Toro","year":"2022","journal-title":"Nucleic Acids Res"},{"issue":"1","key":"pone.0341400.ref075","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1002\/pro.3978","article-title":"The BioGRID database: A comprehensive biomedical resource of curated protein, genetic, and chemical interactions","volume":"30","author":"R Oughtred","year":"2021","journal-title":"Protein Sci"},{"key":"pone.0341400.ref076","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1007\/978-1-60761-444-9_19","article-title":"The PeptideAtlas Project","volume":"604","author":"EW Deutsch","year":"2010","journal-title":"Methods Mol Biol"},{"issue":"7","key":"pone.0341400.ref077","doi-asserted-by":"crossref","first-page":"2164","DOI":"10.1039\/C0MB00305K","article-title":"Intrinsic disorder in S100 proteins","volume":"7","author":"SE Permyakov","year":"2011","journal-title":"Mol Biosyst"},{"issue":"8","key":"pone.0341400.ref078","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0103947","article-title":"Structural insights into calcium-bound S100P and the V domain of the RAGE complex","volume":"9","author":"SR Penumutchu","year":"2014","journal-title":"PLoS One"},{"issue":"3","key":"pone.0341400.ref079","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1023\/B:JNMR.0000032617.88899.4b","article-title":"Letter to the Editor: NMR Structure of the Apo-S100P Protein","volume":"29","author":"Y-C Lee","year":"2004","journal-title":"J Biomol NMR"},{"issue":"4","key":"pone.0341400.ref080","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1016\/S0022-2836(02)01278-0","article-title":"The crystal structure at 2A resolution of the Ca2+ -binding protein S100P","volume":"325","author":"H Zhang","year":"2003","journal-title":"J Mol Biol"},{"issue":"4","key":"pone.0341400.ref081","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1016\/j.bbapap.2010.01.011","article-title":"PONDR-FIT: a meta-predictor of intrinsically disordered amino acids","volume":"1804","author":"B Xue","year":"2010","journal-title":"Biochim Biophys Acta"},{"key":"pone.0341400.ref082","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1038\/msb.2011.75","article-title":"Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega","volume":"7","author":"F Sievers","year":"2011","journal-title":"Mol Syst Biol"},{"issue":"7873","key":"pone.0341400.ref083","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly accurate protein structure prediction with AlphaFold","volume":"596","author":"J Jumper","year":"2021","journal-title":"Nature"},{"issue":"15","key":"pone.0341400.ref084","doi-asserted-by":"crossref","first-page":"2072","DOI":"10.1093\/bioinformatics\/bts302","article-title":"SALIGN: a web server for alignment of multiple protein sequences and structures","volume":"28","author":"H Braberg","year":"2012","journal-title":"Bioinformatics"}],"container-title":["PLOS One"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0341400","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T17:38:54Z","timestamp":1790703534000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0341400"}},"subtitle":[],"editor":[{"given":"Andreas","family":"Hofmann","sequence":"first","affiliation":[{"name":"Max Rubner-Institut the Federal Research Institute of Nutrition and Food: Max Rubner-Institut Bundesforschungsinstitut fur Ernahrung und Lebensmittel","place":["GERMANY"]}],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,9,29]]},"references-count":84,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9,29]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0341400","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,29]]}}}