{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:03:06Z","timestamp":1787022186379,"version":"build-2736575974"},"reference-count":65,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T00:00:00Z","timestamp":1773360000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R01HL143885"],"award-info":[{"award-number":["R01HL143885"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R01HL098445"],"award-info":[{"award-number":["R01HL098445"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["in collaboration with the University of Alabama at Birmingham (75N92023D00002 & 75N92023D00005), Northwestern University (75N92023D00004), University of Minnesota (75N92023D00006), and Kaiser Foundation Research Institute (75N92023D00003)"],"award-info":[{"award-number":["in collaboration with the University of Alabama at Birmingham (75N92023D00002 & 75N92023D00005), Northwestern University (75N92023D00004), University of Minnesota (75N92023D00006), and Kaiser Foundation Research Institute (75N92023D00003)"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100020298","name":"Balchem","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100020298","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100023078","name":"State of North Carolina","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100023078","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>Metabolic-associated steatotic liver disease (MASLD) is marked by accumulation of hepatic triacylglycerols (TAG), but many other lipids have been implicated. Choline metabolism has been shown to be related to MASLD, specifically through phosphatidylcholines (PC) role in hepatic TAG removal through very low density lipoproteins (VLDL). There are a lack of population-based studies with integrated data on lipidomics, choline metabolites, and MASLD. We tested associations between the plasma lipidome, choline metabolites, and MASLD using data from the Coronary Artery Risk Development in Young Adults (CARDIA) Study. The analytic sample included 1,039 participants with data on choline metabolite, lipidomic, and liver attenuation data [mean (SD) age: 45 (4); 57% female; 57% White race]. MASLD (n\u2009=\u2009234) was defined as mean CT-derived liver attenuation\u2009&lt;\u200951HU. Plasma lipidomics and choline metabolites were quantified from stored fasting plasma using liquid-chromatography and infusion-mass spectrometry. In logistic regression adjusted for sociodemographics, lifestyle, and clinical variables, total TAGs, diacylglycerols (DAG), and dihydroceramides (DCER) were positively, and lactosylceramides (LCER) were inversely, associated with MASLD. Species-level results revealed diverging MASLD associations for PCs, based on FA composition. In choline metabolite models, betaine was inversely associated with MASLD. A lipidomic risk score (LRS) derived from penalized regression of MASLD on lipid species was associated positively with choline, and inversely with betaine. We contribute population-based results to a growing literature relating lipidomics and MASLD. In our data, FA composition is biologically relevant to MASLD, particularly for PCs and TAGs. Our results link choline metabolites to both the plasma lipidome and to incident MASLD, furthering efforts in biomarker development and supporting mechanistic evidence using population-level data.<\/jats:p>","DOI":"10.1371\/journal.pone.0341462","type":"journal-article","created":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T17:22:41Z","timestamp":1773422561000},"page":"e0341462","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":2,"title":["Plasma lipidomics, choline metabolites, and metabolic-associated steatotic liver disease (MASLD): A Coronary Artery Risk Development in Young Adults (CARDIA) study"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-5731-7324","authenticated-orcid":true,"given":"Jessica 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Vvedenskaya","year":"2021","journal-title":"J Lipid Res"},{"issue":"2","key":"pone.0341462.ref018","first-page":"122","article-title":"A serum lipidomic study of patients with non-alcoholic fatty liver disease","volume":"25","author":"RX Yang","year":"2017","journal-title":"Zhonghua Gan Zang Bing Za Zhi"},{"issue":"12","key":"pone.0341462.ref019","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1038\/s41575-021-00502-9","article-title":"Metabolomics and lipidomics in NAFLD: biomarkers and non-invasive diagnostic tests","volume":"18","author":"M Masoodi","year":"2021","journal-title":"Nat Rev Gastroenterol Hepatol"},{"issue":"7","key":"pone.0341462.ref020","article-title":"Plasma phospholipids and fatty acid composition differ between liver biopsy-proven nonalcoholic fatty liver disease and healthy subjects","volume":"6","author":"DWL Ma","year":"2016","journal-title":"Nutr 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Retention strategies over 25 years in the Coronary Artery Risk Development in Young Adults (CARDIA) Study","volume":"9","author":"E Funkhouser","year":"2017","journal-title":"Contemp Clin Trials Commun"},{"issue":"2","key":"pone.0341462.ref030","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/0003-2697(90)90449-J","article-title":"The measurement of dimethylamine, trimethylamine, and trimethylamine N-oxide using capillary gas chromatography-mass spectrometry","volume":"187","author":"KA daCosta","year":"1990","journal-title":"Anal Biochem"},{"issue":"3","key":"pone.0341462.ref031","doi-asserted-by":"crossref","first-page":"778","DOI":"10.3945\/ajcn.114.087692","article-title":"Effect of egg ingestion on trimethylamine-N-oxide production in humans: a randomized, controlled, dose-response study","volume":"100","author":"CA Miller","year":"2014","journal-title":"Am J Clin Nutr"},{"key":"pone.0341462.ref032","first-page":"3","volume-title":"Complex Lipids Targeted Panel","year":"2021"},{"issue":"12","key":"pone.0341462.ref033","doi-asserted-by":"crossref","first-page":"1539","DOI":"10.1194\/jlr.S120001025","article-title":"Update on LIPID MAPS classification, nomenclature, and shorthand notation for MS-derived lipid structures","volume":"61","author":"G Liebisch","year":"2020","journal-title":"J Lipid Res"},{"issue":"4","key":"pone.0341462.ref034","doi-asserted-by":"crossref","DOI":"10.1161\/JAHA.119.014279","article-title":"Longitudinal Association of Non-Alcoholic Fatty Liver Disease With Changes in Myocardial Structure and Function: The CARDIA Study","volume":"9","author":"LB VanWagner","year":"2020","journal-title":"J Am Heart Assoc"},{"issue":"11","key":"pone.0341462.ref035","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1097\/00008483-198911000-00003","article-title":"Validity and Reliability of Short Physical Activity History: Cardia and the Minnesota Heart Health Program","volume":"9","author":"DR Jacobs","year":"1989","journal-title":"J Cardiopulm Rehabil"},{"issue":"9","key":"pone.0341462.ref036","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.1016\/S0002-8223(21)01299-2","article-title":"The CARDIA dietary history: development, implementation, and evaluation","volume":"91","author":"A McDonald","year":"1991","journal-title":"J Am Diet Assoc"},{"issue":"3","key":"pone.0341462.ref037","doi-asserted-by":"crossref","first-page":"580","DOI":"10.3945\/ajcn.111.020719","article-title":"Longitudinal trends in diet and effects of sex, race, and education on dietary quality score change: the Coronary Artery Risk Development in Young Adults study","volume":"95","author":"FPC Sijtsma","year":"2012","journal-title":"Am J Clin Nutr"},{"issue":"4","key":"pone.0341462.ref038","doi-asserted-by":"crossref","first-page":"622","DOI":"10.1053\/j.ajkd.2010.02.337","article-title":"Estimating GFR using the CKD Epidemiology Collaboration (CKD-EPI) creatinine equation: more accurate GFR estimates, lower CKD prevalence estimates, and better risk predictions","volume":"55","author":"AS Levey","year":"2010","journal-title":"Am J Kidney Dis"},{"issue":"11","key":"pone.0341462.ref039","doi-asserted-by":"crossref","first-page":"2117","DOI":"10.2337\/dc19-0100","article-title":"High-Coverage Targeted Lipidomics Reveals Novel Serum Lipid Predictors and Lipid Pathway Dysregulation Antecedent to Type 2 Diabetes Onset in Normoglycemic Chinese Adults","volume":"42","author":"J Lu","year":"2019","journal-title":"Diabetes Care"},{"issue":"1","key":"pone.0341462.ref040","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1007\/s10618-012-0295-5","article-title":"Training and assessing classification rules with imbalanced data","volume":"28","author":"G Menardi","year":"2012","journal-title":"Data Min Knowl Disc"},{"key":"pone.0341462.ref041","doi-asserted-by":"crossref","unstructured":"Lunardon N, Menardi G, Torelli N. 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resistance: a potential mechanism for hepatoprotection by betaine","volume":"299","author":"E Kathirvel","year":"2010","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"key":"pone.0341462.ref048","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.ejphar.2019.01.043","article-title":"Betaine modulates oxidative stress, inflammation, apoptosis, autophagy, and Akt\/mTOR signaling in methionine-choline deficiency-induced fatty liver disease","volume":"848","author":"M Veskovic","year":"2019","journal-title":"Eur J Pharmacol"},{"issue":"8","key":"pone.0341462.ref049","doi-asserted-by":"crossref","first-page":"1366","DOI":"10.3390\/medicina60081366","article-title":"Use of a Micronutrient Cocktail to Improve Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) in Adults with Obesity: A Randomized, Double-Blinded Pilot Clinical Trial","volume":"60","author":"IT Perva","year":"2024","journal-title":"Medicina 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